First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina
Symptomatic sweet potato cv Arapey INIA samples were collected from a commercial production field in Colonia Molina, Guaymallén department, Mendoza province, Argentina. They showed dark rounded lesions, sometimes coalescing with white granular mycelium. Fungus was obtained from symptomatic sweet potatoes, which represented the generalized infection that affected the crop. They were seeded in PDA with streptomycin sulfate and incubated for seven days at 21°C, alternating white/black (UV400nm) light. Observations with an optical microscope revealed the presence of hyaline, not septated, cylindrical endoconidia with rounded ends. They were 8-16 μm length and 4–6 μm width. Phialides were 43-46 μm length, rounded bases (7-9 μm width) and tapering to the neck´s tip (4-6 μm width). Brown chlamydospores (aleuriospores), 9-13 μm length and 8-12 μm width, in chains of 2-8 spores were observed. For molecular identification, total genomic DNA was extracted. ITS fragment of 565 pb was amplified using ITS5/ITS4 primers and sequenced. The sequence indicated 99% identity with Berkeleyomyces basicola (synonymous: Thielaviopsis basicola). This was deposited in GenBank as (KX580957) (CBS: C430.74, Gen Bank accession number AF275482.1). This is the first report of B. basicola in sweet potato in Argentina, a potential threat to storage root yields.
Main Authors: | , , , , , , |
---|---|
Format: | info:ar-repo/semantics/artículo biblioteca |
Language: | eng |
Published: |
Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo
2021-07-07
|
Subjects: | Ipomoea Batatas, Thielaviopsis Basicola, Argentina, Podredumbre de la Raíz, Batata, Enfermedades de las Plantas, Root Rots, Fungal Diseases, Sweet Potatoes, Plant Diseases, Enfermedades Fungosas, Chalara Elegans, Enfermedad Fúngica en Batata, Sweet Potato Fungal Disease, |
Online Access: | http://hdl.handle.net/20.500.12123/10179 https://revistas.uncu.edu.ar/ojs/index.php/RFCA/article/view/4898 https://doi.org/10.48162/rev.39.027 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:localhost:20.500.12123-10179 |
---|---|
record_format |
koha |
institution |
INTA AR |
collection |
DSpace |
country |
Argentina |
countrycode |
AR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inta-ar |
tag |
biblioteca |
region |
America del Sur |
libraryname |
Biblioteca Central del INTA Argentina |
language |
eng |
topic |
Ipomoea Batatas Thielaviopsis Basicola Argentina Podredumbre de la Raíz Batata Enfermedades de las Plantas Root Rots Fungal Diseases Sweet Potatoes Plant Diseases Enfermedades Fungosas Chalara Elegans Enfermedad Fúngica en Batata Sweet Potato Fungal Disease Ipomoea Batatas Thielaviopsis Basicola Argentina Podredumbre de la Raíz Batata Enfermedades de las Plantas Root Rots Fungal Diseases Sweet Potatoes Plant Diseases Enfermedades Fungosas Chalara Elegans Enfermedad Fúngica en Batata Sweet Potato Fungal Disease |
spellingShingle |
Ipomoea Batatas Thielaviopsis Basicola Argentina Podredumbre de la Raíz Batata Enfermedades de las Plantas Root Rots Fungal Diseases Sweet Potatoes Plant Diseases Enfermedades Fungosas Chalara Elegans Enfermedad Fúngica en Batata Sweet Potato Fungal Disease Ipomoea Batatas Thielaviopsis Basicola Argentina Podredumbre de la Raíz Batata Enfermedades de las Plantas Root Rots Fungal Diseases Sweet Potatoes Plant Diseases Enfermedades Fungosas Chalara Elegans Enfermedad Fúngica en Batata Sweet Potato Fungal Disease Martino, Julia Andrea Di Feo, Liliana Del Valle Paccioretti, Mauro Andres Contardi, Clara Adriana Sanchez, Miguel Alejandro Ortega, Leandro Ismael Pastor, Silvina Estela First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina |
description |
