Prevalence and Population Diversity of Listeria monocytogenes Isolated from Dairy Cattle Farms in the Cantabria Region of Spain

Prevalence and Population Diversity of Listeria monocytogenes Isolated from Dairy Cattle Farms in the Cantabria Region of Spain

20 Pág. Departamento de Tecnología de Alimentos​​

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Main Authors: Varsaki, Athanasia, Ortiz, Sagrario, Santorum, Patricia, López, Pilar, López-Alonso, Victoria, Hernández, Marta, Abad, David, Rodríguez-Grande, Jorge, Ocampo-Sosa, Alain A., Martínez-Suárez, Joaquín V.
Other Authors: Ministerio de Economía y Competitividad (España)
Format: artículo biblioteca
Language:English
Published: Multidisciplinary Digital Publishing Institute 2022-09-19
Subjects:Listeria monocytogenes, Antimicrobial resistance, Dairy cattle, Manure, Sequence type, Virulence, Whole-genome sequencing,
Online Access:http://hdl.handle.net/10261/295767
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100004837
https://api.elsevier.com/content/abstract/scopus_id/85138527220
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spelling dig-inia-es-10261-2957672023-02-24T02:40:23Z Prevalence and Population Diversity of Listeria monocytogenes Isolated from Dairy Cattle Farms in the Cantabria Region of Spain Varsaki, Athanasia Ortiz, Sagrario Santorum, Patricia López, Pilar López-Alonso, Victoria Hernández, Marta Abad, David Rodríguez-Grande, Jorge Ocampo-Sosa, Alain A. Martínez-Suárez, Joaquín V. Ministerio de Economía y Competitividad (España) Ministerio de Ciencia e Innovación (España) CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Varsaki, Athanasia [0000-0001-6739-8859] López-Alonso, Victoria [0000-0001-8129-8352] Hernández, Marta [0000-0001-6914-9987] Rodríguez-Grande, Jorge [0000-0002-8229-6049] Martínez-Suárez, Joaquín V. [0000-0003-4306-3223] Listeria monocytogenes Antimicrobial resistance Dairy cattle Manure Sequence type Virulence Whole-genome sequencing 20 Pág. Departamento de Tecnología de Alimentos​​ Listeria monocytogenes is an opportunistic pathogen that is widely distributed in the environment. Here we show the prevalence and transmission of L. monocytogenes in dairy farms in the Cantabria region, on the northern coast of Spain. A total of 424 samples was collected from 14 dairy farms (5 organic and 9 conventional) and 211 L. monocytogenes isolates were recovered following conventional microbiological methods. There were no statistically significant differences in antimicrobial resistance ratios between organic and conventional farms. A clonal relationship among the isolates was assessed by pulsed field gel electrophoresis (PFGE) analysis and 64 different pulsotypes were obtained. Most isolates (89%, n = 187) were classified as PCR serogroup IVb by using a multiplex PCR assay. In this case, 45 isolates of PCR serogroup IVb were whole genome-sequenced to perform a further analysis at genomic level. In silico MLST analysis showed the presence of 12 sequence types (ST), of which ST1, ST54 and ST666 were the most common. Our data indicate that the environment of cattle farms retains a high incidence of L. monocytogenes, including subtypes involved in human listeriosis reports and outbreaks. This pathogen is shed in the feces and could easily colonize dairy products, as a result of fecal contamination. Effective herd and manure management are needed in order to prevent possible outbreaks. This work was supported by Research Project grants RTA08-099, RTA2008-00080-C02, RTA2014-00045-C03-01 (INIA and FEDER) from the Spanish Ministry of Economy and Competitiveness and RTI2018-098267-R-C31 (INIA and FEDER) from the Spanish Ministry of Science and Innovation Peer reviewed 2023-02-23T12:46:41Z 2023-02-23T12:46:41Z 2022-09-19 artículo Animals 12: e2477 (2022) http://hdl.handle.net/10261/295767 