Genetic diversity in a world germplasm collection of tall fescue

Festuca arundinacea Schreb., commonly known as tall fescue, is a major forage crop in temperate regions. Recently, a molecular analysis of different accessions of a world germplasm collection of tall fescue has demonstrated that it contains different species from the genus Festuca and allowed their rapid classification into the three major morphotypes (Continental, Mediterranean and Rhizomatous). In this study, we explored the genetic diversity of 161 accessions of Festuca species from 29 countries, including 28 accessions of INTA (Argentina), by analyzing 15 polymorphic SSR markers by capillary electrophoresis. These molecular markers allowed us to detect a total of 214 alleles. The number of alleles per locus varied between 5 and 24, and the values of polymorphic information content ranged from 0.627 to 0.840. In addition, the accessions analyzed by flow cytometry showed different ploidy levels (diploid, tetraploid, hexaploid and octaploid), placing in evidence that the world germplasm collection consisted of multiple species, as previously suggested. Interestingly, almost all accessions of INTA germplasm collection were true hexaploid tall fescue, belonging to two eco-geographic races (Continental and Mediterranean). Finally, the data presented revealed an ample genetic diversity of tall fescue showing the importance of preserving the INTA collection for future breeding programs

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Bibliographic Details
Main Authors: Cuyeu, Alba Romina, Rosso, Beatriz Susana, Pagano, Elba Maria, Soto, Gabriela, Fox, Ana Romina, Ayub, Nicolás Daniel
Format: info:ar-repo/semantics/artículo biblioteca
Language:eng
Published: 2013
Subjects:Festuca Arundinacea, Germoplasma, Variación Genética, Marcadores Genéticos, Germplasm, Genetic Variation, Genetic Markers, Festuca Alta, Diversidad Genética,
Online Access:http://hdl.handle.net/20.500.12123/1371
http://www.scielo.br/pdf/gmb/v36n2/2012-335.pdf
https://inta.gob.ar/sites/default/files/inta-cuyeugeneticdiversityfescue2013.pdf
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spelling oai:localhost:20.500.12123-13712017-12-27T15:51:15Z Genetic diversity in a world germplasm collection of tall fescue Cuyeu, Alba Romina Rosso, Beatriz Susana Pagano, Elba Maria Soto, Gabriela Fox, Ana Romina Ayub, Nicolás Daniel Festuca Arundinacea Germoplasma Variación Genética Marcadores Genéticos Germplasm Genetic Variation Genetic Markers Festuca Alta Diversidad Genética Festuca arundinacea Schreb., commonly known as tall fescue, is a major forage crop in temperate regions. Recently, a molecular analysis of different accessions of a world germplasm collection of tall fescue has demonstrated that it contains different species from the genus Festuca and allowed their rapid classification into the three major morphotypes (Continental, Mediterranean and Rhizomatous). In this study, we explored the genetic diversity of 161 accessions of Festuca species from 29 countries, including 28 accessions of INTA (Argentina), by analyzing 15 polymorphic SSR markers by capillary electrophoresis. These molecular markers allowed us to detect a total of 214 alleles. The number of alleles per locus varied between 5 and 24, and the values of polymorphic information content ranged from 0.627 to 0.840. In addition, the accessions analyzed by flow cytometry showed different ploidy levels (diploid, tetraploid, hexaploid and octaploid), placing in evidence that the world germplasm collection consisted of multiple species, as previously suggested. Interestingly, almost all accessions of INTA germplasm collection were true hexaploid tall fescue, belonging to two eco-geographic races (Continental and Mediterranean). Finally, the data presented revealed an ample genetic diversity of tall fescue showing the importance of preserving the INTA collection for future breeding programs Fil: Cuyeu, Alba Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Rosso, Beatriz Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina Fil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Soto, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fox, Ana Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-10-02T13:18:36Z 2017-10-02T13:18:36Z 2013 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1371 http://www.scielo.br/pdf/gmb/v36n2/2012-335.pdf https://inta.gob.ar/sites/default/files/inta-cuyeugeneticdiversityfescue2013.pdf 1415-4757 (Print) 1678-4685 (Online) eng info:eu-repo/semantics/openAccess application/pdf Genetics and molecular biology 36 (2) : 237-242.(2013)
