Growth response of Saccharomyces cerevisiae strains to stressors associated to the vine cycle
Saccharomyces cerevisiae isolates from grapes, soil, vine bark and buds collected at seven phenological stages of an annual growth cycle, were molecular typed by Microsatellite Multiplex PCR. Subsequently 30 S. cerevisiae genotypes were selected and the effect of vineyard environmental stressors, in both sublethal upper and lower levels, on their growth parameters was evaluated. The effect of low and high temperature (7–40 ◦C), pH (2.5–8.0), glucose concentration (3.0–300.0 g/L), nitrogen concentration (0.008–8.0 g/L), and copper presence (24 mg/L) were modelled individually using the reparametrized Gompertz equation. Multivariate ANOVA and Generalized Procrustes Analysis were used to determine the environmental stressor’s influence over the lag phase (λ) and the maximum specific growth rate (μmax). Both parameters were significantly affected by the S. cerevisiae genotype, the treatments, and the interaction between them. Despite a generalized reduction in μmax and a variable answer in λ, the 30 S. cerevisiae genotypes were able to overcome all the treatments. Extreme glucose limitation, copper presence and low temperature had the highest impact over the growth parameters. Interestingly, ten genotypes mostly distributed in the vineyard were the least affected, suggesting a greater acclimatization fitness and the possibility to persist in the changing conditions of the vine annual cycle.
Main Authors: | Gonzalez, Magali Lucia Rosa, Valero, Eva, Chimeno, Selva Valeria, Garrido Fernandez, Antonio, Rodriguez Gomez, Francisco, Rojo, Cecilia, Paolinelli, Marcos, Arroyo Lopez, Francisco Noe, Combina, Mariana, Mercado, Laura Analia |
---|---|
Format: | info:ar-repo/semantics/artículo biblioteca |
Language: | eng |
Published: |
Elsevier
2022-03-07
|
Subjects: | Vid, Levadura, Identificación, Microsatélites, Saccharomyces cerevisiae, Grapevines, Yeasts, Identification, Microsatellites, |
Online Access: | http://hdl.handle.net/20.500.12123/11335 https://www.sciencedirect.com/science/article/pii/S0023643822000925 https://doi.org/10.1016/j.lwt.2022.113157 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Caracterización de los reservorios y la biodiversidad de levaduras Saccharomyces cerevisiae en un viñedo comercial durante un ciclo fenológico completo de la vid
by: Gonzalez, Magali Lucia Rosa, et al.
Published: (2021-10-25) -
Culture-dependent and independent techniques applied to monitor yeast species on cold soak at different temperatures in winemaking
by: Maturano, Yolanda Paola, et al.
Published: (2016) -
Selection of non-Saccharomyces yeasts to be used in grape musts with high alcoholic potential: A strategy to obtain wine with reduced etanol content
by: Mestre Furlani, Maria Victoria, et al.
Published: (2017) -
Biodiversity of Saccharomyces cerevisiae populations in Malbec vineyards from the “Zona Alta del Río Mendoza” region in Argentina
by: Mercado, Laura Analia, et al.
Published: (2011-12-15) -
Generation of a non-transgenic genetically improved yeast strain for wine production from nitrogen-deficient musts
by: Kessi Pérez, Eduardo Ignacio, et al.
Published: (2020)