Symptomatic sweet potato cv Arapey INIA samples were collected from a commercial production field in Colonia Molina, Guaymallén department, Mendoza province, Argentina. They showed dark rounded lesions, sometimes coalescing with white granular mycelium. Fungus was obtained from symptomatic sweet potatoes, which represented the generalized infection that affected the crop. They were seeded in PDA with streptomycin sulfate and incubated for seven days at 21°C, alternating white/black (UV400nm) light. Observations with an optical microscope revealed the presence of hyaline, not septated, cylindrical endoconidia with rounded ends. They were 8-16 μm length and 4–6 μm width. Phialides were 43-46 μm length, rounded bases (7-9 μm width) and tapering to the neck´s tip (4-6 μm width). Brown chlamydospores (aleuriospores), 9-13 μm length and 8-12 μm width, in chains of 2-8 spores were observed. For molecular identification, total genomic DNA was extracted. ITS fragment of 565 pb was amplified using ITS5/ITS4 primers and sequenced. The sequence indicated 99% identity with Berkeleyomyces basicola (synonymous: Thielaviopsis basicola). This was deposited in GenBank as (KX580957) (CBS: C430.74, Gen Bank accession number AF275482.1). This is the first report of B. basicola in sweet potato in Argentina, a potential threat to storage root yields. |
format |
info:ar-repo/semantics/artículo |
topic_facet |
Ipomoea Batatas Thielaviopsis Basicola Argentina Podredumbre de la Raíz Batata Enfermedades de las Plantas Root Rots Fungal Diseases Sweet Potatoes Plant Diseases Enfermedades Fungosas Chalara Elegans Enfermedad Fúngica en Batata Sweet Potato Fungal Disease |
author |
Martino, Julia Andrea Di Feo, Liliana Del Valle Paccioretti, Mauro Andres Contardi, Clara Adriana Sanchez, Miguel Alejandro Ortega, Leandro Ismael Pastor, Silvina Estela |
author_facet |
Martino, Julia Andrea Di Feo, Liliana Del Valle Paccioretti, Mauro Andres Contardi, Clara Adriana Sanchez, Miguel Alejandro Ortega, Leandro Ismael Pastor, Silvina Estela |
author_sort |
Martino, Julia Andrea |
title |
First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina |
title_short |
First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina |
title_full |
First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina |
title_fullStr |
First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina |
title_full_unstemmed |
First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina |
title_sort |
first report of berkeleyomyces basicola (synonymous: thielaviopsis basicola) on roots of sweet potato (ipomoea batatas (l.) lam) in argentina |
publisher |
Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo |
publishDate |
2021-07-07 |
url |
http://hdl.handle.net/20.500.12123/10179 https://revistas.uncu.edu.ar/ojs/index.php/RFCA/article/view/4898 https://doi.org/10.48162/rev.39.027 |
work_keys_str_mv |
AT martinojuliaandrea firstreportofberkeleyomycesbasicolasynonymousthielaviopsisbasicolaonrootsofsweetpotatoipomoeabatatasllaminargentina AT difeolilianadelvalle firstreportofberkeleyomycesbasicolasynonymousthielaviopsisbasicolaonrootsofsweetpotatoipomoeabatatasllaminargentina AT pacciorettimauroandres firstreportofberkeleyomycesbasicolasynonymousthielaviopsisbasicolaonrootsofsweetpotatoipomoeabatatasllaminargentina AT contardiclaraadriana firstreportofberkeleyomycesbasicolasynonymousthielaviopsisbasicolaonrootsofsweetpotatoipomoeabatatasllaminargentina AT sanchezmiguelalejandro firstreportofberkeleyomycesbasicolasynonymousthielaviopsisbasicolaonrootsofsweetpotatoipomoeabatatasllaminargentina AT ortegaleandroismael firstreportofberkeleyomycesbasicolasynonymousthielaviopsisbasicolaonrootsofsweetpotatoipomoeabatatasllaminargentina AT pastorsilvinaestela firstreportofberkeleyomycesbasicolasynonymousthielaviopsisbasicolaonrootsofsweetpotatoipomoeabatatasllaminargentina AT martinojuliaandrea primerreportedeberkeleyomycesbasicolasinonimothielaviopsisbasicolaenraicesdebatataipomoeabatatasllamenargentina AT difeolilianadelvalle primerreportedeberkeleyomycesbasicolasinonimothielaviopsisbasicolaenraicesdebatataipomoeabatatasllamenargentina AT pacciorettimauroandres primerreportedeberkeleyomycesbasicolasinonimothielaviopsisbasicolaenraicesdebatataipomoeabatatasllamenargentina AT contardiclaraadriana primerreportedeberkeleyomycesbasicolasinonimothielaviopsisbasicolaenraicesdebatataipomoeabatatasllamenargentina AT sanchezmiguelalejandro primerreportedeberkeleyomycesbasicolasinonimothielaviopsisbasicolaenraicesdebatataipomoeabatatasllamenargentina AT ortegaleandroismael primerreportedeberkeleyomycesbasicolasinonimothielaviopsisbasicolaenraicesdebatataipomoeabatatasllamenargentina AT pastorsilvinaestela primerreportedeberkeleyomycesbasicolasinonimothielaviopsisbasicolaenraicesdebatataipomoeabatatasllamenargentina |
_version_ |
1756008141515915264 |
spelling |
