A combined field survey and molecular identification protocol for comparing forest arthropod biodiversity across spatial scales
Obtaining fundamental biodiversity metrics such as alpha, beta and gamma diversity for arthropods is often complicated by a lack of prior taxonomic information and/or taxonomic expertise, which can result in unreliable morphologically based estimates. We provide a set of standardized ecological and molecular sampling protocols that can be employed by researchers whose taxonomic skills may be limited, and where there may be a lack of robust a priori information regarding the regional pool of species. These protocols combine mass sampling of arthropods, classification of samples into parataxonomic units (PUs) and selective sampling of individuals for mtDNA sequencing to infer biological species. We sampled ten lowland rainforest plots located on the volcanic oceanic island of Réunion (Mascarene archipelago) for spiders, a group with limited taxonomic and distributional data for this region. We classified adults and juveniles into PUs and then demonstrated the reconciliation of these units with presumed biological species using mtDNA sequence data, ecological data and distributional data. Because our species assignment protocol is not reliant upon prior taxonomic information, or taxonomic expertise, it minimizes the problem of the Linnean shortfall to yield diversity estimates that can be directly compared across independent studies. Field sampling can be extended to other arthropod groups and habitats by adapting our field sampling protocol accordingly.
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John Wiley & Sons
2017-07
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Subjects: | Taxonomy, Parataxonomy, Spider, Biological species, Beta diversity, |
Online Access: | http://hdl.handle.net/10261/184915 http://dx.doi.org/10.13039/501100007757 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100004336 |
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dig-ipna-es-10261-1849152019-11-14T10:29:45Z A combined field survey and molecular identification protocol for comparing forest arthropod biodiversity across spatial scales Emerson, Brent C. Casquet, Juliane López, Heriberto Cardoso, Pedro Borges, Paulo A.V. Oromí, Pedro Strasberg, Dominique Thébaud, Christophe Agencia Canaria de Investigación, Innovación y Sociedad de la Información European Commission Université de La Réunion Ministerio de Economía y Competitividad (España) Ministerio de Agricultura, Alimentación y Medio Ambiente (España) Organismo Autónomo Parques Nacionales (España) Taxonomy Parataxonomy Spider Biological species Beta diversity Obtaining fundamental biodiversity metrics such as alpha, beta and gamma diversity for arthropods is often complicated by a lack of prior taxonomic information and/or taxonomic expertise, which can result in unreliable morphologically based estimates. We provide a set of standardized ecological and molecular sampling protocols that can be employed by researchers whose taxonomic skills may be limited, and where there may be a lack of robust a priori information regarding the regional pool of species. These protocols combine mass sampling of arthropods, classification of samples into parataxonomic units (PUs) and selective sampling of individuals for mtDNA sequencing to infer biological species. We sampled ten lowland rainforest plots located on the volcanic oceanic island of Réunion (Mascarene archipelago) for spiders, a group with limited taxonomic and distributional data for this region. We classified adults and juveniles into PUs and then demonstrated the reconciliation of these units with presumed biological species using mtDNA sequence data, ecological data and distributional data. Because our species assignment protocol is not reliant upon prior taxonomic information, or taxonomic expertise, it minimizes the problem of the Linnean shortfall to yield diversity estimates that can be directly compared across independent studies. Field sampling can be extended to other arthropod groups and habitats by adapting our field sampling protocol accordingly. This research was supported by the ERA‐Net Net‐Biome research framework, financed through Canary Island Government ACIISI grants SE‐12/02 (PO) and SE‐12/04 (BE), cofinanced by FEDER; Portuguese FCT‐NETBIOME grant 0003/2011 (PB); French ANR‐NETBIOME grant n°11‐EBIM‐001‐01 (CT); Région Réunion council for research activities (DS); Université de La Réunion contract DGADD/PE/20120585 (DS). BCE was also supported by Spanish MINECO grant CGL2013‐42589‐P, cofinanced by FEDER, and Spanish MAGRAMA grant S20141203_002597 from the Organismo Autónomo Parques Nacionales. 2019-06-26T12:40:41Z 2019-06-26T12:40:41Z 2017-07 2019-06-26T12:40:41Z artículo http://purl.org/coar/resource_type/c_6501 Molecular Ecology Resources 17(4): 694-707 (2017) 1755-098X http://hdl.handle.net/10261/184915 10.1111/1755-0998.12617 1755-0998 http://dx.doi.org/10.13039/501100007757 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100004336 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CGL2013‐42589‐P Preprint https://doi.org/10.1111/1755-0998.12617 open John Wiley & Sons |
