New Bruce-ladder multiplex PCR assay for the biovar typing of Brucella suis and the discrimination of Brucella suis and Brucella canis
Rapid and specific identification of Brucella suis at the biovar level is necessary because some of the biovars that infect animals are pathogenic for humans. None of the molecular typing methods described so far are able to discriminate B. suis biovars in a single test and differentiation of B. suis from Brucella canis by molecular approaches can be difficult. This article describes a new multiplex PCR assay, Suis-ladder, for fast and accurate identification of B. suis at the biovar level and the differentiation of B. suis, B. canis and Brucella microti. An advancement of the original Bruce-ladder PCR protocol which allows the correct discrimination of all known Brucella species is also described.
Main Authors: | , |
---|---|
Format: | http://purl.org/coar/resource_type/c_6501 biblioteca |
Language: | eng |
Published: |
Revista de Microbiología Veterinaria
2011-06-28
|
Subjects: | Brucella suis, Brucella canis, Bruce-ladder, PCR, |
Online Access: | http://hdl.handle.net/11056/22276 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:https:--repositorio.una.ac.cr:11056-22276 |
---|---|
record_format |
koha |
spelling |
oai:https:--repositorio.una.ac.cr:11056-222762021-12-03T22:59:37Z New Bruce-ladder multiplex PCR assay for the biovar typing of Brucella suis and the discrimination of Brucella suis and Brucella canis López-Goñi, Ignacio García-Yoldi, David Brucella suis, Brucella canis, Bruce-ladder, PCR Rapid and specific identification of Brucella suis at the biovar level is necessary because some of the biovars that infect animals are pathogenic for humans. None of the molecular typing methods described so far are able to discriminate B. suis biovars in a single test and differentiation of B. suis from Brucella canis by molecular approaches can be difficult. This article describes a new multiplex PCR assay, Suis-ladder, for fast and accurate identification of B. suis at the biovar level and the differentiation of B. suis, B. canis and Brucella microti. An advancement of the original Bruce-ladder PCR protocol which allows the correct discrimination of all known Brucella species is also described. Universidad Nacional Escuela de Medicina Veterinaria 2021-12-03T22:59:36Z 2021-12-03T22:59:36Z 2011-06-28 http://purl.org/coar/resource_type/c_6501 03781135 http://hdl.handle.net/11056/22276 10.1016/j.vetmic.2011.06.035 eng Acceso abierto application/pdf Revista de Microbiología Veterinaria |
institution |
UNA CR |
collection |
DSpace |
country |
Costa Rica |
countrycode |
CR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-una-cr |
tag |
biblioteca |
region |
America Central |
libraryname |
Biblioteca de la Facultad de Ciencias de la Tierra y el Mar de la UNA CR |
language |
eng |
topic |
Brucella suis, Brucella canis, Bruce-ladder, PCR Brucella suis, Brucella canis, Bruce-ladder, PCR |
spellingShingle |
Brucella suis, Brucella canis, Bruce-ladder, PCR Brucella suis, Brucella canis, Bruce-ladder, PCR López-Goñi, Ignacio García-Yoldi, David New Bruce-ladder multiplex PCR assay for the biovar typing of Brucella suis and the discrimination of Brucella suis and Brucella canis |
description |
Rapid and specific identification of Brucella suis at the biovar level is necessary because
some of the biovars that infect animals are pathogenic for humans. None of the molecular
typing methods described so far are able to discriminate B. suis biovars in a single test and
differentiation of B. suis from Brucella canis by molecular approaches can be difficult. This
article describes a new multiplex PCR assay, Suis-ladder, for fast and accurate
identification of B. suis at the biovar level and the differentiation of B. suis, B. canis and
Brucella microti. An advancement of the original Bruce-ladder PCR protocol which allows
the correct discrimination of all known Brucella species is also described. |
format |
http://purl.org/coar/resource_type/c_6501 |
topic_facet |
Brucella suis, Brucella canis, Bruce-ladder, PCR |
author |
López-Goñi, Ignacio García-Yoldi, David |
author_facet |
López-Goñi, Ignacio García-Yoldi, David |
author_sort |
López-Goñi, Ignacio |
title |
New Bruce-ladder multiplex PCR assay for the biovar typing of Brucella suis and the discrimination of Brucella suis and Brucella canis |
title_short |
New Bruce-ladder multiplex PCR assay for the biovar typing of Brucella suis and the discrimination of Brucella suis and Brucella canis |
title_full |
New Bruce-ladder multiplex PCR assay for the biovar typing of Brucella suis and the discrimination of Brucella suis and Brucella canis |
title_fullStr |
New Bruce-ladder multiplex PCR assay for the biovar typing of Brucella suis and the discrimination of Brucella suis and Brucella canis |
title_full_unstemmed |
New Bruce-ladder multiplex PCR assay for the biovar typing of Brucella suis and the discrimination of Brucella suis and Brucella canis |
title_sort |
new bruce-ladder multiplex pcr assay for the biovar typing of brucella suis and the discrimination of brucella suis and brucella canis |
publisher |
Revista de Microbiología Veterinaria |
publishDate |
2011-06-28 |
url |
http://hdl.handle.net/11056/22276 |
work_keys_str_mv |
AT lopezgoniignacio newbruceladdermultiplexpcrassayforthebiovartypingofbrucellasuisandthediscriminationofbrucellasuisandbrucellacanis AT garciayoldidavid newbruceladdermultiplexpcrassayforthebiovartypingofbrucellasuisandthediscriminationofbrucellasuisandbrucellacanis |
_version_ |
1756475265711603712 |