oai:localhost:20.500.12123-101792021-09-03T17:25:57Z First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina Primer reporte de Berkeleyomyces basicola (sinónimo: Thielaviopsis basicola) en raíces de batata (Ipomoea batatas (L.) Lam) en Argentina Martino, Julia Andrea Di Feo, Liliana Del Valle Paccioretti, Mauro Andres Contardi, Clara Adriana Sanchez, Miguel Alejandro Ortega, Leandro Ismael Pastor, Silvina Estela Ipomoea Batatas Thielaviopsis Basicola Argentina Podredumbre de la Raíz Batata Enfermedades de las Plantas Root Rots Fungal Diseases Sweet Potatoes Plant Diseases Enfermedades Fungosas Chalara Elegans Enfermedad Fúngica en Batata Sweet Potato Fungal Disease Symptomatic sweet potato cv Arapey INIA samples were collected from a commercial production field in Colonia Molina, Guaymallén department, Mendoza province, Argentina. They showed dark rounded lesions, sometimes coalescing with white granular mycelium. Fungus was obtained from symptomatic sweet potatoes, which represented the generalized infection that affected the crop. They were seeded in PDA with streptomycin sulfate and incubated for seven days at 21°C, alternating white/black (UV400nm) light. Observations with an optical microscope revealed the presence of hyaline, not septated, cylindrical endoconidia with rounded ends. They were 8-16 μm length and 4–6 μm width. Phialides were 43-46 μm length, rounded bases (7-9 μm width) and tapering to the neck´s tip (4-6 μm width). Brown chlamydospores (aleuriospores), 9-13 μm length and 8-12 μm width, in chains of 2-8 spores were observed. For molecular identification, total genomic DNA was extracted. ITS fragment of 565 pb was amplified using ITS5/ITS4 primers and sequenced. The sequence indicated 99% identity with Berkeleyomyces basicola (synonymous: Thielaviopsis basicola). This was deposited in GenBank as (KX580957) (CBS: C430.74, Gen Bank accession number AF275482.1). This is the first report of B. basicola in sweet potato in Argentina, a potential threat to storage root yields. Muestras sintomáticas de batata cv Arapey INIA, fueron recolectadas en lotes de producción comercial en Colonia Molina, Guaymallén, Mendoza, Argentina. Estas presentaban lesiones redondeadas, oscuras, frecuentemente coalescentes, con micelio granuloso blanco. El hongo fue aislado a partir de batatas sintomáticas que representaban la infección generalizada del cultivo. Los aislamientos fueron sembrados en APG con sulfato de estreptomicina e incubados durante siete días a 21°C con alternancia de luz blanca/luz negra (UV-400 nm). Las observaciones al microscopio óptico revelaron la presencia de endoconidios hialinos, no-septados, cilíndricos con extremos redondeados (8-16 x 4-6 µm), fiálides (43-46 µm de largo) de base redondeada (7-9 µm) y cuello que se estrecha hacia la punta (4-6 µm). También se observaron clamidosporas marrones (aleuriesporas) de 9-13 x 8-12 µm, en cadenas con 1-7 septos. El ADN genómico fue extraído para la identificación molecular. Un fragmento ITS de 565 pb fue amplificado usando los iniciadores ITS5/ITS4 y secuenciado. La secuencia reveló 99 % de identidad con Berkeleyomyces basicola (sinónimo: Thielaviopsis basicola) y está depositada en el GenBank como KX580957 (CBS: C430.74, GenBank accession number AF275482.1). Esta constituye el primer reporte de B. basicola en el cultivo de batata en Argentina y una amenaza potencial para la producción de raíces reservantes. Instituto de Patología Vegetal Fil: Martino, Julia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Di Feo, Liliana Del Valle. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Paccioretti, Mauro Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Contardi, Clara Adriana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Agencia De Extensión Rural Luján de Cuyo; Argentina Fil: Sanchez, Miguel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Sanchez, Miguel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Ortega, Leandro Ismael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2021-09-03T17:05:07Z 2021-09-03T17:05:07Z 2021-07-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10179 https://revistas.uncu.edu.ar/ojs/index.php/RFCA/article/view/4898 0370-4661 (print) 1853-8665 (online) https://doi.org/10.48162/rev.39.027 eng info:eu-repo/semantics/openAccess application/pdf Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo Revista de la Facultad de Ciencias Agrarias / UNCuyo 53 (1) : 283-287 (Enero-junio 2021) |