A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela

A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela

The aims of the study were to determine the prevalence of antibodies anti-E canis and the molecular detection of E. canis as well as to determine the species and level of tick infestations in dogs from a rural community in Aragua state, Venezuela. Blood samples from 110 domestic dogs were obtained and analyzed by Indirect Immunofluorescence (IFI) and Nested Polymerase Chain Reaction (PCR). The seroprevalence was 77.3% and the molecular detection was 45.2%. The percentage of dogs infested with ticks was 69%, mostly with Rhipicephalus sanguineus. The results showed that 80% (68/85) of seropositive dogs and 32% (8/25) of seronegative dogs were tick infested (OR=8.5, 95%, CI 3.19 to 22.6; p<0.00001).

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Main Authors: Martínez A., María del Carmen, Arraga-Alvarado, Cruz María, Triana-Alonso, Francisco Javier, Ruiz C., Johanny Alejandra, Gutiérrez G., Clara Nancy
Format: Digital revista
Language:spa
Published: Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2015
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11220
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spelling oai:ojs.csi.unmsm:article-112202020-03-30T01:10:42Z A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela Estudio Serológico y Molecular de Ehrlichia canis en Perros de una Comunidad del Estado Aragua, Venezuela Martínez A., María del Carmen Arraga-Alvarado, Cruz María Triana-Alonso, Francisco Javier Ruiz C., Johanny Alejandra Gutiérrez G., Clara Nancy Ehrlichia canis ehrlichiosis IFI PCR Rhipicephalus sanguineus Venezuela Ehrlichia canis ehrlichiosis IFI PCR Riphicephalus sanguineus Venezuela The aims of the study were to determine the prevalence of antibodies anti-E canis and the molecular detection of E. canis as well as to determine the species and level of tick infestations in dogs from a rural community in Aragua state, Venezuela. Blood samples from 110 domestic dogs were obtained and analyzed by Indirect Immunofluorescence (IFI) and Nested Polymerase Chain Reaction (PCR). The seroprevalence was 77.3% and the molecular detection was 45.2%. The percentage of dogs infested with ticks was 69%, mostly with Rhipicephalus sanguineus. The results showed that 80% (68/85) of seropositive dogs and 32% (8/25) of seronegative dogs were tick infested (OR=8.5, 95%, CI 3.19 to 22.6; p<0.00001). Los objetivos de esta investigación fueron determinar la prevalencia de anticuerpos anti-E. canis y la detección molecular de E. canis, así como determinar el nivel de infestación y especies de garrapatas en perros de una comunidad rural del estado Aragua, Venezuela. Se recolectaron muestras sanguíneas de 110 perros domésticos y fueron evaluadas por inmunofluorescencia indirecta (IFI) y por la técnica de reacción en cadena de la polimerasa anidada (PCR). La seroprevalencia resultó en 77.3% y la detección molecular fue de 45.2%. El porcentaje de perros infestados por garrapatas fue de 69%, identificándose mayormente a Riphicephalus sanguineus. Se determinó que 80% (68/85) de los perros seropositivos y el 32% (8/25) de los seronegativos estaban infestados por garrapatas (OR=8.5, IC 95%: 3.19-22.6; p<0.00001). Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2015-12-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11220 10.15381/rivep.v26i4.11220 Revista de Investigaciones Veterinarias del Perú; Vol. 26 No. 4 (2015); 648-656 Revista de Investigaciones Veterinarias del Perú; Vol. 26 Núm. 4 (2015); 648-656 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11220/10290 Derechos de autor 2015 María del Carmen Martínez A., Cruz María Arraga-Alvarado, Francisco Javier Triana-Alonso, Johanny Alejandra Ruiz C., Clara Nancy Gutiérrez G. https://creativecommons.org/licenses/by-nc-sa/4.0
institution UNMSM
collection OJS
country Perú
countrycode PE
component Revista
access En linea
databasecode rev-rivep
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Medicina Veterinaria
language spa
format Digital
author Martínez A., María del Carmen
Arraga-Alvarado, Cruz María
Triana-Alonso, Francisco Javier
Ruiz C., Johanny Alejandra
Gutiérrez G., Clara Nancy
spellingShingle Martínez A., María del Carmen
Arraga-Alvarado, Cruz María
Triana-Alonso, Francisco Javier
Ruiz C., Johanny Alejandra
Gutiérrez G., Clara Nancy
A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela
author_facet Martínez A., María del Carmen
Arraga-Alvarado, Cruz María
Triana-Alonso, Francisco Javier
Ruiz C., Johanny Alejandra
Gutiérrez G., Clara Nancy
author_sort Martínez A., María del Carmen
title A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela
title_short A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela
title_full A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela
title_fullStr A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela
title_full_unstemmed A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela
title_sort serological and molecular survey of ehrlichia canis in dogs from a community in aragua state, venezuela
description The aims of the study were to determine the prevalence of antibodies anti-E canis and the molecular detection of E. canis as well as to determine the species and level of tick infestations in dogs from a rural community in Aragua state, Venezuela. Blood samples from 110 domestic dogs were obtained and analyzed by Indirect Immunofluorescence (IFI) and Nested Polymerase Chain Reaction (PCR). The seroprevalence was 77.3% and the molecular detection was 45.2%. The percentage of dogs infested with ticks was 69%, mostly with Rhipicephalus sanguineus. The results showed that 80% (68/85) of seropositive dogs and 32% (8/25) of seronegative dogs were tick infested (OR=8.5, 95%, CI 3.19 to 22.6; p<0.00001).
publisher Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publishDate 2015
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11220
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