Discordant patterns of geographic variation between mitochondrial and microsatellite markers in the Mexican black iguana (Ctenosaura pectinata) in a contact zone
Aim To delimit the distribution of matrilines of the black iguana (Ctenosaura pectinata), detect potential contact zones between them, and test the hypothesis of geographic concordance in the structuring of matrilines with respect to genetic structuring across the nuclear genome. Location Pacific coast of Mexico in the states of Nayarit, Jalisco, Colima, Michoacán in the lowlands adjacent to the western end of the Mexican Volcanic Belt. Methods Tissue samples were obtained every 10-20km along a transect across the range of three previously described mitochondrial DNA (mtDNA) clades of C. pectinata. Iguanas were genotyped with 11 nuclear microsatellites and maternal lineages were inferred based on mtDNA sequences. Geographic structure and geographic concordance between biparentally and maternally inherited markers were analysed with clustering methods, FST and NST indexes as well as haplotype networks. Results Geographic structure was evident for both markers and the existence of contact zones confirmed. Nevertheless, the distribution of nuclear and mtDNA genetic variation is not geographically concordant. Four matrilines exist in the area, whereas only two nuclear clusters occur. A contact zone between nuclear clusters extends along a distance of c.60km, and introgression is evident in individuals from several localities. Main conclusions High levels of genetic diversity were detected on the western coast of Mexico. Historical and contemporaneous processes seem to shape the distribution of genetic variation in C. pectinata. There are no evident geographic, environmental or ecological barriers that coincide with genetic subdivisions, and the observed mtDNA structure is likely to be the result of past climatic changes. The less structured distribution of nuclear genetic variation is consistent with the homogenizing effect of male-biased dispersal. Our findings have implications for the taxonomy and conservation strategies for this threatened species, and highlight the fact that geographic analyses of both cytoplasmic and nuclear genetic variation are important for the meaningful inference of evolutionary and demographic histories.
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Blackwell Publishing
2011-07
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Subjects: | Contact zones, Ctenosaura pectinata, Cytonuclear discordance, Hybridization, Introgression, Mexico, Mexican Volcanic Belt, Reptiles, Squamata, |
Online Access: | http://hdl.handle.net/10261/199729 http://dx.doi.org/10.13039/501100003141 |
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dig-ipna-es-10261-1997292020-12-09T16:20:16Z Discordant patterns of geographic variation between mitochondrial and microsatellite markers in the Mexican black iguana (Ctenosaura pectinata) in a contact zone Zarza, Eugenia Reynoso, Victor H. Emerson, Brent C. Consejo Nacional de Ciencia y Tecnología (México) Contact zones Ctenosaura pectinata Cytonuclear discordance Hybridization Introgression Mexico Mexican Volcanic Belt Reptiles Squamata Aim To delimit the distribution of matrilines of the black iguana (Ctenosaura pectinata), detect potential contact zones between them, and test the hypothesis of geographic concordance in the structuring of matrilines with respect to genetic structuring across the nuclear genome. Location Pacific coast of Mexico in the states of Nayarit, Jalisco, Colima, Michoacán in the lowlands adjacent to the western end of the Mexican Volcanic Belt. Methods Tissue samples were obtained every 10-20km along a transect across the range of three previously described mitochondrial DNA (mtDNA) clades of C. pectinata. Iguanas were genotyped with 11 nuclear microsatellites and maternal lineages were inferred based on mtDNA sequences. Geographic structure and geographic concordance between biparentally and maternally inherited markers were analysed with clustering methods, FST and NST indexes as well as haplotype networks. Results Geographic structure was evident for both markers and the existence of contact zones confirmed. Nevertheless, the distribution of nuclear and mtDNA genetic variation is not geographically concordant. Four matrilines exist in the area, whereas only two nuclear clusters occur. A contact zone between nuclear clusters extends along a distance of c.60km, and introgression is evident in individuals from several localities. Main conclusions High levels of genetic diversity were detected on the western coast of Mexico. Historical and contemporaneous processes seem to shape the distribution of genetic variation in C. pectinata. There are no evident geographic, environmental or ecological barriers that coincide with genetic subdivisions, and the observed mtDNA structure is likely to be the result of past climatic changes. The less structured distribution of nuclear genetic variation is consistent with the homogenizing effect of male-biased dispersal. Our findings have implications for the taxonomy and conservation strategies for this threatened species, and highlight the fact that geographic analyses of both cytoplasmic and nuclear genetic variation are important for the meaningful inference of evolutionary and demographic histories. This work was funded by CONACyT through a PhD grant awarded to E. Zarza. Samples were collected under the permits SGPA/DGVS/03563/06. 2020-02-05T10:05:25Z 2020-02-05T10:05:25Z 2011-07 2020-02-05T10:05:26Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1111/j.1365-2699.2011.02485.x issn: 0305-0270 e-issn: 1365-2699 Journal of Biogeography 38(7): 1394-1405 (2011) http://hdl.handle.net/10261/199729 10.1111/j.1365-2699.2011.02485.x http://dx.doi.org/10.13039/501100003141 Postprint http://dx.doi.org/10.1111/j.1365-2699.2011.02485.x Sí none Blackwell Publishing |
