Molecular characterization of Cryptosporidium spp. from domestic pigs in Argentina
Cryptosporidiosis in pigs is caused by different Cryptosporidium species or genotypes, with C. suis and C. scrofarum considered porcine specific species. There is scarce information on Cryptosporidium infection in pigs in South America. A total of 520 individual faecal samples were obtained from 1, 2, 3 and 4 week old piglets (n = 130 from each age group), from 13 Argentinean intensive pig farms. The diagnosis of species of Cryptosporidium combined microscopy and molecular techniques. Genotyping from samples with Cryptosporidium oocysts at microscopy was performed by genus-specific and species-specific nested PCR targeting 18S rRNA gene fragments, and sequencing. Microscopic analysis detected Cryptosporidium oocysts in 47/520 (9%) faecal samples from 11/13 (85%) farms, with farm infection rates between 0 and 17.5%. Presence of Cryptosporidium oocysts was associated with diarrhea. The proportion of microscopically positive samples was not associated with piglet age. A total of 15/47 (32% of samples with oocyst compatible structures) were positive by genus and species-specific nested PCR. Species-specific PCR and sequencing showed presence of C. suis, C. scrofarum, and both species in 3, 8 and 4 samples, respectively. The proportion of positive samples on each specific PCR was similar between age groups, being C. suis proportion slightly higher in 4 week old piglets. The use of molecular tools allowed the confirmation of C. suis and C. scrofarum infection in Argentinean pigs. Cryptosporidiosis was widely distributed in the main pig husbandry area from Argentina, with a low to moderate intra farm infection rate.
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Elsevier
2020-09
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Subjects: | Cerdo, Enfermedades de los Animales, Cryptosporidium, Oocistos, Lechón, Swine, Animal Diseases, Oocysts, Piglets, Argentina, Cryptosporidium suis, Cryptosporidium scrofarum, |
Online Access: | http://hdl.handle.net/20.500.12123/7991 https://www.sciencedirect.com/science/article/abs/pii/S2405939020302549 https://doi.org/10.1016/j.vprsr.2020.100473 |
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oai:localhost:20.500.12123-79912023-02-24T16:23:46Z Molecular characterization of Cryptosporidium spp. from domestic pigs in Argentina de Felice, Lorena Alejandra Moré, Gastón Cappuccio, Javier Alejandro Venturini, María Cecilia Unzaga, Juan Manuel Cerdo Enfermedades de los Animales Cryptosporidium Oocistos Lechón Swine Animal Diseases Oocysts Piglets Argentina Cryptosporidium suis Cryptosporidium scrofarum Cryptosporidiosis in pigs is caused by different Cryptosporidium species or genotypes, with C. suis and C. scrofarum considered porcine specific species. There is scarce information on Cryptosporidium infection in pigs in South America. A total of 520 individual faecal samples were obtained from 1, 2, 3 and 4 week old piglets (n = 130 from each age group), from 13 Argentinean intensive pig farms. The diagnosis of species of Cryptosporidium combined microscopy and molecular techniques. Genotyping from samples with Cryptosporidium oocysts at microscopy was performed by genus-specific and species-specific nested PCR targeting 18S rRNA gene fragments, and sequencing. Microscopic analysis detected Cryptosporidium oocysts in 47/520 (9%) faecal samples from 11/13 (85%) farms, with farm infection rates between 0 and 17.5%. Presence of Cryptosporidium oocysts was associated with diarrhea. The proportion of microscopically positive samples was not associated with piglet age. A total of 15/47 (32% of samples with oocyst compatible structures) were positive by genus and species-specific nested PCR. Species-specific PCR and sequencing showed presence of C. suis, C. scrofarum, and both species in 3, 8 and 4 samples, respectively. The proportion of positive samples on each specific PCR was similar between age groups, being C. suis proportion slightly higher in 4 week old piglets. The use of molecular tools allowed the confirmation of C. suis and C. scrofarum infection in Argentinean pigs. Cryptosporidiosis was widely distributed in the main pig husbandry area from Argentina, with a low to moderate intra farm infection rate. EEA Marcos Juárez Fil: de Felice, Lorena A. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina Fil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cappuccio, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina Fil: Unzaga, Juan Manuel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Laboratorio de Inmunoparasitología; Argentina 2020-09-30T13:13:54Z 2020-09-30T13:13:54Z 2020-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7991 https://www.sciencedirect.com/science/article/abs/pii/S2405939020302549 2405-9390 https://doi.org/10.1016/j.vprsr.2020.100473 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Parasitology: Regional Studies and Reports 22 : 100473 (December 2020) |
