EVALUATION OF AN IMMUNOHISTOCHEMICAL TECHNIQUE FOR THE DIAGNOSIS OF MIXED MAMMARY TUMORS IN BITCHES

EVALUATION OF AN IMMUNOHISTOCHEMICAL TECHNIQUE FOR THE DIAGNOSIS OF MIXED MAMMARY TUMORS IN BITCHES

The aim of the study was to evaluate an immunohistochemical technique for the diagnosis of canine mixed mammary tumors (CMMT) using anti-cytokeratin AE1/AE3 and anti-vimentin monoclonal antibodies that are routinely used in the diagnosis of human mammary tumors. Twenty paraffin tissue blocks of canine mixed mammary tumors, previously diagnosed by hematoxilin-eosin (HE) histopathology were used. Three slides sets, 4 μm thick, were obtained per viability of the tissue, and the other two were used for the immunohistochemistry through the Streptavidin-Biotin-labeled method. Epithelial cells positively reacted against the anti-cytokeratin AE1/AE3 showing a brown colour at the cytoplasma level. Fibroblasts, connective tissue, myoepithelial cells, cartilage cells and bone areas showed a brown colour against the anti-vimentin antibody. All benign tumors reacted positively to both antibodies, whereas 73% malign tumors were positive with the anti-cytokeratin antibody. It is concluded that mixed mammary neoplasm in dogs may be diagnosed by the technique of immunohistochemistry using monoclonal antibodies used in the detection of human mammary tumors. block. One was stained with HE to confirm the