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Contact zones Ctenosaura pectinata Cytonuclear discordance Hybridization Introgression Mexico Mexican Volcanic Belt Reptiles Squamata Contact zones Ctenosaura pectinata Cytonuclear discordance Hybridization Introgression Mexico Mexican Volcanic Belt Reptiles Squamata |
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Contact zones Ctenosaura pectinata Cytonuclear discordance Hybridization Introgression Mexico Mexican Volcanic Belt Reptiles Squamata Contact zones Ctenosaura pectinata Cytonuclear discordance Hybridization Introgression Mexico Mexican Volcanic Belt Reptiles Squamata Zarza, Eugenia Reynoso, Victor H. Emerson, Brent C. Discordant patterns of geographic variation between mitochondrial and microsatellite markers in the Mexican black iguana (Ctenosaura pectinata) in a contact zone |
description |
Aim To delimit the distribution of matrilines of the black iguana (Ctenosaura pectinata), detect potential contact zones between them, and test the hypothesis of geographic concordance in the structuring of matrilines with respect to genetic structuring across the nuclear genome. Location Pacific coast of Mexico in the states of Nayarit, Jalisco, Colima, Michoacán in the lowlands adjacent to the western end of the Mexican Volcanic Belt. Methods Tissue samples were obtained every 10-20km along a transect across the range of three previously described mitochondrial DNA (mtDNA) clades of C. pectinata. Iguanas were genotyped with 11 nuclear microsatellites and maternal lineages were inferred based on mtDNA sequences. Geographic structure and geographic concordance between biparentally and maternally inherited markers were analysed with clustering methods, FST and NST indexes as well as haplotype networks. Results Geographic structure was evident for both markers and the existence of contact zones confirmed. Nevertheless, the distribution of nuclear and mtDNA genetic variation is not geographically concordant. Four matrilines exist in the area, whereas only two nuclear clusters occur. A contact zone between nuclear clusters extends along a distance of c.60km, and introgression is evident in individuals from several localities. Main conclusions High levels of genetic diversity were detected on the western coast of Mexico. Historical and contemporaneous processes seem to shape the distribution of genetic variation in C. pectinata. There are no evident geographic, environmental or ecological barriers that coincide with genetic subdivisions, and the observed mtDNA structure is likely to be the result of past climatic changes. The less structured distribution of nuclear genetic variation is consistent with the homogenizing effect of male-biased dispersal. Our findings have implications for the taxonomy and conservation strategies for this threatened species, and highlight the fact that geographic analyses of both cytoplasmic and nuclear genetic variation are important for the meaningful inference of evolutionary and demographic histories. |
author2 |
Consejo Nacional de Ciencia y Tecnología (México) |
author_facet |
Consejo Nacional de Ciencia y Tecnología (México) Zarza, Eugenia Reynoso, Victor H. Emerson, Brent C. |
format |
artículo |
topic_facet |
Contact zones Ctenosaura pectinata Cytonuclear discordance Hybridization Introgression Mexico Mexican Volcanic Belt Reptiles Squamata |
author |
Zarza, Eugenia Reynoso, Victor H. Emerson, Brent C. |
author_sort |
Zarza, Eugenia |
title |
Discordant patterns of geographic variation between mitochondrial and microsatellite markers in the Mexican black iguana (Ctenosaura pectinata) in a contact zone |
title_short |
Discordant patterns of geographic variation between mitochondrial and microsatellite markers in the Mexican black iguana (Ctenosaura pectinata) in a contact zone |
title_full |
Discordant patterns of geographic variation between mitochondrial and microsatellite markers in the Mexican black iguana (Ctenosaura pectinata) in a contact zone |
title_fullStr |
Discordant patterns of geographic variation between mitochondrial and microsatellite markers in the Mexican black iguana (Ctenosaura pectinata) in a contact zone |
title_full_unstemmed |
Discordant patterns of geographic variation between mitochondrial and microsatellite markers in the Mexican black iguana (Ctenosaura pectinata) in a contact zone |
title_sort |
discordant patterns of geographic variation between mitochondrial and microsatellite markers in the mexican black iguana (ctenosaura pectinata) in a contact zone |
publisher |
Blackwell Publishing |
publishDate |
2011-07 |
url |
http://hdl.handle.net/10261/199729 http://dx.doi.org/10.13039/501100003141 |
work_keys_str_mv |
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