SEQUENCES OF GENE BOLA-DRB3.2 FROM THE GUAYMI AND GUABALA CREOLE CATTLE OF PANAMA
The major histocompatibility complex (MHC) has been associated with economically important traits such as milk production, protein and fat in milk and with resistance or susceptibility to diseases such as brucellosis, tick infestation, hemoparasites and mastitis. The second exon of the BoLA-DRB3 gene of CMH is highly polymorphic and 136 alleles that encode functional restriction elements have been reported, through which a lymphocyte can recognize an antigen as its own or foreign. The objective of this work was to validate a protocol for the amplification and sequencing of the second exon of the BoLA-DRB3 gene in Guaymi and Guabala bovine populations to carry out characterization studies in Panama. The obtained sequences showed identity with the second exon of the BoLA-DRB3.2 gene that oscillated between 87% and 98%, in terms of the number of base pairs (bp), these ranged between 148 and 239 and maximum score (MAX SCORE) between 165 to 339. The most frequent alleles in the Guabala breed were, *R-73 (21%) and *1801 (14%) and in the Guaymi breed it was *0101 (38%). Regarding shared alleles, it was observed that both breeds share the alleles, *0101 and *R-73. From the point of view of the conservation and use of biodiversity, the Guaymi and Guabala breeds represent a potential reserve of genes that must be studied and promote their use within programs that require minimum use of inputs or, within crossbrreeding schemes where highly rustic animals of the Bos taurus x Bos taurus type are required.
| Main Authors: | , |
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| Format: | Digital revista |
| Language: | spa |
| Published: |
Instituto de Innovación Agropecuaria de Panamá
2018
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| Online Access: | http://www.revistacienciaagropecuaria.ac.pa/index.php/ciencia-agropecuaria/article/view/2 |
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