New diagnostic methods for vesicular diseases

Occurrence of vesicular diseases constitutes one of the principal animal health problems in swine. An effective distinction is required between the three viruses causing major indistinguishable vesicular pathologies; two members of the Picornavirus family (foot-and-mouth disease virus - FMDV) and virus causing swine vesicular disease (SVD), as well as vesicular stomatitis virus (VSV), a rhabdovirus that affects the Americas. These three diseases are classified by the OIE (International Office of Epizootics) in the list A, occupying the first three positions in that list. The conventional diagnosis of these viral diseases includes virus isolation using several cell lines and the identification of the agent by ELISA. In the serology usually the prescribed tests for trade, virus neutralisation (VN) and ELISA are used. Although these tests are official and in general allow to carry out a suit diagnosis, the development of new techniques, mainly based on molecular biology, enables to detect these viruses more fastly and with higher specificity and sensibility. Among these tests of new generation can be included polymerase chain reaction (PCR), nucleotide sequence and the use of recombinant proteins.

Bibliographic Details

Main Authors:	Blanco Lavilla, Esther, Sanchez Vizcaíno, J. M.
Format:	artículo biblioteca
Language:	English
Published:	Springer 2000
Online Access:	http://hdl.handle.net/20.500.12792/4904 http://hdl.handle.net/10261/292669
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