Induction of colony formation by phytohemagglutinin stimulation of rainbow trout kidney in fibrin cultures

The cells from rainbow trout kidney, its main hematopoietic organ, are able to proliferate by forming colonies in fibrin-clots in the presence of phytohemagglutinin (a mitogen of mammalian T lymphocytes). The addition of phytohemagglutinin induced colonies formed by lymphocytes, eccentric-nuclei cells, multinucleated cells and large nucleated cells. The heterogeneity of this response suggests that, in this primitive animal the immunological reactions could be simpler than in mammals, one unique mitogen giving rise to proliferation of more than one cellular type. This method could be applied to the study of the immune response in the case of viral or bacterial infections, in trouts as well as in other animals including men.

Bibliographic Details

Main Author:	Coll Morales, J.
Format:	journal article biblioteca
Language:	eng
Published:	1990
Online Access:	http://hdl.handle.net/20.500.12792/4993
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