Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: tempoal and regional trends
Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovis spoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism.
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Fundacao Oswaldo Cruz, Brasil
2014-04
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Subjects: | Mycobacterium bovis, Tuberculosis, Ganado Bovino, Tendencias, Genotipos, Buenos Aires, Cordoba (Argentina), Santa Fe (Argentina), Cattle, Trends, Genotypes, |
Online Access: | http://hdl.handle.net/20.500.12123/8221 https://memorias.ioc.fiocruz.br/article/1641/0292_genotyping-mycobacterium-bovis-from-cattle-in-the-central-pampas-of-argentina-temporal-and-regional-trends http://dx.doi.org/10.1590/0074-0276140292 |
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oai:localhost:20.500.12123-82212020-11-09T17:19:06Z Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: tempoal and regional trends Shimizu, Ernesto Macias, Analía Paolicchi, Fernando Magnano, Gabriel Zapata, Laura Fernandez, Analía Canal, Ana María Garbaccio, Sergio Gabriel Cataldi, Angel Adrian Caimi, Karina Cynthia Zumarraga, Martin Jose Mycobacterium bovis Tuberculosis Ganado Bovino Tendencias Genotipos Buenos Aires Cordoba (Argentina) Santa Fe (Argentina) Cattle Trends Genotypes Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovis spoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism. Instituto de Patobiología Fil: Shimizu, Ernesto. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Macias, Analía. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Paolicchi, Fernando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Magnano, Gabriel. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Zapata, Laura. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Fernandez, Analía. Ministerio de la Producción. Dirección General de Ganadería y Avicultura. Laboratorio de Sanidad Animal; Argentina Fil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Patología Básica; Argentina Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2020-11-09T16:59:02Z 2020-11-09T16:59:02Z 2014-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8221 https://memorias.ioc.fiocruz.br/article/1641/0292_genotyping-mycobacterium-bovis-from-cattle-in-the-central-pampas-of-argentina-temporal-and-regional-trends 1678-8060 0074-0276 http://dx.doi.org/10.1590/0074-0276140292 eng info:eu-repo/semantics/openAccess application/pdf Fundacao Oswaldo Cruz, Brasil Memorias do Instituto Oswaldo Cruz 109 (2) : 236-245 (Abril 2014) |
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Mycobacterium bovis Tuberculosis Ganado Bovino Tendencias Genotipos Buenos Aires Cordoba (Argentina) Santa Fe (Argentina) Cattle Trends Genotypes Mycobacterium bovis Tuberculosis Ganado Bovino Tendencias Genotipos Buenos Aires Cordoba (Argentina) Santa Fe (Argentina) Cattle Trends Genotypes |
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Mycobacterium bovis Tuberculosis Ganado Bovino Tendencias Genotipos Buenos Aires Cordoba (Argentina) Santa Fe (Argentina) Cattle Trends Genotypes Mycobacterium bovis Tuberculosis Ganado Bovino Tendencias Genotipos Buenos Aires Cordoba (Argentina) Santa Fe (Argentina) Cattle Trends Genotypes Shimizu, Ernesto Macias, Analía Paolicchi, Fernando Magnano, Gabriel Zapata, Laura Fernandez, Analía Canal, Ana María Garbaccio, Sergio Gabriel Cataldi, Angel Adrian Caimi, Karina Cynthia Zumarraga, Martin Jose Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: tempoal and regional trends |
description |
Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovis spoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism. |
format |
info:ar-repo/semantics/artículo |
topic_facet |
Mycobacterium bovis Tuberculosis Ganado Bovino Tendencias Genotipos Buenos Aires Cordoba (Argentina) Santa Fe (Argentina) Cattle Trends Genotypes |
author |
Shimizu, Ernesto Macias, Analía Paolicchi, Fernando Magnano, Gabriel Zapata, Laura Fernandez, Analía Canal, Ana María Garbaccio, Sergio Gabriel Cataldi, Angel Adrian Caimi, Karina Cynthia Zumarraga, Martin Jose |
author_facet |
Shimizu, Ernesto Macias, Analía Paolicchi, Fernando Magnano, Gabriel Zapata, Laura Fernandez, Analía Canal, Ana María Garbaccio, Sergio Gabriel Cataldi, Angel Adrian Caimi, Karina Cynthia Zumarraga, Martin Jose |
author_sort |
Shimizu, Ernesto |
title |
Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: tempoal and regional trends |
title_short |
Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: tempoal and regional trends |
title_full |
Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: tempoal and regional trends |
title_fullStr |
Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: tempoal and regional trends |
title_full_unstemmed |
Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: tempoal and regional trends |
title_sort |
genotyping mycobacterium bovis from cattle in the central pampas of argentina: tempoal and regional trends |
publisher |
Fundacao Oswaldo Cruz, Brasil |
publishDate |
2014-04 |
url |
http://hdl.handle.net/20.500.12123/8221 https://memorias.ioc.fiocruz.br/article/1641/0292_genotyping-mycobacterium-bovis-from-cattle-in-the-central-pampas-of-argentina-temporal-and-regional-trends http://dx.doi.org/10.1590/0074-0276140292 |
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