Antimicrobial activity of pediocin-producing Lactococcus lactis on Listeria monocytogenes, Staphylococcus aureus and Escherichia coli O157H7 in cheese
The antimicrobial activity of two pediocin-producing transformants obtained from wild strains of Lactococcus lactis on the survival of Listeria monocytogenes, Staphylococcus aureus and Escherichia coli O157H7 during cheese ripening was investigated. Cheeses were manufactured from milk inoculated with the three pathogens, each at approximately 6logcfumL-1. Pediococcus acidilactici 347 (Ped+), Lc. lactis ESI 153, Lc. lactis ESI 515 (Nis+) and their respective pediocin-producing transformants Lc. lactis CL1 (Ped+) and Lc. lactis CL2 (Nis+, Ped +) were added at 1% as adjuncts to the starter culture. After 30d, L. monocytogenes, S. aureus and E. coli O157H7 counts were 5.30, 5.16 and 4.14logcfug-1 in control cheese made without adjunct culture. On day 30, pediocin-producing derivatives Lc. lactis CL1 and Lc. lactis CL2 lowered L. monocytogenes counts by 2.97 and 1.64logunits, S. aureus by 0.98 and 0.40logunits, and E. coli O157H7 by 0.84 and 1.69logunits with respect to control cheese. All cheeses made with nisin-producing LAB exhibited bacteriocin activity throughout ripening. Pediocin activity was only detected throughout the whole ripening period in cheese with Lc. lactis CL1. Because of the antimicrobial activity of pediocin PA-1, its production in situ by strains of LAB growing efficiently in milk would extend the application of this bacteriocin in cheese manufacture. © 2004 Elsevier Ltd. All rights reserved.
Main Authors: | , , , , , |
---|---|
Format: | journal article biblioteca |
Language: | eng |
Published: |
2005
|
Online Access: | http://hdl.handle.net/20.500.12792/3781 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-inia-es-20.500.12792-3781 |
---|---|
record_format |
koha |
spelling |
dig-inia-es-20.500.12792-37812020-12-15T09:52:43Z Antimicrobial activity of pediocin-producing Lactococcus lactis on Listeria monocytogenes, Staphylococcus aureus and Escherichia coli O157H7 in cheese Rodríguez, E. Calzada, J. Arqués, J. L. Rodríguez, J. M. Nuñez, M. Medina, M. The antimicrobial activity of two pediocin-producing transformants obtained from wild strains of Lactococcus lactis on the survival of Listeria monocytogenes, Staphylococcus aureus and Escherichia coli O157H7 during cheese ripening was investigated. Cheeses were manufactured from milk inoculated with the three pathogens, each at approximately 6logcfumL-1. Pediococcus acidilactici 347 (Ped+), Lc. lactis ESI 153, Lc. lactis ESI 515 (Nis+) and their respective pediocin-producing transformants Lc. lactis CL1 (Ped+) and Lc. lactis CL2 (Nis+, Ped +) were added at 1% as adjuncts to the starter culture. After 30d, L. monocytogenes, S. aureus and E. coli O157H7 counts were 5.30, 5.16 and 4.14logcfug-1 in control cheese made without adjunct culture. On day 30, pediocin-producing derivatives Lc. lactis CL1 and Lc. lactis CL2 lowered L. monocytogenes counts by 2.97 and 1.64logunits, S. aureus by 0.98 and 0.40logunits, and E. coli O157H7 by 0.84 and 1.69logunits with respect to control cheese. All cheeses made with nisin-producing LAB exhibited bacteriocin activity throughout ripening. Pediocin activity was only detected throughout the whole ripening period in cheese with Lc. lactis CL1. Because of the antimicrobial activity of pediocin PA-1, its production in situ by strains of LAB growing efficiently in milk would extend the application of this bacteriocin in cheese manufacture. © 2004 Elsevier Ltd. All rights reserved. 2020-10-22T15:26:05Z 2020-10-22T15:26:05Z 2005 journal article http://hdl.handle.net/20.500.12792/3781 10.1016/j.idairyj.2004.05.004 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access |
institution |
INIA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inia-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del INIA España |
language |
