Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)
A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome.
| Auteurs principaux: | , , , |
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| Format: | info:ar-repo/semantics/artículo biblioteca |
| Langue: | eng |
| Publié: |
Springer; Virology Division, International Union of Microbiological Societies
2013-02
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| Sujets: | Helianthus Annuus, Virus de las Plantas, Clones, Potyvirus, Inoculación, Plant Viruses, Potyviruses, Inoculation, Girasol, Virus del Moteado Clorótico del Girasol, Sunflower, SuCMoV, Sunflower Chlorotic Mottle Virus, Infectious Clone, |
| Accès en ligne: | http://hdl.handle.net/20.500.12123/3711 https://link.springer.com/article/10.1007/s00705-012-1497-2 |
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