Effect of reduced glutathione (GSH) on the sperm quality of verraco under subhumid tropic conditions

Effect of reduced glutathione (GSH) on the sperm quality of verraco under subhumid tropic conditions

In this work was evaluate the effect of reduced glutathione (GSH) on sperm quality of boars under tropic conditions. 40 ejaculates from 4 boars (10 replicates per boar) of commercial lines Yorkshire / Landrace and Pietrain / Duroc between 24 and 28 months of age, were collected at 7 day intervals by the gloved hand technique.The ejaculate was diluted 1:10 with 2000 x106 (viable sperm), L-Reduced Glutathione (GSH, Sigma-Aldrich ®) added to the diluent half conservation (MIII ®). Three groups were formed, control group without GSH (0GSH) and two treatments with GSH concentrations 1 mM (1GSH) and 2 mM (2GSH), total mobility, integrity of the plasma membrane, osmotic resistance and acrosomal integrity initial and in the semen conserved at 17º C was evaluated at 0, 24, 48, 72 and 96 h. The plasma membrane integrity of boar sperm supplemented with 2GSH was 88.0% and 1GSH of 87.3%, different with respect 0GSH (P <0.05). In total mobility, in 2GSH and 1GSH was 58.9% and 56.9%, respectively, showing differences (P <0.05) with respect to the 0GSH (52.0%). During the conservation period from 72 and to 96 h of storage, total mobility showed a 14% decrease for 0GSH, while that in IGSH nad 2GSH only a decrease of 11 and 6% respectively, was observed. Plasma membrane integrity at 96 h was 87.52%, 85.40% and 80.65% for 2GSH, 1GSH and 0GSH, respectively (P<0.05). The variables osmotic tolerance and acrosomal integrity not show significant differences (P> 0.05) in between treatments during the time of conservation. The supplementation with 2 mM of GSH to the dilution medium improvement the quality of spermatozoa of boars maintained in tropical conditions conserved at 17° C

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Main Authors: Abrego-Salgado, Nancy Patricia, Herrera-Camacho, Jose, Estrada-Paqui, Efren, Tinoco-Magaña, Juan Carlos, Bautista Hernandez, Miguel Ángel
Format: Digital revista
Language:spa
Published: Asociacion Latinoamericana de Produccion Animal 2023
Online Access:https://ojs.alpa.uy/index.php/ojs_files/article/view/3185
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countrycode UY
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databasecode rev-alpa
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language spa
format Digital
author Abrego-Salgado, Nancy Patricia
Herrera-Camacho, Jose
Estrada-Paqui, Efren
Tinoco-Magaña, Juan Carlos
Bautista Hernandez, Miguel Ángel
spellingShingle Abrego-Salgado, Nancy Patricia
Herrera-Camacho, Jose
Estrada-Paqui, Efren
Tinoco-Magaña, Juan Carlos
Bautista Hernandez, Miguel Ángel
Effect of reduced glutathione (GSH) on the sperm quality of verraco under subhumid tropic conditions
author_facet Abrego-Salgado, Nancy Patricia
Herrera-Camacho, Jose
Estrada-Paqui, Efren
Tinoco-Magaña, Juan Carlos
Bautista Hernandez, Miguel Ángel
author_sort Abrego-Salgado, Nancy Patricia
title Effect of reduced glutathione (GSH) on the sperm quality of verraco under subhumid tropic conditions
title_short Effect of reduced glutathione (GSH) on the sperm quality of verraco under subhumid tropic conditions
title_full Effect of reduced glutathione (GSH) on the sperm quality of verraco under subhumid tropic conditions
title_fullStr Effect of reduced glutathione (GSH) on the sperm quality of verraco under subhumid tropic conditions
title_full_unstemmed Effect of reduced glutathione (GSH) on the sperm quality of verraco under subhumid tropic conditions
title_sort effect of reduced glutathione (gsh) on the sperm quality of verraco under subhumid tropic conditions
