Programmed cell death activated by Rose Bengal in Arabidopsis thaliana cell suspension cultures requires functional chloroplasts.
15 páginas. -- The definitive version is available at http://jxb.oxfordjournals.org
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| Format: | artículo biblioteca |
| Language: | English |
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Oxford University Press
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| Subjects: | Arabidopsis cell cultures, Rose Bengal, Photosensitizers, Programmed cell death, Reactive oxygen species, Singlet oxygen, Transcriptional defence responses, |
| Online Access: | http://hdl.handle.net/10261/98558 |
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dig-irnasa-es-10261-985582021-12-28T15:54:51Z Programmed cell death activated by Rose Bengal in Arabidopsis thaliana cell suspension cultures requires functional chloroplasts. Gutiérrez Merino, Jorge González Pérez, Sergio García-García, Francisco Daly, Cara T. Lorenzo, Óscar Revuelta Doval, José Luis McCabe, Paul F. Arellano, Juan B. Arabidopsis cell cultures Rose Bengal Photosensitizers Programmed cell death Reactive oxygen species Singlet oxygen Transcriptional defence responses 15 páginas. -- The definitive version is available at http://jxb.oxfordjournals.org Light-grown Arabidopsis thaliana cell suspension culture (ACSC) were subjected to mild photooxidative damage with Rose Bengal (RB) with the aim of gaining a better understanding of singlet oxygen-mediated defence responses in plants. Additionally, ACSC were treated with H2O2 at concentrations that induced comparable levels of protein oxidation damage. Under low to medium light conditions, both RB and H2O2 treatments activated transcriptional defence responses and inhibited photosynthetic activity, but they differed in that programmed cell death (PCD) was only observed in cells treated with RB. When dark-grown ACSC were subjected to RB in the light, PCD was suppressed, indicating that the singlet oxygen-mediated signalling pathway in ACSC requires functional chloroplasts. Analysis of up-regulated transcripts in light-grown ACSC, treated with RB in the light, showed that both singlet oxygen-responsive transcripts and transcripts with a key role in hormone-activated PCD (i.e. ethylene and jasmonic acid) were present. A co-regulation analysis proved that ACSC treated with RB exhibited higher correlation with the conditional fluorescence (flu) mutant than with other singlet oxygen-producing mutants or wild-type plants subjected to high light. However, there was no evidence for the up-regulation of EDS1, suggesting that activation of PCD was not associated with the EXECUTER- and EDS1-dependent signalling pathway described in the flu mutant. Indigo Carmine and Methylene Violet, two photosensitizers unable to enter chloroplasts, did not activate transcriptional defence responses in ACSC; however, whether this was due to their location or to their inherently low singlet oxygen quantum efficiencies was not determined. This work is funded by the Junta de Castilla y León (ref: CSI002A10-2). CTD was funded by the Irish Research Council for Science, Engineering and Technology. Peer reviewed 2014-07 artículo http://purl.org/coar/resource_type/c_6501 Journal of Experimental Botany (2014).65(12):3081-95. http://hdl.handle.net/10261/98558 10.1093/jxb/eru151 24723397 en Publisher's version http://dx.doi.org/10.1093/jxb/eru151 No open Oxford University Press |
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Arabidopsis cell cultures Rose Bengal Photosensitizers Programmed cell death Reactive oxygen species Singlet oxygen Transcriptional defence responses Arabidopsis cell cultures Rose Bengal Photosensitizers Programmed cell death Reactive oxygen species Singlet oxygen Transcriptional defence responses |
| spellingShingle |
Arabidopsis cell cultures Rose Bengal Photosensitizers Programmed cell death Reactive oxygen species Singlet oxygen Transcriptional defence responses Arabidopsis cell cultures Rose Bengal Photosensitizers Programmed cell death Reactive oxygen species Singlet oxygen Transcriptional defence responses Gutiérrez Merino, Jorge González Pérez, Sergio García-García, Francisco Daly, Cara T. Lorenzo, Óscar Revuelta Doval, José Luis McCabe, Paul F. Arellano, Juan B. Programmed cell death activated by Rose Bengal in Arabidopsis thaliana cell suspension cultures requires functional chloroplasts. |
| description |
15 páginas. -- The definitive version is available at http://jxb.oxfordjournals.org |
| format |
artículo |
| topic_facet |
Arabidopsis cell cultures Rose Bengal Photosensitizers Programmed cell death Reactive oxygen species Singlet oxygen Transcriptional defence responses |
| author |
Gutiérrez Merino, Jorge González Pérez, Sergio García-García, Francisco Daly, Cara T. Lorenzo, Óscar Revuelta Doval, José Luis McCabe, Paul F. Arellano, Juan B. |
| author_facet |
Gutiérrez Merino, Jorge González Pérez, Sergio García-García, Francisco Daly, Cara T. Lorenzo, Óscar Revuelta Doval, José Luis McCabe, Paul F. Arellano, Juan B. |
| author_sort |
Gutiérrez Merino, Jorge |
| title |
Programmed cell death activated by Rose Bengal in Arabidopsis thaliana cell suspension cultures requires functional chloroplasts. |
| title_short |
Programmed cell death activated by Rose Bengal in Arabidopsis thaliana cell suspension cultures requires functional chloroplasts. |
| title_full |
Programmed cell death activated by Rose Bengal in Arabidopsis thaliana cell suspension cultures requires functional chloroplasts. |
| title_fullStr |
Programmed cell death activated by Rose Bengal in Arabidopsis thaliana cell suspension cultures requires functional chloroplasts. |
| title_full_unstemmed |
Programmed cell death activated by Rose Bengal in Arabidopsis thaliana cell suspension cultures requires functional chloroplasts. |
| title_sort |
programmed cell death activated by rose bengal in arabidopsis thaliana cell suspension cultures requires functional chloroplasts. |
| publisher |
Oxford University Press |
| url |
http://hdl.handle.net/10261/98558 |
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