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Cerdo Enfermedades de los Animales Cryptosporidium Oocistos Lechón Swine Animal Diseases Oocysts Piglets Argentina Cryptosporidium suis Cryptosporidium scrofarum Cerdo Enfermedades de los Animales Cryptosporidium Oocistos Lechón Swine Animal Diseases Oocysts Piglets Argentina Cryptosporidium suis Cryptosporidium scrofarum |
spellingShingle |
Cerdo Enfermedades de los Animales Cryptosporidium Oocistos Lechón Swine Animal Diseases Oocysts Piglets Argentina Cryptosporidium suis Cryptosporidium scrofarum Cerdo Enfermedades de los Animales Cryptosporidium Oocistos Lechón Swine Animal Diseases Oocysts Piglets Argentina Cryptosporidium suis Cryptosporidium scrofarum de Felice, Lorena Alejandra Moré, Gastón Cappuccio, Javier Alejandro Venturini, María Cecilia Unzaga, Juan Manuel Molecular characterization of Cryptosporidium spp. from domestic pigs in Argentina |
description |
Cryptosporidiosis in pigs is caused by different Cryptosporidium species or genotypes, with C. suis and C. scrofarum considered porcine specific species. There is scarce information on Cryptosporidium infection in pigs in South America. A total of 520 individual faecal samples were obtained from 1, 2, 3 and 4 week old piglets (n = 130 from each age group), from 13 Argentinean intensive pig farms. The diagnosis of species of Cryptosporidium combined microscopy and molecular techniques. Genotyping from samples with Cryptosporidium oocysts at microscopy was performed by genus-specific and species-specific nested PCR targeting 18S rRNA gene fragments, and sequencing. Microscopic analysis detected Cryptosporidium oocysts in 47/520 (9%) faecal samples from 11/13 (85%) farms, with farm infection rates between 0 and 17.5%. Presence of Cryptosporidium oocysts was associated with diarrhea. The proportion of microscopically positive samples was not associated with piglet age. A total of 15/47 (32% of samples with oocyst compatible structures) were positive by genus and species-specific nested PCR. Species-specific PCR and sequencing showed presence of C. suis, C. scrofarum, and both species in 3, 8 and 4 samples, respectively. The proportion of positive samples on each specific PCR was similar between age groups, being C. suis proportion slightly higher in 4 week old piglets. The use of molecular tools allowed the confirmation of C. suis and C. scrofarum infection in Argentinean pigs. Cryptosporidiosis was widely distributed in the main pig husbandry area from Argentina, with a low to moderate intra farm infection rate. |
format |
info:ar-repo/semantics/artículo |
topic_facet |
Cerdo Enfermedades de los Animales Cryptosporidium Oocistos Lechón Swine Animal Diseases Oocysts Piglets Argentina Cryptosporidium suis Cryptosporidium scrofarum |
author |
de Felice, Lorena Alejandra Moré, Gastón Cappuccio, Javier Alejandro Venturini, María Cecilia Unzaga, Juan Manuel |
author_facet |
de Felice, Lorena Alejandra Moré, Gastón Cappuccio, Javier Alejandro Venturini, María Cecilia Unzaga, Juan Manuel |
author_sort |
de Felice, Lorena Alejandra |
title |
Molecular characterization of Cryptosporidium spp. from domestic pigs in Argentina |
title_short |
Molecular characterization of Cryptosporidium spp. from domestic pigs in Argentina |
title_full |
Molecular characterization of Cryptosporidium spp. from domestic pigs in Argentina |
title_fullStr |
Molecular characterization of Cryptosporidium spp. from domestic pigs in Argentina |
title_full_unstemmed |
Molecular characterization of Cryptosporidium spp. from domestic pigs in Argentina |
title_sort |
molecular characterization of cryptosporidium spp. from domestic pigs in argentina |
publisher |
Elsevier |
publishDate |
2020-09 |
url |
http://hdl.handle.net/20.500.12123/7991 https://www.sciencedirect.com/science/article/abs/pii/S2405939020302549 https://doi.org/10.1016/j.vprsr.2020.100473 |
work_keys_str_mv |
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_version_ |
1763177000327970816 |