Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)
A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome.
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Format: | info:ar-repo/semantics/artículo biblioteca |
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Springer; Virology Division, International Union of Microbiological Societies
2013-02
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Subjects: | Helianthus Annuus, Virus de las Plantas, Clones, Potyvirus, Inoculación, Plant Viruses, Potyviruses, Inoculation, Girasol, Virus del Moteado Clorótico del Girasol, Sunflower, SuCMoV, Sunflower Chlorotic Mottle Virus, Infectious Clone, |
Online Access: | http://hdl.handle.net/20.500.12123/3711 https://link.springer.com/article/10.1007/s00705-012-1497-2 |
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oai:localhost:20.500.12123-37112018-10-25T15:30:19Z Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV) Bejerman, Nicolas Esteban Giolitti, Fabian De Breuil, Soledad Lenardon, Sergio Luis Helianthus Annuus Virus de las Plantas Clones Potyvirus Inoculación Plant Viruses Potyviruses Inoculation Girasol Virus del Moteado Clorótico del Girasol Sunflower SuCMoV Sunflower Chlorotic Mottle Virus Infectious Clone A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome. Instituto de Patología Vegetal Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina 2018-10-25T15:25:00Z 2018-10-25T15:25:00Z 2013-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3711 https://link.springer.com/article/10.1007/s00705-012-1497-2 0304-8608 1432-8798 (Online) 10.1007/s00705-012-1497-2 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer; Virology Division, International Union of Microbiological Societies Archives of virology 158 (2) : 485–490. (February 2013) |
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Helianthus Annuus Virus de las Plantas Clones Potyvirus Inoculación Plant Viruses Potyviruses Inoculation Girasol Virus del Moteado Clorótico del Girasol Sunflower SuCMoV Sunflower Chlorotic Mottle Virus Infectious Clone Helianthus Annuus Virus de las Plantas Clones Potyvirus Inoculación Plant Viruses Potyviruses Inoculation Girasol Virus del Moteado Clorótico del Girasol Sunflower SuCMoV Sunflower Chlorotic Mottle Virus Infectious Clone |
spellingShingle |
Helianthus Annuus Virus de las Plantas Clones Potyvirus Inoculación Plant Viruses Potyviruses Inoculation Girasol Virus del Moteado Clorótico del Girasol Sunflower SuCMoV Sunflower Chlorotic Mottle Virus Infectious Clone Helianthus Annuus Virus de las Plantas Clones Potyvirus Inoculación Plant Viruses Potyviruses Inoculation Girasol Virus del Moteado Clorótico del Girasol Sunflower SuCMoV Sunflower Chlorotic Mottle Virus Infectious Clone Bejerman, Nicolas Esteban Giolitti, Fabian De Breuil, Soledad Lenardon, Sergio Luis Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV) |
description |
A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome. |
format |
info:ar-repo/semantics/artículo |
topic_facet |
Helianthus Annuus Virus de las Plantas Clones Potyvirus Inoculación Plant Viruses Potyviruses Inoculation Girasol Virus del Moteado Clorótico del Girasol Sunflower SuCMoV Sunflower Chlorotic Mottle Virus Infectious Clone |
author |
Bejerman, Nicolas Esteban Giolitti, Fabian De Breuil, Soledad Lenardon, Sergio Luis |
author_facet |
Bejerman, Nicolas Esteban Giolitti, Fabian De Breuil, Soledad Lenardon, Sergio Luis |
author_sort |
Bejerman, Nicolas Esteban |
title |
Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV) |
title_short |
Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV) |
title_full |
Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV) |
title_fullStr |
Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV) |
title_full_unstemmed |
Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV) |
title_sort |
development of a full-length infectious clone of sunflower chlorotic mottle virus (sucmov) |
publisher |
Springer; Virology Division, International Union of Microbiological Societies |
publishDate |
2013-02 |
url |
http://hdl.handle.net/20.500.12123/3711 https://link.springer.com/article/10.1007/s00705-012-1497-2 |
work_keys_str_mv |
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_version_ |
1756007290772652033 |