10.3390/ani12182477 2076-2615 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100004837 36139336 2-s2.0-85138527220 https://api.elsevier.com/content/abstract/scopus_id/85138527220 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MICINN//RTA2008-00080-C02-01/ES/Diseño de un microarray de DNA para la detección rápida de los subtipos moleculares de Listeria monocytogenes: aplicación al estudio de la supervivencia de las diferentes cepas tras el procesado de productos cárnicos de cerdo ibérico/ info:eu-repo/grantAgreement/MINECO//RTA2014-00045-C03-01/ES/Supervivencia de Listeria monocytogenes sobre superficies de contacto con alimentos: un abordaje multidisciplinar de un problema complejo/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-098267-R-C31/ES/ALTERNATIVAS AL USO DE DESINFECTANTES EN LA INDUSTRIA ALIMENTARIA DIRIGIDAS A REDUCIR LA SUPERVIVENCIA DE LISTERIA MONOCYTOGENES Y SALMONELLA ENTERICA SOBRE LAS SUPERFICIES/ Animals : an open access journal from MDPI Publisher's version https://pubmed.ncbi.nlm.nih.gov/36139336/ Sí open Multidisciplinary Digital Publishing Institute
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic Listeria monocytogenes
Antimicrobial resistance
Dairy cattle
Manure
Sequence type
Virulence
Whole-genome sequencing
Listeria monocytogenes
Antimicrobial resistance
Dairy cattle
Manure
Sequence type
Virulence
Whole-genome sequencing
spellingShingle Listeria monocytogenes
Antimicrobial resistance
Dairy cattle
Manure
Sequence type
Virulence
Whole-genome sequencing
Listeria monocytogenes
Antimicrobial resistance
Dairy cattle
Manure
Sequence type
Virulence
Whole-genome sequencing
Varsaki, Athanasia
Ortiz, Sagrario
Santorum, Patricia
López, Pilar
López-Alonso, Victoria
Hernández, Marta
Abad, David
Rodríguez-Grande, Jorge
Ocampo-Sosa, Alain A.
Martínez-Suárez, Joaquín V.
Prevalence and Population Diversity of Listeria monocytogenes Isolated from Dairy Cattle Farms in the Cantabria Region of Spain
description 20 Pág. Departamento de Tecnología de Alimentos​​
author2 Ministerio de Economía y Competitividad (España)
author_facet Ministerio de Economía y Competitividad (España)
Varsaki, Athanasia
Ortiz, Sagrario
Santorum, Patricia
López, Pilar
López-Alonso, Victoria
Hernández, Marta
Abad, David
Rodríguez-Grande, Jorge
Ocampo-Sosa, Alain A.
Martínez-Suárez, Joaquín V.
format artículo
topic_facet Listeria monocytogenes
Antimicrobial resistance
Dairy cattle
Manure
Sequence type
Virulence
Whole-genome sequencing
author Varsaki, Athanasia
Ortiz, Sagrario
Santorum, Patricia
López, Pilar
López-Alonso, Victoria
Hernández, Marta
Abad, David
Rodríguez-Grande, Jorge
Ocampo-Sosa, Alain A.
Martínez-Suárez, Joaquín V.
author_sort Varsaki, Athanasia
title Prevalence and Population Diversity of Listeria monocytogenes Isolated from Dairy Cattle Farms in the Cantabria Region of Spain
title_short Prevalence and Population Diversity of Listeria monocytogenes Isolated from Dairy Cattle Farms in the Cantabria Region of Spain
title_full Prevalence and Population Diversity of Listeria monocytogenes Isolated from Dairy Cattle Farms in the Cantabria Region of Spain
title_fullStr Prevalence and Population Diversity of Listeria monocytogenes Isolated from Dairy Cattle Farms in the Cantabria Region of Spain
title_full_unstemmed Prevalence and Population Diversity of Listeria monocytogenes Isolated from Dairy Cattle Farms in the Cantabria Region of Spain
title_sort prevalence and population diversity of listeria monocytogenes isolated from dairy cattle farms in the cantabria region of spain
publisher Multidisciplinary Digital Publishing Institute
publishDate 2022-09-19
url http://hdl.handle.net/10261/295767
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100004837
https://api.elsevier.com/content/abstract/scopus_id/85138527220
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