description In this work was evaluate the effect of reduced glutathione (GSH) on sperm quality of boars under tropic conditions. 40 ejaculates from 4 boars (10 replicates per boar) of commercial lines Yorkshire / Landrace and Pietrain / Duroc between 24 and 28 months of age, were collected at 7 day intervals by the gloved hand technique.The ejaculate was diluted 1:10 with 2000 x106 (viable sperm), L-Reduced Glutathione (GSH, Sigma-Aldrich ®) added to the diluent half conservation (MIII ®). Three groups were formed, control group without GSH (0GSH) and two treatments with GSH concentrations 1 mM (1GSH) and 2 mM (2GSH), total mobility, integrity of the plasma membrane, osmotic resistance and acrosomal integrity initial and in the semen conserved at 17º C was evaluated at 0, 24, 48, 72 and 96 h. The plasma membrane integrity of boar sperm supplemented with 2GSH was 88.0% and 1GSH of 87.3%, different with respect 0GSH (P <0.05). In total mobility, in 2GSH and 1GSH was 58.9% and 56.9%, respectively, showing differences (P <0.05) with respect to the 0GSH (52.0%). During the conservation period from 72 and to 96 h of storage, total mobility showed a 14% decrease for 0GSH, while that in IGSH nad 2GSH only a decrease of 11 and 6% respectively, was observed. Plasma membrane integrity at 96 h was 87.52%, 85.40% and 80.65% for 2GSH, 1GSH and 0GSH, respectively (P<0.05). The variables osmotic tolerance and acrosomal integrity not show significant differences (P> 0.05) in between treatments during the time of conservation. The supplementation with 2 mM of GSH to the dilution medium improvement the quality of spermatozoa of boars maintained in tropical conditions conserved at 17° C
publisher Asociacion Latinoamericana de Produccion Animal
publishDate 2023
url https://ojs.alpa.uy/index.php/ojs_files/article/view/3185
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spelling oai:ojs.ojs.alpa.uy:article-31852023-06-19T17:38:10Z Effect of reduced glutathione (GSH) on the sperm quality of verraco under subhumid tropic conditions Efecto del glutatión reducido (GSH) sobre la calidad espermática de verraco bajo condiciones de trópico subhúmedo Efeito da glutação reduzida (GSH) sobre a qualidade espermática de verraco bajo condiciones de trópico subhúmedo Abrego-Salgado, Nancy Patricia Herrera-Camacho, Jose Estrada-Paqui, Efren Tinoco-Magaña, Juan Carlos Bautista Hernandez, Miguel Ángel GSH preserved semen boar GSH SEMEN CONSERVADO VERRACO GSH pork In this work was evaluate the effect of reduced glutathione (GSH) on sperm quality of boars under tropic conditions. 40 ejaculates from 4 boars (10 replicates per boar) of commercial lines Yorkshire / Landrace and Pietrain / Duroc between 24 and 28 months of age, were collected at 7 day intervals by the gloved hand technique.The ejaculate was diluted 1:10 with 2000 x106 (viable sperm), L-Reduced Glutathione (GSH, Sigma-Aldrich ®) added to the diluent half conservation (MIII ®). Three groups were formed, control group without GSH (0GSH) and two treatments with GSH concentrations 1 mM (1GSH) and 2 mM (2GSH), total mobility, integrity of the plasma membrane, osmotic resistance and acrosomal integrity initial and in the semen conserved at 17º C was evaluated at 0, 24, 48, 72 and 96 h. The plasma membrane integrity of boar sperm supplemented with 2GSH was 88.0% and 1GSH of 87.3%, different with respect 0GSH (P <0.05). In total mobility, in 2GSH and 1GSH was 58.9% and 56.9%, respectively, showing differences (P <0.05) with respect to the 0GSH (52.0%). During the conservation period from 72 and to 96 h of storage, total mobility showed a 14% decrease for 0GSH, while that in IGSH nad 2GSH only a decrease of 11 and 6% respectively, was observed. Plasma membrane integrity at 96 h was 87.52%, 85.40% and 80.65% for 2GSH, 1GSH and 0GSH, respectively (P<0.05). The variables osmotic tolerance and acrosomal integrity not show significant differences (P> 0.05) in between treatments during the time of conservation. The supplementation with 2 mM of GSH to the dilution medium improvement the quality of spermatozoa of boars maintained in tropical conditions conserved at 17° C Se evaluó el efecto del Glutatión reducido (GSH) sobre la calidad espermática de verracos bajo condiciones de trópico subhúmedo. Se utilizaron 40 eyaculados de 4 verracos (10 réplicas por verraco) de líneas comerciales Yorkshire/Landrace y Pietrain/Duroc entre 24 y 28 meses de edad, colectados a intervalos de 7 