Histological and molecular characterization of Edwardsiella anguillarum infection in tilapia (Oreochromis niloticus) cultivated in the biofloc system in Lima, Peru
The aim of this study was to isolate and characterize phenotypically and molecularly the pathogen Edwardsiella anguillarum, as well as to determine the anatomo-histopathological lesions in tilapia (Oreochromis niloticus) cultivated with biofloc technology in the northern area of Lima, Peru. Five isolated bacterial strains were isolated and characterized by conventional biochemical techniques and by the API 20E system. E. anguillarum was identified from internal organs using biochemical and molecular techniques. The most frequent external clinical signs were bilateral exophthalmia, erythema in the pectoral fins and around the anus. Internally, whitish nodules in the heart and liver were observed. The histopathological study revealed necrosis in the branchial lamellae, spleen, intestine, posterior kidney and gonad, as well as the presence of an inflammatory reaction of granulomatous type in the heart, liver, spleen, kidney and gonads. The strains showed fermentative glucose metabolism and positivity to methyl red, production of hydrogen sulphide, indole and acid production from mannitol. The isolates were confirmed by the PCR technique and sequencing of the 16S rRNA gene. All the strains showed sensitivity to the antibiotics nalidixic acid, florfenicol, gentamicin, kanamycin, flumequine, oxytetracycline and trimethoprim sulfamethoxazole.
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Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
2020
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Accès en ligne: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17097 |
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oai:ojs.csi.unmsm:article-170972020-12-21T21:52:38Z Histological and molecular characterization of Edwardsiella anguillarum infection in tilapia (Oreochromis niloticus) cultivated in the biofloc system in Lima, Peru Caracterización histológica y molecular de infección por Edwardsiella anguillarum en tilapia (Oreochromis niloticus) cultivada en sistema biofloc en Lima, Perú Sierralta, Veronica Anamaría Mayta H., Egma León Q., Jorge Serna C., José Robles R., Christian Alfaro A., Rubén Edwardsiella anguillarum PCR Biofloc Tilapia Edwardsiella anguillarum PCR Biofloc Tilapia The aim of this study was to isolate and characterize phenotypically and molecularly the pathogen Edwardsiella anguillarum, as well as to determine the anatomo-histopathological lesions in tilapia (Oreochromis niloticus) cultivated with biofloc technology in the northern area of Lima, Peru. Five isolated bacterial strains were isolated and characterized by conventional biochemical techniques and by the API 20E system. E. anguillarum was identified from internal organs using biochemical and molecular techniques. The most frequent external clinical signs were bilateral exophthalmia, erythema in the pectoral fins and around the anus. Internally, whitish nodules in the heart and liver were observed. The histopathological study revealed necrosis in the branchial lamellae, spleen, intestine, posterior kidney and gonad, as well as the presence of an inflammatory reaction of granulomatous type in the heart, liver, spleen, kidney and gonads. The strains showed fermentative glucose metabolism and positivity to methyl red, production of hydrogen sulphide, indole and acid production from mannitol. The isolates were confirmed by the PCR technique and sequencing of the 16S rRNA gene. All the strains showed sensitivity to the antibiotics nalidixic acid, florfenicol, gentamicin, kanamycin, flumequine, oxytetracycline and trimethoprim sulfamethoxazole. El objetivo del estudio fue aislar y caracterizar fenotípica y molecularmente al patógeno Edwardsiella anguillarum, así como determinar las lesiones anatomo-histopatológicas en tilapia (Oreochromis niloticus) cultivada con tecnología biofloc en la zona norte de Lima, Perú. Se trabajó con cinco cepas bacterianas aisladas y caracterizadas mediante técnicas bioquímicas convencionales y por el sistema API 20E. Se identificó E. anguillarum a partir de órganos internos mediante las técnicas bioquímica y molecular. Los signos clínicos externos más frecuentes fueron exoftalmia bilateral, eritema en aletas pectorales y alrededor del ano. Internamente se apreció nodulaciones blanquecinas en el corazón e hígado. El estudio histopatológico reveló necrosis en lamelas branquiales, bazo, intestino, riñón posterior y gónada, así como presencia de reacción inflamatoria de tipo granulomatosa en corazón, hígado, bazo, riñón y gónadas. Las cepas evaluadas presentaron metabolismo fermentativo de glucosa y positividad ante las pruebas de rojo de metilo, producción de sulfuro de hidrógeno, indol y ácido a partir de manitol. Los aislados fueron confirmados por la técnica de PCR y secuenciación del gen 16S ARNr. Todas las cepas presentaron sensibilidad a los antibióticos ácido nalidíxico, florfenicol, gentamicina, kanamicina, flumequina, oxitetraciclina y sulfatrimetoprim. Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2020-12-21 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17097 10.15381/rivep.v31i4.17097 Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 4 (2020); e17097 Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 4 (2020); e17097 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17097/16139 Derechos de autor 2020 Veronica Anamaría Sierralta, Egma Mayta H., Jorge León Q., José Serna C., Christian Robles R., Rubén Alfaro A. https://creativecommons.org/licenses/by-nc-sa/4.0 |
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Sierralta, Veronica Anamaría Mayta H., Egma León Q., Jorge Serna C., José Robles R., Christian Alfaro A., Rubén |
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Sierralta, Veronica Anamaría Mayta H., Egma León Q., Jorge Serna C., José Robles R., Christian Alfaro A., Rubén Histological and molecular characterization of Edwardsiella anguillarum infection in tilapia (Oreochromis niloticus) cultivated in the biofloc system in Lima, Peru |
author_facet |
Sierralta, Veronica Anamaría Mayta H., Egma León Q., Jorge Serna C., José Robles R., Christian Alfaro A., Rubén |
author_sort |
Sierralta, Veronica Anamaría |
title |
Histological and molecular characterization of Edwardsiella anguillarum infection in tilapia (Oreochromis niloticus) cultivated in the biofloc system in Lima, Peru |
title_short |
Histological and molecular characterization of Edwardsiella anguillarum infection in tilapia (Oreochromis niloticus) cultivated in the biofloc system in Lima, Peru |
title_full |
Histological and molecular characterization of Edwardsiella anguillarum infection in tilapia (Oreochromis niloticus) cultivated in the biofloc system in Lima, Peru |
title_fullStr |
Histological and molecular characterization of Edwardsiella anguillarum infection in tilapia (Oreochromis niloticus) cultivated in the biofloc system in Lima, Peru |
title_full_unstemmed |
Histological and molecular characterization of Edwardsiella anguillarum infection in tilapia (Oreochromis niloticus) cultivated in the biofloc system in Lima, Peru |
title_sort |
histological and molecular characterization of edwardsiella anguillarum infection in tilapia (oreochromis niloticus) cultivated in the biofloc system in lima, peru |
description |
The aim of this study was to isolate and characterize phenotypically and molecularly the pathogen Edwardsiella anguillarum, as well as to determine the anatomo-histopathological lesions in tilapia (Oreochromis niloticus) cultivated with biofloc technology in the northern area of Lima, Peru. Five isolated bacterial strains were isolated and characterized by conventional biochemical techniques and by the API 20E system. E. anguillarum was identified from internal organs using biochemical and molecular techniques. The most frequent external clinical signs were bilateral exophthalmia, erythema in the pectoral fins and around the anus. Internally, whitish nodules in the heart and liver were observed. The histopathological study revealed necrosis in the branchial lamellae, spleen, intestine, posterior kidney and gonad, as well as the presence of an inflammatory reaction of granulomatous type in the heart, liver, spleen, kidney and gonads. The strains showed fermentative glucose metabolism and positivity to methyl red, production of hydrogen sulphide, indole and acid production from mannitol. The isolates were confirmed by the PCR technique and sequencing of the 16S rRNA gene. All the strains showed sensitivity to the antibiotics nalidixic acid, florfenicol, gentamicin, kanamycin, flumequine, oxytetracycline and trimethoprim sulfamethoxazole. |
publisher |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
publishDate |
2020 |
url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17097 |
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