Sylvatic triatomines (Hemiptera: Reduviidae) in Bolivia : trends toward domesticity and possibleinfection with Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae)
ABSTRACT The risk of domestic transmission of Trypanosoma cruzi (Chagas) by sylvatic triatomines was assessed in an isolated area of the subandean region of Bolivia. None of the 390 residents examined had serological evidence of infection. Two sylvatic triatomine species, Eratyrus mucronatus (Stal) and Triatoma sordida (Stal), were found in houses and in peridomestic structures. The collection of nymphal instars of both species from some houses indicated possible domesticity. Microscopic examination of feces from 92 insects showed no parasites, and cultures from the guts of 30 insects were negative. Nevertheless, a polymerase chain reaction (PCR) test performed on the same fecal samples showed the presence of T. cruzi DNA in 19.1 and 12.5% of E. mucronatus and T. sordida, respectively. These 16 PCR positive samples were hybridized with 2 T. cruzi-specific probes known from the domestic cycle in Bolivia (clones 20 and 39). At least 1 of these clones was identified in 7 bugs (5 E. mucronatus and 2 T sordida). Moreover, no hybridization was observed with these probes in 8 E. mucronatus and 1 T sordida samples that showed an amplified band by PCR. These data indicated that T. cruzi clones, genetically unrelated to clones 20 and 39, also were circulating in this area. Based on these results, the 2 sylvatic triatomine species encountered in Apolo should not be overlooked as possible local vectors of T cruzi.
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Format: | Article biblioteca |
Language: | Spanish / Castilian |
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Journal of Medical Entomology
1995
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Subjects: | ERATYRUS MUCRONATUS, TRIATOMA SÓRDIDA, TRIATOMINOS SELVÁTICOS, TRYPANOSOMA CRUZI, BOLIVIA, |
Online Access: | http://repositorio.umsa.bo/xmlui/handle/123456789/27685 |
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oai:localhost:8080:123456789-276852022-04-01T13:35:43Z Sylvatic triatomines (Hemiptera: Reduviidae) in Bolivia : trends toward domesticity and possibleinfection with Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) Noireau, François Bosseno, Marie-France Carrasco, Roxana Telleria, Jenny Vargas, Fernando Camacho, Clara Yaksic, Nina Brenière, Simone Frederique ERATYRUS MUCRONATUS TRIATOMA SÓRDIDA TRIATOMINOS SELVÁTICOS TRYPANOSOMA CRUZI BOLIVIA ABSTRACT The risk of domestic transmission of Trypanosoma cruzi (Chagas) by sylvatic triatomines was assessed in an isolated area of the subandean region of Bolivia. None of the 390 residents examined had serological evidence of infection. Two sylvatic triatomine species, Eratyrus mucronatus (Stal) and Triatoma sordida (Stal), were found in houses and in peridomestic structures. The collection of nymphal instars of both species from some houses indicated possible domesticity. Microscopic examination of feces from 92 insects showed no parasites, and cultures from the guts of 30 insects were negative. Nevertheless, a polymerase chain reaction (PCR) test performed on the same fecal samples showed the presence of T. cruzi DNA in 19.1 and 12.5% of E. mucronatus and T. sordida, respectively. These 16 PCR positive samples were hybridized with 2 T. cruzi-specific probes known from the domestic cycle in Bolivia (clones 20 and 39). At least 1 of these clones was identified in 7 bugs (5 E. mucronatus and 2 T sordida). Moreover, no hybridization was observed with these probes in 8 E. mucronatus and 1 T sordida samples that showed an amplified band by PCR. These data indicated that T. cruzi clones, genetically unrelated to clones 20 and 39, also were circulating in this area. Based on these results, the 2 sylvatic triatomine species encountered in Apolo should not be overlooked as possible local vectors of T cruzi. 2022-04-01T13:35:42Z 2022-04-01T13:35:42Z 1995 Article http://repositorio.umsa.bo/xmlui/handle/123456789/27685 es application/pdf Journal of Medical Entomology |
institution |
UMSA BO |
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country |
Bolivia |