días por la técnica de mano enguantada. El eyaculado fue diluido 1:10 con 2000 x106 (espermatozoides viables), se utilizó L-Glutatión Reducido (GSH, Sigma-Aldrich®) adicionado al diluente media duración (MIII®). Se formaron 3 grupos, grupo control sin GSH (0GHS) y dos tratamientos con GSH a concentración 1 Mm (1GSH) y 2 mM (2GSH), se evaluó la movilidad total, integridad de la membrana plasmática, resistencia osmótica e integridad acrosomal inicial y en el semen conservado a 17º C a las 0, 24, 48, 72 y 96 h. La integridad de membrana plasmática de los espermatozoides en 2GSH fue 88.0% y en 1GSH de 87.3%, diferentes con respecto a 0GSH (P<0.05). En la movilidad total, en 2GHS y IGHS fue 58.9% y 56.9%, respectivamente, mostrando diferencias (P<0.05) con respecto al 0GSH (52.0%), la integridad acrosomal y la tolerancia osmótica no mostraron diferencias entre tratamientos. Durante la conservación, a partir de las 72 y hasta 96 h, la movilidad total mostró una disminución del 14% para el grupo 0GSH, mientras que en 1GHS y 2GSH, fue de 11 y 6%, respectivamente. La integridad de membrana plasmática a las 96 h fue 87.52, 85.40% y 80.65% para 2GSH, 1GSH y 0GSH, respectivamente (P<0.05). Las variables tolerancia osmótica e integridad acrosomal no mostraron diferencias (P>0.05) entre tratamientos durante la conservación. La suplementación con 2 mM de GSH al medio de dilución mejora la calidad de espermatozoides de verracos mantenidos condiciones de trópico subhúmedo conservados a 17° C. O efeito da glutationa reduzida (GSH) na qualidade do esperma de cachaços foi avaliado em condições tropicais subúmidas. Foram utilizados 40 ejaculados de 4 javalis (10 réplicas por javali) das linhagens comerciais Yorkshire/Landrace e Pietrain/Duroc entre 24 e 28 meses de idade, coletados em intervalos de 7 dias pela técnica da mão enluvada. O ejaculado foi diluído 1:10 com 2000 x106 (esperma viável), L-Glutationa reduzida (GSH, Sigma-Aldrich®) foi adicionada ao diluente de média duração (MIII®). Foram formados três grupos, um grupo controle sem GSH (0GHS) e dois tratamentos com GSH na concentração de 1 Mm (1GSH) e 2 mM (2GSH), avaliaram-se motilidade total, integridade da membrana plasmática, resistência osmótica e integridade acrossomal inicial .em sêmen armazenado a 17ºC nas horas 0, 24, 48, 72 e 96 h. A integridade da membrana plasmática dos espermatozoides em 2GSH foi de 88,0% e em 1GSH de 87,3%, diferente em relação a 0GSH (P<0,05). Na mobilidade total, no 2GHS e IGHS foi de 58,9% e 56,9%, respectivamente, apresentando diferenças (P<0,05) com relação ao 0GSH (52,0%), integridade acrossomal e tolerância osmótica não apresentaram diferenças entre os tratamentos. Durante o armazenamento, de 72 e até 96 h, a mobilidade total apresentou queda de 14% para o grupo 0GSH, enquanto no 1GHS e 2GSH foi de 11 e 6%, respectivamente. A integridade da membrana plasmática em 96 h foi de 87,52, 85,40% e 80,65% para 2GSH, 1GSH e 0GSH, respectivamente (P<0,05). As variáveis ​​tolerância osmótica e integridade acrossômica não apresentaram diferenças (P>0,05) entre os tratamentos durante a conservação. A suplementação com 2 mM de GSH ao meio de diluição melhora a qualidade dos espermatozoides de cachaços mantidos em condições tropicais subúmidas mantidas a 17°C. Asociacion Latinoamericana de Produccion Animal 2023-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Articulo cientifico original Texto Texto application/pdf https://ojs.alpa.uy/index.php/ojs_files/article/view/3185 10.53588/alpa.310514 Latin American Archives of Animal Production; Vol. 31 No. Suplemento (2023): XXVII ALPA Meeting, Zacatecas, Mexico; 73-79 Archivos Latinoamericanos de Producción Animal; Vol. 31 Núm. Suplemento (2023): XXVII Reunion ALPA, Zacatecas, Mexico; 73-79 2075-8359 1022-1301 spa https://ojs.alpa.uy/index.php/ojs_files/article/view/3185/1812 Copyright (c) 2023 Nancy Patricia Abrego-Salgado, Jose Herrera-Camacho, Efren Estrada-Paqui, Juan Carlos Tinoco-Magaña, Miguel Ángel Bautista Hernandez https://creativecommons.org/licenses/by-nc-sa/4.0

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