eng |
description |
The antimicrobial activity of two pediocin-producing transformants obtained from wild strains of Lactococcus lactis on the survival of Listeria monocytogenes, Staphylococcus aureus and Escherichia coli O157H7 during cheese ripening was investigated. Cheeses were manufactured from milk inoculated with the three pathogens, each at approximately 6logcfumL-1. Pediococcus acidilactici 347 (Ped+), Lc. lactis ESI 153, Lc. lactis ESI 515 (Nis+) and their respective pediocin-producing transformants Lc. lactis CL1 (Ped+) and Lc. lactis CL2 (Nis+, Ped +) were added at 1% as adjuncts to the starter culture. After 30d, L. monocytogenes, S. aureus and E. coli O157H7 counts were 5.30, 5.16 and 4.14logcfug-1 in control cheese made without adjunct culture. On day 30, pediocin-producing derivatives Lc. lactis CL1 and Lc. lactis CL2 lowered L. monocytogenes counts by 2.97 and 1.64logunits, S. aureus by 0.98 and 0.40logunits, and E. coli O157H7 by 0.84 and 1.69logunits with respect to control cheese. All cheeses made with nisin-producing LAB exhibited bacteriocin activity throughout ripening. Pediocin activity was only detected throughout the whole ripening period in cheese with Lc. lactis CL1. Because of the antimicrobial activity of pediocin PA-1, its production in situ by strains of LAB growing efficiently in milk would extend the application of this bacteriocin in cheese manufacture. © 2004 Elsevier Ltd. All rights reserved. |
format |
journal article |
author |
Rodríguez, E. Calzada, J. Arqués, J. L. Rodríguez, J. M. Nuñez, M. Medina, M. |
spellingShingle |
Rodríguez, E. Calzada, J. Arqués, J. L. Rodríguez, J. M. Nuñez, M. Medina, M. Antimicrobial activity of pediocin-producing Lactococcus lactis on Listeria monocytogenes, Staphylococcus aureus and Escherichia coli O157H7 in cheese |
author_facet |
Rodríguez, E. Calzada, J. Arqués, J. L. Rodríguez, J. M. Nuñez, M. Medina, M. |
author_sort |
Rodríguez, E. |
title |
Antimicrobial activity of pediocin-producing Lactococcus lactis on Listeria monocytogenes, Staphylococcus aureus and Escherichia coli O157H7 in cheese |
title_short |
Antimicrobial activity of pediocin-producing Lactococcus lactis on Listeria monocytogenes, Staphylococcus aureus and Escherichia coli O157H7 in cheese |
title_full |
Antimicrobial activity of pediocin-producing Lactococcus lactis on Listeria monocytogenes, Staphylococcus aureus and Escherichia coli O157H7 in cheese |
title_fullStr |
Antimicrobial activity of pediocin-producing Lactococcus lactis on Listeria monocytogenes, Staphylococcus aureus and Escherichia coli O157H7 in cheese |
title_full_unstemmed |
Antimicrobial activity of pediocin-producing Lactococcus lactis on Listeria monocytogenes, Staphylococcus aureus and Escherichia coli O157H7 in cheese |
title_sort |
antimicrobial activity of pediocin-producing lactococcus lactis on listeria monocytogenes, staphylococcus aureus and escherichia coli o157h7 in cheese |
publishDate |
2005 |
url |
http://hdl.handle.net/20.500.12792/3781 |
work_keys_str_mv |
AT rodrigueze antimicrobialactivityofpediocinproducinglactococcuslactisonlisteriamonocytogenesstaphylococcusaureusandescherichiacolio157h7incheese AT calzadaj antimicrobialactivityofpediocinproducinglactococcuslactisonlisteriamonocytogenesstaphylococcusaureusandescherichiacolio157h7incheese AT arquesjl antimicrobialactivityofpediocinproducinglactococcuslactisonlisteriamonocytogenesstaphylococcusaureusandescherichiacolio157h7incheese AT rodriguezjm antimicrobialactivityofpediocinproducinglactococcuslactisonlisteriamonocytogenesstaphylococcusaureusandescherichiacolio157h7incheese AT nunezm antimicrobialactivityofpediocinproducinglactococcuslactisonlisteriamonocytogenesstaphylococcusaureusandescherichiacolio157h7incheese AT medinam antimicrobialactivityofpediocinproducinglactococcuslactisonlisteriamonocytogenesstaphylococcusaureusandescherichiacolio157h7incheese |
_version_ |
1758004868063166464 |