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BO |
component |
Bibliográfico |
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biblioteca |
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America del Sur |
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Sistema de Unidades de Información de UMSA |
language |
Spanish / Castilian |
topic |
ERATYRUS MUCRONATUS TRIATOMA SÓRDIDA TRIATOMINOS SELVÁTICOS TRYPANOSOMA CRUZI BOLIVIA ERATYRUS MUCRONATUS TRIATOMA SÓRDIDA TRIATOMINOS SELVÁTICOS TRYPANOSOMA CRUZI BOLIVIA |
spellingShingle |
ERATYRUS MUCRONATUS TRIATOMA SÓRDIDA TRIATOMINOS SELVÁTICOS TRYPANOSOMA CRUZI BOLIVIA ERATYRUS MUCRONATUS TRIATOMA SÓRDIDA TRIATOMINOS SELVÁTICOS TRYPANOSOMA CRUZI BOLIVIA Noireau, François Bosseno, Marie-France Carrasco, Roxana Telleria, Jenny Vargas, Fernando Camacho, Clara Yaksic, Nina Brenière, Simone Frederique Sylvatic triatomines (Hemiptera: Reduviidae) in Bolivia : trends toward domesticity and possibleinfection with Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) |
description |
ABSTRACT
The risk of domestic transmission of Trypanosoma cruzi (Chagas) by sylvatic triatomines was assessed in an isolated area of the subandean region of Bolivia. None of the 390 residents examined had serological evidence of infection. Two sylvatic triatomine species, Eratyrus mucronatus (Stal) and Triatoma sordida (Stal), were found in houses and in peridomestic structures. The collection of nymphal instars of both species from some houses indicated possible domesticity. Microscopic examination of feces from 92 insects showed no parasites, and cultures from the guts of 30 insects were negative. Nevertheless, a polymerase chain reaction (PCR) test performed on the same fecal samples showed the presence of T. cruzi DNA in 19.1 and 12.5% of E. mucronatus and T. sordida, respectively. These 16 PCR positive samples were hybridized with 2 T. cruzi-specific probes known from the domestic cycle in Bolivia (clones 20 and 39). At least 1 of these clones was identified in 7 bugs (5 E. mucronatus and 2 T sordida). Moreover, no hybridization was observed with these probes in 8 E. mucronatus and 1 T sordida samples that showed an amplified band by PCR. These data indicated that T. cruzi clones, genetically unrelated to clones 20 and 39, also were circulating in this area. Based on these results, the 2 sylvatic triatomine species encountered in Apolo should not be overlooked as possible local vectors of T cruzi. |
format |
Article |
topic_facet |
ERATYRUS MUCRONATUS TRIATOMA SÓRDIDA TRIATOMINOS SELVÁTICOS TRYPANOSOMA CRUZI BOLIVIA |
author |
Noireau, François Bosseno, Marie-France Carrasco, Roxana Telleria, Jenny Vargas, Fernando Camacho, Clara Yaksic, Nina Brenière, Simone Frederique |
author_facet |
Noireau, François Bosseno, Marie-France Carrasco, Roxana Telleria, Jenny Vargas, Fernando Camacho, Clara Yaksic, Nina Brenière, Simone Frederique |
author_sort |
Noireau, François |
title |
Sylvatic triatomines (Hemiptera: Reduviidae) in Bolivia : trends toward domesticity and possibleinfection with Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) |
title_short |
Sylvatic triatomines (Hemiptera: Reduviidae) in Bolivia : trends toward domesticity and possibleinfection with Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) |
title_full |
Sylvatic triatomines (Hemiptera: Reduviidae) in Bolivia : trends toward domesticity and possibleinfection with Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) |
title_fullStr |
Sylvatic triatomines (Hemiptera: Reduviidae) in Bolivia : trends toward domesticity and possibleinfection with Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) |
title_full_unstemmed |
Sylvatic triatomines (Hemiptera: Reduviidae) in Bolivia : trends toward domesticity and possibleinfection with Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) |
title_sort |
sylvatic triatomines (hemiptera: reduviidae) in bolivia : trends toward domesticity and possibleinfection with trypanosoma cruzi (kinetoplastida: trypanosomatidae) |
publisher |
Journal of Medical Entomology |
publishDate |
1995 |
url |
http://repositorio.umsa.bo/xmlui/handle/123456789/27685 |
work_keys_str_mv |
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