Humoral and in vivo cellular immunity against the raw insect-derived recombinant Leishmania infantum antigens KMPII, TRYP, LACK, and papLe22 in dogs from an endemic area

Leishmania infantum causes visceral leishmaniasis, a severe zoonotic and systemic disease that is fatal if left untreated. Identification of the antigens involved in Leishmania-specific protective immune response is a research priority for the development of effective control measures. For this purpose, we evaluated, in 27 dogs from an enzootic zone, specific humoral and cellular immune response by delayed-type hypersensitivity (DTH) skin test both against total L. infantum antigen and the raw Trichoplusia ni insect-derived kinetoplastid membrane protein-11 (rKMPII), tryparedoxin peroxidase (rTRYP), Leishmania homologue of receptors for activated C kinase (rLACK), and 22-kDa potentially aggravating protein of Leishmania (rpapLe22) antigens from this parasite. rTRYP induced the highest number of positive DTH responses (55% of leishmanin skin test [LST]-positive dogs), showing that TRYP antigen is an important T cell immunogen, and it could be a promising vaccine candidate against this disease. When TRYP-DTH and KMPII-DTH tests were evaluated in parallel, 82% of LST-positive dogs were detected, suggesting that both antigens could be considered as components of a standardized DTH immunodiagnostic tool for dogs. Copyright © 2010 by The American Society of Tropical Medicine and Hygiene.

Detalles Bibliográficos

Autores principales:	Todolí, F., Solano-Gallego, L., de Juan, R., Morell, P., Núñez, M. d. C., Lasa, R., Gómez-Sebastián, S., Escribano, J. M., Alberola, J., Rodríguez-Cortés, A.
Formato:	journal article biblioteca
Idioma:	English
Publicado:	American Society of Tropical Medicine and Hygiene 2010
Acceso en línea:	http://hdl.handle.net/20.500.12792/4480 http://hdl.handle.net/10261/294671
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