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Taxonomy Parataxonomy Spider Biological species Beta diversity Taxonomy Parataxonomy Spider Biological species Beta diversity |
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Taxonomy Parataxonomy Spider Biological species Beta diversity Taxonomy Parataxonomy Spider Biological species Beta diversity Emerson, Brent C. Casquet, Juliane López, Heriberto Cardoso, Pedro Borges, Paulo A.V. Oromí, Pedro Strasberg, Dominique Thébaud, Christophe A combined field survey and molecular identification protocol for comparing forest arthropod biodiversity across spatial scales |
description |
Obtaining fundamental biodiversity metrics such as alpha, beta and gamma diversity for arthropods is often complicated by a lack of prior taxonomic information and/or taxonomic expertise, which can result in unreliable morphologically based estimates. We provide a set of standardized ecological and molecular sampling protocols that can be employed by researchers whose taxonomic skills may be limited, and where there may be a lack of robust a priori information regarding the regional pool of species. These protocols combine mass sampling of arthropods, classification of samples into parataxonomic units (PUs) and selective sampling of individuals for mtDNA sequencing to infer biological species. We sampled ten lowland rainforest plots located on the volcanic oceanic island of Réunion (Mascarene archipelago) for spiders, a group with limited taxonomic and distributional data for this region. We classified adults and juveniles into PUs and then demonstrated the reconciliation of these units with presumed biological species using mtDNA sequence data, ecological data and distributional data. Because our species assignment protocol is not reliant upon prior taxonomic information, or taxonomic expertise, it minimizes the problem of the Linnean shortfall to yield diversity estimates that can be directly compared across independent studies. Field sampling can be extended to other arthropod groups and habitats by adapting our field sampling protocol accordingly. |
author2 |
Agencia Canaria de Investigación, Innovación y Sociedad de la Información |
author_facet |
Agencia Canaria de Investigación, Innovación y Sociedad de la Información Emerson, Brent C. Casquet, Juliane López, Heriberto Cardoso, Pedro Borges, Paulo A.V. Oromí, Pedro Strasberg, Dominique Thébaud, Christophe |
format |
artículo |
topic_facet |
Taxonomy Parataxonomy Spider Biological species Beta diversity |
author |
Emerson, Brent C. Casquet, Juliane López, Heriberto Cardoso, Pedro Borges, Paulo A.V. Oromí, Pedro Strasberg, Dominique Thébaud, Christophe |
author_sort |
Emerson, Brent C. |
title |
A combined field survey and molecular identification protocol for comparing forest arthropod biodiversity across spatial scales |
title_short |
A combined field survey and molecular identification protocol for comparing forest arthropod biodiversity across spatial scales |
title_full |
A combined field survey and molecular identification protocol for comparing forest arthropod biodiversity across spatial scales |
title_fullStr |
A combined field survey and molecular identification protocol for comparing forest arthropod biodiversity across spatial scales |
title_full_unstemmed |
A combined field survey and molecular identification protocol for comparing forest arthropod biodiversity across spatial scales |
title_sort |
combined field survey and molecular identification protocol for comparing forest arthropod biodiversity across spatial scales |
publisher |
John Wiley & Sons |
publishDate |
2017-07 |
url |
http://hdl.handle.net/10261/184915 http://dx.doi.org/10.13039/501100007757 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100004336 |
work_keys_str_mv |
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_version_ |
1777669799721041920 |