Mapping of a gene involved in distorted segregation in interspecific tomato populations
Introduction. Distorted segregation (SD) occurs when the expected genotypes do not correspond to those observed, which favors single parent alleles. This phenomenon was observed in intermediate populations from the cross between Solanum pimpinellifolium and the Moneymaker cultivar of Solanum lycopersicum, developed during the construction process of a library of introgression lines. Objective. Obtain informative recombinants that allow physically mapping a region with SD associated with the wild Solanum pimpinellifolium species. Materials and methods. The research was carried out at the Institute of Molecular and Cellular Biology of Plants (IBMCP) attached to the Higher Council for Scientific Research (CSIC) based at the Universidad Politecnica de Valencia, Spain. A population of 2000 plants was screened to identify recombinants in that region, with a modification of the high-resolution melting technique (HRM-Multiplex). These recombinants were self-fertilized, and through the Chi-square statistic it was determined whether SNP markers identified within the target region had a normal (1:2:1) or distorted segregation for each informative recombinant selected. Results. Fifty-four informative recombinants were generated and identified, grouped into 10 bins according to the physical recombination site. It was possible to delimit the region with distorted segregation until obtaining a final size of 84 Kb, which was located at the distal end of the long arm of chromosome 4. This region contains a large number of genes, some of which are related to fertilization processes, sterility and cell division among others, which could be related to the studied phenomenon. Conclusion. A gene was found, that causes a segregation distortion in an interval of 84 Kb and possibly is the gene Ge described by Rick in 1966.
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Universidad de Costa Rica
2020
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Barrantes Santamaría, Walter Granell, Antonio Monforte, Antonio Jose |
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Barrantes Santamaría, Walter Granell, Antonio Monforte, Antonio Jose Mapping of a gene involved in distorted segregation in interspecific tomato populations |
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Barrantes Santamaría, Walter Granell, Antonio Monforte, Antonio Jose |
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Barrantes Santamaría, Walter |
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Mapping of a gene involved in distorted segregation in interspecific tomato populations |
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Mapping of a gene involved in distorted segregation in interspecific tomato populations |
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Mapping of a gene involved in distorted segregation in interspecific tomato populations |
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Mapping of a gene involved in distorted segregation in interspecific tomato populations |
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Mapping of a gene involved in distorted segregation in interspecific tomato populations |
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mapping of a gene involved in distorted segregation in interspecific tomato populations |
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Introduction. Distorted segregation (SD) occurs when the expected genotypes do not correspond to those observed, which favors single parent alleles. This phenomenon was observed in intermediate populations from the cross between Solanum pimpinellifolium and the Moneymaker cultivar of Solanum lycopersicum, developed during the construction process of a library of introgression lines. Objective. Obtain informative recombinants that allow physically mapping a region with SD associated with the wild Solanum pimpinellifolium species. Materials and methods. The research was carried out at the Institute of Molecular and Cellular Biology of Plants (IBMCP) attached to the Higher Council for Scientific Research (CSIC) based at the Universidad Politecnica de Valencia, Spain. A population of 2000 plants was screened to identify recombinants in that region, with a modification of the high-resolution melting technique (HRM-Multiplex). These recombinants were self-fertilized, and through the Chi-square statistic it was determined whether SNP markers identified within the target region had a normal (1:2:1) or distorted segregation for each informative recombinant selected. Results. Fifty-four informative recombinants were generated and identified, grouped into 10 bins according to the physical recombination site. It was possible to delimit the region with distorted segregation until obtaining a final size of 84 Kb, which was located at the distal end of the long arm of chromosome 4. This region contains a large number of genes, some of which are related to fertilization processes, sterility and cell division among others, which could be related to the studied phenomenon. Conclusion. A gene was found, that causes a segregation distortion in an interval of 84 Kb and possibly is the gene Ge described by Rick in 1966. |