institution INTA AR
collection DSpace
country Argentina
countrycode AR
component Bibliográfico
access En linea
databasecode dig-inta-ar
tag biblioteca
region America del Sur
libraryname Biblioteca Central del INTA Argentina
language eng
topic Festuca Arundinacea
Germoplasma
Variación Genética
Marcadores Genéticos
Germplasm
Genetic Variation
Genetic Markers
Festuca Alta
Diversidad Genética
Festuca Arundinacea
Germoplasma
Variación Genética
Marcadores Genéticos
Germplasm
Genetic Variation
Genetic Markers
Festuca Alta
Diversidad Genética
spellingShingle Festuca Arundinacea
Germoplasma
Variación Genética
Marcadores Genéticos
Germplasm
Genetic Variation
Genetic Markers
Festuca Alta
Diversidad Genética
Festuca Arundinacea
Germoplasma
Variación Genética
Marcadores Genéticos
Germplasm
Genetic Variation
Genetic Markers
Festuca Alta
Diversidad Genética
Cuyeu, Alba Romina
Rosso, Beatriz Susana
Pagano, Elba Maria
Soto, Gabriela
Fox, Ana Romina
Ayub, Nicolás Daniel
Genetic diversity in a world germplasm collection of tall fescue
description Festuca arundinacea Schreb., commonly known as tall fescue, is a major forage crop in temperate regions. Recently, a molecular analysis of different accessions of a world germplasm collection of tall fescue has demonstrated that it contains different species from the genus Festuca and allowed their rapid classification into the three major morphotypes (Continental, Mediterranean and Rhizomatous). In this study, we explored the genetic diversity of 161 accessions of Festuca species from 29 countries, including 28 accessions of INTA (Argentina), by analyzing 15 polymorphic SSR markers by capillary electrophoresis. These molecular markers allowed us to detect a total of 214 alleles. The number of alleles per locus varied between 5 and 24, and the values of polymorphic information content ranged from 0.627 to 0.840. In addition, the accessions analyzed by flow cytometry showed different ploidy levels (diploid, tetraploid, hexaploid and octaploid), placing in evidence that the world germplasm collection consisted of multiple species, as previously suggested. Interestingly, almost all accessions of INTA germplasm collection were true hexaploid tall fescue, belonging to two eco-geographic races (Continental and Mediterranean). Finally, the data presented revealed an ample genetic diversity of tall fescue showing the importance of preserving the INTA collection for future breeding programs
format info:ar-repo/semantics/artículo
topic_facet Festuca Arundinacea
Germoplasma
Variación Genética
Marcadores Genéticos
Germplasm
Genetic Variation
Genetic Markers
Festuca Alta
Diversidad Genética
author Cuyeu, Alba Romina
Rosso, Beatriz Susana
Pagano, Elba Maria
Soto, Gabriela
Fox, Ana Romina
Ayub, Nicolás Daniel
author_facet Cuyeu, Alba Romina
Rosso, Beatriz Susana
Pagano, Elba Maria
Soto, Gabriela
Fox, Ana Romina
Ayub, Nicolás Daniel
author_sort Cuyeu, Alba Romina
title Genetic diversity in a world germplasm collection of tall fescue
title_short Genetic diversity in a world germplasm collection of tall fescue
title_full Genetic diversity in a world germplasm collection of tall fescue
title_fullStr Genetic diversity in a world germplasm collection of tall fescue
title_full_unstemmed Genetic diversity in a world germplasm collection of tall fescue
title_sort genetic diversity in a world germplasm collection of tall fescue
publishDate 2013
url http://hdl.handle.net/20.500.12123/1371
http://www.scielo.br/pdf/gmb/v36n2/2012-335.pdf
https://inta.gob.ar/sites/default/files/inta-cuyeugeneticdiversityfescue2013.pdf
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