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Main Authors: Rodríguez C., Cyntia, Santillán A., Gilberto, Perales C., Rosa, Chavera C., Alfonso
Format: Digital revista
Language:spa
Published: Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2013
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/2484
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spelling oai:ojs.csi.unmsm:article-24842020-03-31T00:25:13Z EVALUATION OF AN IMMUNOHISTOCHEMICAL TECHNIQUE FOR THE DIAGNOSIS OF MIXED MAMMARY TUMORS IN BITCHES EVALUACIÓN DE UNA TÉCNICA INMUNOHISTOQUÍMICA PARA EL DIAGNÓSTICO DE NEOPLASIAS MAMARIAS MIXTAS EN CANINOS Rodríguez C., Cyntia Santillán A., Gilberto Perales C., Rosa Chavera C., Alfonso Neoplasias mamarias inmunohistoquímica citoqueratina vimentina. mammary neoplasms immunohistochemistry cytokeratin vimentin The aim of the study was to evaluate an immunohistochemical technique for the diagnosis of canine mixed mammary tumors (CMMT) using anti-cytokeratin AE1/AE3 and anti-vimentin monoclonal antibodies that are routinely used in the diagnosis of human mammary tumors. Twenty paraffin tissue blocks of canine mixed mammary tumors, previously diagnosed by hematoxilin-eosin (HE) histopathology were used. Three slides sets, 4 μm thick, were obtained per viability of the tissue, and the other two were used for the immunohistochemistry through the Streptavidin-Biotin-labeled method. Epithelial cells positively reacted against the anti-cytokeratin AE1/AE3 showing a brown colour at the cytoplasma level. Fibroblasts, connective tissue, myoepithelial cells, cartilage cells and bone areas showed a brown colour against the anti-vimentin antibody. All benign tumors reacted positively to both antibodies, whereas 73% malign tumors were positive with the anti-cytokeratin antibody. It is concluded that mixed mammary neoplasm in dogs may be diagnosed by the technique of immunohistochemistry using monoclonal antibodies used in the detection of human mammary tumors. block. One was stained with HE to confirm the El objetivo del presente estudio fue evaluar una técnica inmunohistoquímica (IHQ) para el diagnóstico de neoplasias mamarias mixtas (NMM) en caninos, usando anticuerpos monoclonales anti-citoqueratina AE1/AE3 y anti-vimentina, que son empleados en el diagnóstico de neoplasias mamarias en humanos. Se tomaron 20 muestras de tejidos parafinados de neoplasias caninas diagnosticadas histológicamente por hematoxilina y eosina (HE) como NMM. Cada muestra fue cortada a 4 μm de espesor, obteniéndose tres láminas por muestra. Una fue coloreada con HE para confirmar la viabilidad del tejido y en las otras dos láminas se realizó la IHQ mediante el método de Streptavidina-Biotina. Las células epiteliales reaccionaron de manera positiva con el anticuerpo anti-citoqueratina AE1/AE3 dando una coloración marrón a nivel citoplasmático. El anticuerpo anti-vimentina reaccionó de manera positiva con los fibroblastos, el tejido conectivo, las células mioepiteliales y las células cartilaginosas, dando una coloración semejante al anterior. Todas las neoplasias benignas reaccionaron contra ambos anticuerpos, mientras que el 73% de las neoplasias malignas reaccionaron de manera positiva contra el anticuerpo anti-citoqueratina. Se concluye que las NMM en caninos pueden ser diagnosticadas mediante la técnica de IHQ usando los anticuerpos monoclonales empleados en humanos. Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2013-06-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/2484 10.15381/rivep.v24i2.2484 Revista de Investigaciones Veterinarias del Perú; Vol. 24 No. 2 (2013); 176-181 Revista de Investigaciones Veterinarias del Perú; Vol. 24 Núm. 2 (2013); 176-181 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/2484/2178 Derechos de autor 2013 Cyntia Rodríguez C., Gilberto Santillán A., Rosa Perales C., Alfonso Chavera C. https://creativecommons.org/licenses/by-nc-sa/4.0
institution UNMSM
collection OJS
country Perú
countrycode PE
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region America del Sur
libraryname Biblioteca de la Facultad de Medicina Veterinaria
language spa
format Digital
author Rodríguez C., Cyntia
Santillán A., Gilberto
Perales C., Rosa
Chavera C., Alfonso
spellingShingle Rodríguez C., Cyntia
Santillán A., Gilberto
Perales C., Rosa
Chavera C., Alfonso
EVALUATION OF AN IMMUNOHISTOCHEMICAL TECHNIQUE FOR THE DIAGNOSIS OF MIXED MAMMARY TUMORS IN BITCHES
author_facet Rodríguez C., Cyntia
Santillán A., Gilberto
Perales C., Rosa
Chavera C., Alfonso
author_sort Rodríguez C., Cyntia
title EVALUATION OF AN IMMUNOHISTOCHEMICAL TECHNIQUE FOR THE DIAGNOSIS OF MIXED MAMMARY TUMORS IN BITCHES
title_short EVALUATION OF AN IMMUNOHISTOCHEMICAL TECHNIQUE FOR THE DIAGNOSIS OF MIXED MAMMARY TUMORS IN BITCHES
title_full EVALUATION OF AN IMMUNOHISTOCHEMICAL TECHNIQUE FOR THE DIAGNOSIS OF MIXED MAMMARY TUMORS IN BITCHES
title_fullStr EVALUATION OF AN IMMUNOHISTOCHEMICAL TECHNIQUE FOR THE DIAGNOSIS OF MIXED MAMMARY TUMORS IN BITCHES
title_full_unstemmed EVALUATION OF AN IMMUNOHISTOCHEMICAL TECHNIQUE FOR THE DIAGNOSIS OF MIXED MAMMARY TUMORS IN BITCHES
title_sort evaluation of an immunohistochemical technique for the diagnosis of mixed mammary tumors in bitches
description The aim of the study was to evaluate an immunohistochemical technique for the diagnosis of canine mixed mammary tumors (CMMT) using anti-cytokeratin AE1/AE3 and anti-vimentin monoclonal antibodies that are routinely used in the diagnosis of human mammary tumors. Twenty paraffin tissue blocks of canine mixed mammary tumors, previously diagnosed by hematoxilin-eosin (HE) histopathology were used. Three slides sets, 4 μm thick, were obtained per viability of the tissue, and the other two were used for the immunohistochemistry through the Streptavidin-Biotin-labeled method. Epithelial cells positively reacted against the anti-cytokeratin AE1/AE3 showing a brown colour at the cytoplasma level. Fibroblasts, connective tissue, myoepithelial cells, cartilage cells and bone areas showed a brown colour against the anti-vimentin antibody. All benign tumors reacted positively to both antibodies, whereas 73% malign tumors were positive with the anti-cytokeratin antibody. It is concluded that mixed mammary neoplasm in dogs may be diagnosed by the technique of immunohistochemistry using monoclonal antibodies used in the detection of human mammary tumors. block. One was stained with HE to confirm the
publisher Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publishDate 2013
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/2484
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