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oai:portal.ucr.ac.cr:article394852023-06-16T13:45:47Z Mapping of a gene involved in distorted segregation in interspecific tomato populations Mapeo de un gen implicado en segregación distorsionada en poblaciones interespecíficas de tomate Barrantes Santamaría, Walter Granell, Antonio Monforte, Antonio Jose genetic markers massive genotyping high resolution fusion curves recombination marcadores genéticos genotipo masivo curvas de fusione alta resolución recombinación Introduction. Distorted segregation (SD) occurs when the expected genotypes do not correspond to those observed, which favors single parent alleles. This phenomenon was observed in intermediate populations from the cross between Solanum pimpinellifolium and the Moneymaker cultivar of Solanum lycopersicum, developed during the construction process of a library of introgression lines. Objective. Obtain informative recombinants that allow physically mapping a region with SD associated with the wild Solanum pimpinellifolium species. Materials and methods. The research was carried out at the Institute of Molecular and Cellular Biology of Plants (IBMCP) attached to the Higher Council for Scientific Research (CSIC) based at the Universidad Politecnica de Valencia, Spain. A population of 2000 plants was screened to identify recombinants in that region, with a modification of the high-resolution melting technique (HRM-Multiplex). These recombinants were self-fertilized, and through the Chi-square statistic it was determined whether SNP markers identified within the target region had a normal (1:2:1) or distorted segregation for each informative recombinant selected. Results. Fifty-four informative recombinants were generated and identified, grouped into 10 bins according to the physical recombination site. It was possible to delimit the region with distorted segregation until obtaining a final size of 84 Kb, which was located at the distal end of the long arm of chromosome 4. This region contains a large number of genes, some of which are related to fertilization processes, sterility and cell division among others, which could be related to the studied phenomenon. Conclusion. A gene was found, that causes a segregation distortion in an interval of 84 Kb and possibly is the gene Ge described by Rick in 1966. Introducción. La segregación distorsionada (SD) ocurre cuando los genotipos esperados no corresponden a los observados, lo que favorece alelos de un solo parental. Este fenómeno se observó en poblaciones intermedias provenientes del cruce entre Solanum pimpinellifolium y el cultivar Moneymaker deSolanum lycopersicum, desarrolladas durante el proceso de construcción de una genoteca de líneas de introgresión. Objetivo. Obtener recombinantes informativos que permitan mapear físicamente una región con SD asociado a la especie silvestre Solanum pimpinellifolium. Materiales y métodos. La investigación se desarrolló en el Instituto de Biología Molecular y Celular de Plantas (IBMCP) adscrito al Consejo Superior de Investigaciones Científicas (CSIC) con sede en la Universidad Politécnica de Valencia, España. Se cribó una población de 2000 plantas para identificar recombinantes en dicha región, con una modificación de la técnica fusión de alta resolución (high resolution melting, HRM-Multiplex). Estos recombinantes se autofecundaron, y mediante el estadístico Chi-cuadrado se determinó si marcadores SNP identificados dentro de la región diana tenían una segregación normal (1:2:1) o distorsionada para cada recombinante informativo seleccionado. Resultados. Se generaron e identificaron 54 recombinantes informativos, que se agruparon en 10 bins de acuerdo con el sitio físico de recombinación. Se logró acotar la región con segregación distorsionada hasta obtener un tamaño final de 84 Kb, la cual se localizó en el extremo distal del brazo largo del cromosoma 4. Esta región contiene gran cantidad de genes, algunos de los cuales están relacionados con procesos de fecundación, esterilidad y división celular entre otros, que podrían estar relacionados con el fenómeno estudiado. Conclusión. Se localizó un gen que provoca una distorsión en la segregación en un intervalo de 84 Kb y que posiblemente sea el gen Ge descrito por Rick en 1966. Universidad de Costa Rica 2020-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article text ¨texto text/html application/pdf application/epub+zip audio/mpeg audio/mpeg text/xml https://revistas.ucr.ac.cr/index.php/agromeso/article/view/39485 10.15517/am.v31i2.39485 Agronomía Mesoamericana; 2020: Agronomía Mesoamericana: Vol. 31, Issue 2 (May-August); 419-431 Agronomía Mesoamericana; 2020: Agronomía Mesoamericana: Vol. 31, Nº 2 (mayo-agosto); 419-431 Agronomía Mesoamericana; 2020: Agronomía Mesoamericana: Vol. 31, Issue 2 (May-August); 419-431 2215-3608 1021-7444 spa eng https://revistas.ucr.ac.cr/index.php/agromeso/article/view/39485/42095 https://revistas.ucr.ac.cr/index.php/agromeso/article/view/39485/42096 https://revistas.ucr.ac.cr/index.php/agromeso/article/view/39485/42097 https://revistas.ucr.ac.cr/index.php/agromeso/article/view/39485/42098 https://revistas.ucr.ac.cr/index.php/agromeso/article/view/39485/42099 https://revistas.ucr.ac.cr/index.php/agromeso/article/view/39485/42236 |