First Report of Tobacco Streak Virus Infecting Sunflower in Argentina
Sunflower (Helianthus annuus) is one of the main oleaginous crops in the world, and Argentina is positioned among the top sunflower seed producers worldwide (FAO 2019). During the 2018 to 2019 crop season, sunflower plants showing symptoms similar to those reported for the tobacco streak virus (TSV) were observed in one field of Rivera, Buenos Aires province. The symptoms included leaf mosaic and severe necrosis of stem, petioles, leaves, and pith, often progressing to complete collapse and death of affected plants. Samples from symptomatic leaf tissue were collected from five plants, and leaves of sunflower seedlings maintained under greenhouse conditions were used as the healthy control. Leaf extracts obtained from symptomatic leaves were positive by double antibody sandwich ELISA test, using a polyclonal antibody against TSV (Bioreba, Reinach, Switzerland). The average optical density of infected samples was 0.384, eight times higher than the mean plus three standard deviations of four healthy controls. Total RNA was extracted from collected samples using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription polymerase chain reaction (RT-PCR) with specific TSV primers (Almeida et al. 2005). Only infected samples produced expected amplicons (∼1 kb). The RT-PCR products obtained in duplicate from two symptomatic plants were purified and sequenced in both directions
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Format: | info:ar-repo/semantics/artículo biblioteca |
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American Phytopathological Society
2019-11-21
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Subjects: | Helianthus Annuus, Virus de las Plantas, Argentina, Plant Viruses, Tobacco Streak Virus, Sunflower, Girasol, |
Online Access: | https://apsjournals.apsnet.org/doi/10.1094/PDIS-04-19-0849-PDN http://hdl.handle.net/20.500.12123/8096 https://doi.org/10.1094/PDIS-04-19-0849-PDN |
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oai:localhost:20.500.12123-80962020-10-21T16:49:21Z First Report of Tobacco Streak Virus Infecting Sunflower in Argentina Cabrera Mederos, Dariel Trucco, Veronica Milagros Bejerman, Nicolas Esteban Lenardon, Sergio Luis Giolitti, Fabian Helianthus Annuus Virus de las Plantas Argentina Plant Viruses Tobacco Streak Virus Sunflower Girasol Sunflower (Helianthus annuus) is one of the main oleaginous crops in the world, and Argentina is positioned among the top sunflower seed producers worldwide (FAO 2019). During the 2018 to 2019 crop season, sunflower plants showing symptoms similar to those reported for the tobacco streak virus (TSV) were observed in one field of Rivera, Buenos Aires province. The symptoms included leaf mosaic and severe necrosis of stem, petioles, leaves, and pith, often progressing to complete collapse and death of affected plants. Samples from symptomatic leaf tissue were collected from five plants, and leaves of sunflower seedlings maintained under greenhouse conditions were used as the healthy control. Leaf extracts obtained from symptomatic leaves were positive by double antibody sandwich ELISA test, using a polyclonal antibody against TSV (Bioreba, Reinach, Switzerland). The average optical density of infected samples was 0.384, eight times higher than the mean plus three standard deviations of four healthy controls. Total RNA was extracted from collected samples using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription polymerase chain reaction (RT-PCR) with specific TSV primers (Almeida et al. 2005). Only infected samples produced expected amplicons (∼1 kb). The RT-PCR products obtained in duplicate from two symptomatic plants were purified and sequenced in both directions Instituto de Patología Vegetal Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina Fil: Trucco, Verónica Milagros. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina 2020-10-21T16:41:30Z 2020-10-21T16:41:30Z 2019-11-21 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://apsjournals.apsnet.org/doi/10.1094/PDIS-04-19-0849-PDN http://hdl.handle.net/20.500.12123/8096 0191-2917 1943-7692 https://doi.org/10.1094/PDIS-04-19-0849-PDN eng info:eu-repo/semantics/openAccess application/pdf American Phytopathological Society Plant Disease 103 (12) : 3290 (December 2019) |
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Helianthus Annuus Virus de las Plantas Argentina Plant Viruses Tobacco Streak Virus Sunflower Girasol Helianthus Annuus Virus de las Plantas Argentina Plant Viruses Tobacco Streak Virus Sunflower Girasol |
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Helianthus Annuus Virus de las Plantas Argentina Plant Viruses Tobacco Streak Virus Sunflower Girasol Helianthus Annuus Virus de las Plantas Argentina Plant Viruses Tobacco Streak Virus Sunflower Girasol Cabrera Mederos, Dariel Trucco, Veronica Milagros Bejerman, Nicolas Esteban Lenardon, Sergio Luis Giolitti, Fabian First Report of Tobacco Streak Virus Infecting Sunflower in Argentina |
description |
Sunflower (Helianthus annuus) is one of the main oleaginous crops in the world, and Argentina is positioned among the top sunflower seed producers worldwide (FAO 2019). During the 2018 to 2019 crop season, sunflower plants showing symptoms similar to those reported for the tobacco streak virus (TSV) were observed in one field of Rivera, Buenos Aires province. The symptoms included leaf mosaic and severe necrosis of stem, petioles, leaves, and pith, often progressing to complete collapse and death of affected plants. Samples from symptomatic leaf tissue were collected from five plants, and leaves of sunflower seedlings maintained under greenhouse conditions were used as the healthy control. Leaf extracts obtained from symptomatic leaves were positive by double antibody sandwich ELISA test, using a polyclonal antibody against TSV (Bioreba, Reinach, Switzerland). The average optical density of infected samples was 0.384, eight times higher than the mean plus three standard deviations of four healthy controls. Total RNA was extracted from collected samples using an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription polymerase chain reaction (RT-PCR) with specific TSV primers (Almeida et al. 2005). Only infected samples produced expected amplicons (∼1 kb). The RT-PCR products obtained in duplicate from two symptomatic plants were purified and sequenced in both directions |
format |
info:ar-repo/semantics/artículo |
topic_facet |
Helianthus Annuus Virus de las Plantas Argentina Plant Viruses Tobacco Streak Virus Sunflower Girasol |
author |
Cabrera Mederos, Dariel Trucco, Veronica Milagros Bejerman, Nicolas Esteban Lenardon, Sergio Luis Giolitti, Fabian |
author_facet |
Cabrera Mederos, Dariel Trucco, Veronica Milagros Bejerman, Nicolas Esteban Lenardon, Sergio Luis Giolitti, Fabian |
author_sort |
Cabrera Mederos, Dariel |
title |
First Report of Tobacco Streak Virus Infecting Sunflower in Argentina |
title_short |
First Report of Tobacco Streak Virus Infecting Sunflower in Argentina |
title_full |
First Report of Tobacco Streak Virus Infecting Sunflower in Argentina |
title_fullStr |
First Report of Tobacco Streak Virus Infecting Sunflower in Argentina |
title_full_unstemmed |
First Report of Tobacco Streak Virus Infecting Sunflower in Argentina |
title_sort |
first report of tobacco streak virus infecting sunflower in argentina |
publisher |
American Phytopathological Society |
publishDate |
2019-11-21 |
url |
https://apsjournals.apsnet.org/doi/10.1094/PDIS-04-19-0849-PDN http://hdl.handle.net/20.500.12123/8096 https://doi.org/10.1094/PDIS-04-19-0849-PDN |
work_keys_str_mv |
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