Persistence of the bovine spongiform encephalopathy infectious agent in sewage
Horizontal transmission of prion diseases through the environment represents a considerable concern. Prions are extremely resistant to inactivation and are thought to enter the environment after burial of animal mortalities or through biosolids from wastewater treatment plants. In addition, deposition of prions in the environment through biological fluids and/or faeces has been proved in the last years. Little is known about the behaviour of prion infectivity in the environment. In this study, the persistence of BSE infectious agent in sewage has been assessed by both PrP Res immunoblotting and mouse bioassay in a long-term incubation study. Results indicated that no PrP Res was detected after 150 day of incubation and consistent with this, a statistical regression model estimated 2-logs decay in 151 day. In contrast, no reduction in infectivity was observed during this period. Similarly, BSE infectivity remained unaltered after incubation in PBS for 265 day, whereas PrP Res levels dropped progressively over the length of the study. These results indicate that in sewage and PBS, prion infectivity persists longer and with different dynamics than its commonly used marker PrP Res. Thus, mathematical models computed on the basis of PrP Res detection were unable to predict inactivation of prion infectivity. It is also reasonable to assume that conventional wastewater treatments with low retention times could have a very limited impact on prion infectivity. This data is essential for the development of accurate risk assessment analysis for BSE and other prion diseases in the environment. © 2012 Elsevier Inc.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2012
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Subjects: | Prions, BSE, PrPRes, Infectivity, Sewage, Environment, |
Online Access: | http://hdl.handle.net/20.500.12792/2591 http://hdl.handle.net/10261/293117 |
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dig-inia-es-10261-2931172023-02-20T07:35:59Z Persistence of the bovine spongiform encephalopathy infectious agent in sewage Maluquer de Motes, C. Espinosa Martín, Juan Carlos Esteban, A. Calvo, M. Girones, R. Torres, J. M. Prions BSE PrPRes Infectivity Sewage Environment Horizontal transmission of prion diseases through the environment represents a considerable concern. Prions are extremely resistant to inactivation and are thought to enter the environment after burial of animal mortalities or through biosolids from wastewater treatment plants. In addition, deposition of prions in the environment through biological fluids and/or faeces has been proved in the last years. Little is known about the behaviour of prion infectivity in the environment. In this study, the persistence of BSE infectious agent in sewage has been assessed by both PrP Res immunoblotting and mouse bioassay in a long-term incubation study. Results indicated that no PrP Res was detected after 150 day of incubation and consistent with this, a statistical regression model estimated 2-logs decay in 151 day. In contrast, no reduction in infectivity was observed during this period. Similarly, BSE infectivity remained unaltered after incubation in PBS for 265 day, whereas PrP Res levels dropped progressively over the length of the study. These results indicate that in sewage and PBS, prion infectivity persists longer and with different dynamics than its commonly used marker PrP Res. Thus, mathematical models computed on the basis of PrP Res detection were unable to predict inactivation of prion infectivity. It is also reasonable to assume that conventional wastewater treatments with low retention times could have a very limited impact on prion infectivity. This data is essential for the development of accurate risk assessment analysis for BSE and other prion diseases in the environment. © 2012 Elsevier Inc. 2023-02-20T07:35:59Z 2023-02-20T07:35:59Z 2012 artículo Environmental Research 117: 1-7 (2012) 0013-9351 http://hdl.handle.net/20.500.12792/2591 http://hdl.handle.net/10261/293117 10.1016/j.envres.2012.06.010 en none Elsevier |
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Prions BSE PrPRes Infectivity Sewage Environment Prions BSE PrPRes Infectivity Sewage Environment |
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Prions BSE PrPRes Infectivity Sewage Environment Prions BSE PrPRes Infectivity Sewage Environment Maluquer de Motes, C. Espinosa Martín, Juan Carlos Esteban, A. Calvo, M. Girones, R. Torres, J. M. Persistence of the bovine spongiform encephalopathy infectious agent in sewage |
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Horizontal transmission of prion diseases through the environment represents a considerable concern. Prions are extremely resistant to inactivation and are thought to enter the environment after burial of animal mortalities or through biosolids from wastewater treatment plants. In addition, deposition of prions in the environment through biological fluids and/or faeces has been proved in the last years. Little is known about the behaviour of prion infectivity in the environment. In this study, the persistence of BSE infectious agent in sewage has been assessed by both PrP Res immunoblotting and mouse bioassay in a long-term incubation study. Results indicated that no PrP Res was detected after 150 day of incubation and consistent with this, a statistical regression model estimated 2-logs decay in 151 day. In contrast, no reduction in infectivity was observed during this period. Similarly, BSE infectivity remained unaltered after incubation in PBS for 265 day, whereas PrP Res levels dropped progressively over the length of the study. These results indicate that in sewage and PBS, prion infectivity persists longer and with different dynamics than its commonly used marker PrP Res. Thus, mathematical models computed on the basis of PrP Res detection were unable to predict inactivation of prion infectivity. It is also reasonable to assume that conventional wastewater treatments with low retention times could have a very limited impact on prion infectivity. This data is essential for the development of accurate risk assessment analysis for BSE and other prion diseases in the environment. © 2012 Elsevier Inc. |
format |
artículo |
topic_facet |
Prions BSE PrPRes Infectivity Sewage Environment |
author |
Maluquer de Motes, C. Espinosa Martín, Juan Carlos Esteban, A. Calvo, M. Girones, R. Torres, J. M. |
author_facet |
Maluquer de Motes, C. Espinosa Martín, Juan Carlos Esteban, A. Calvo, M. Girones, R. Torres, J. M. |
author_sort |
Maluquer de Motes, C. |
title |
Persistence of the bovine spongiform encephalopathy infectious agent in sewage |
title_short |
Persistence of the bovine spongiform encephalopathy infectious agent in sewage |
title_full |
Persistence of the bovine spongiform encephalopathy infectious agent in sewage |
title_fullStr |
Persistence of the bovine spongiform encephalopathy infectious agent in sewage |
title_full_unstemmed |
Persistence of the bovine spongiform encephalopathy infectious agent in sewage |
title_sort |
persistence of the bovine spongiform encephalopathy infectious agent in sewage |
publisher |
Elsevier |
publishDate |
2012 |
url |
http://hdl.handle.net/20.500.12792/2591 http://hdl.handle.net/10261/293117 |
work_keys_str_mv |
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1767603436123062272 |