Effect of vitamin C on innate immune responses of rainbow trout (Oncorhynchus mykiss) leukocytes
Vitamin C, also known as ascorbic acid, is an essential micronutrient that influences a wide variety of physiological processes, including immunological functions. Although the positive effects of vitamin C supplementation on the immunological status of fish has been established in different species, the bases for these positive effects are still unknown. Hence, the aim of our study was to evaluate the in vitro effect of vitamin C on several innate immune functions of rainbow trout (Oncorhynchus mykiss) leukocyte populations. For this, we assessed the effects exerted on the established rainbow trout monocyte-macrophage cell line RTS11, and compared them to those observed in trout head kidney leukocytes. Our results demonstrate that vitamin C increases the production of reactive oxygen species and the percentage of phagocytic cells in both cell populations. On the other hand, vitamin C had no effect on the surface MHC II levels and only in the case of RTS11 cells increased the capacity of these cells to migrate towards the CK9 chemokine. Finally, vitamin C also increased the transcription of several pro-inflammatory and antimicrobial genes elicited by Escherichia coli, with some differences depending on the cell population studied. Our results contribute to further understand how vitamin C supplementation regulates the fish immune system. © 2017
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2017
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Subjects: | Vitamin C, Rainbow trout, RTS11 cells, Head kidney leukocytes, Phagocytosis, Respiratory burst, Antibacterial activities, |
Online Access: | http://hdl.handle.net/20.500.12792/2353 http://hdl.handle.net/10261/292181 |
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dig-inia-es-10261-2921812023-02-20T07:26:19Z Effect of vitamin C on innate immune responses of rainbow trout (Oncorhynchus mykiss) leukocytes Leal, E. Zarza, C. Tafalla Piñeiro, Carolina Vitamin C Rainbow trout RTS11 cells Head kidney leukocytes Phagocytosis Respiratory burst Antibacterial activities Vitamin C, also known as ascorbic acid, is an essential micronutrient that influences a wide variety of physiological processes, including immunological functions. Although the positive effects of vitamin C supplementation on the immunological status of fish has been established in different species, the bases for these positive effects are still unknown. Hence, the aim of our study was to evaluate the in vitro effect of vitamin C on several innate immune functions of rainbow trout (Oncorhynchus mykiss) leukocyte populations. For this, we assessed the effects exerted on the established rainbow trout monocyte-macrophage cell line RTS11, and compared them to those observed in trout head kidney leukocytes. Our results demonstrate that vitamin C increases the production of reactive oxygen species and the percentage of phagocytic cells in both cell populations. On the other hand, vitamin C had no effect on the surface MHC II levels and only in the case of RTS11 cells increased the capacity of these cells to migrate towards the CK9 chemokine. Finally, vitamin C also increased the transcription of several pro-inflammatory and antimicrobial genes elicited by Escherichia coli, with some differences depending on the cell population studied. Our results contribute to further understand how vitamin C supplementation regulates the fish immune system. © 2017 2023-02-20T07:26:19Z 2023-02-20T07:26:19Z 2017 artículo Fish and Shellfish Immunology 67: 179-188 (2017) 1050-4648 http://hdl.handle.net/20.500.12792/2353 http://hdl.handle.net/10261/292181 10.1016/j.fsi.2017.06.021 en none Elsevier |
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Vitamin C Rainbow trout RTS11 cells Head kidney leukocytes Phagocytosis Respiratory burst Antibacterial activities Vitamin C Rainbow trout RTS11 cells Head kidney leukocytes Phagocytosis Respiratory burst Antibacterial activities |
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Vitamin C Rainbow trout RTS11 cells Head kidney leukocytes Phagocytosis Respiratory burst Antibacterial activities Vitamin C Rainbow trout RTS11 cells Head kidney leukocytes Phagocytosis Respiratory burst Antibacterial activities Leal, E. Zarza, C. Tafalla Piñeiro, Carolina Effect of vitamin C on innate immune responses of rainbow trout (Oncorhynchus mykiss) leukocytes |
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Vitamin C, also known as ascorbic acid, is an essential micronutrient that influences a wide variety of physiological processes, including immunological functions. Although the positive effects of vitamin C supplementation on the immunological status of fish has been established in different species, the bases for these positive effects are still unknown. Hence, the aim of our study was to evaluate the in vitro effect of vitamin C on several innate immune functions of rainbow trout (Oncorhynchus mykiss) leukocyte populations. For this, we assessed the effects exerted on the established rainbow trout monocyte-macrophage cell line RTS11, and compared them to those observed in trout head kidney leukocytes. Our results demonstrate that vitamin C increases the production of reactive oxygen species and the percentage of phagocytic cells in both cell populations. On the other hand, vitamin C had no effect on the surface MHC II levels and only in the case of RTS11 cells increased the capacity of these cells to migrate towards the CK9 chemokine. Finally, vitamin C also increased the transcription of several pro-inflammatory and antimicrobial genes elicited by Escherichia coli, with some differences depending on the cell population studied. Our results contribute to further understand how vitamin C supplementation regulates the fish immune system. © 2017 |
format |
artículo |
topic_facet |
Vitamin C Rainbow trout RTS11 cells Head kidney leukocytes Phagocytosis Respiratory burst Antibacterial activities |
author |
Leal, E. Zarza, C. Tafalla Piñeiro, Carolina |
author_facet |
Leal, E. Zarza, C. Tafalla Piñeiro, Carolina |
author_sort |
Leal, E. |
title |
Effect of vitamin C on innate immune responses of rainbow trout (Oncorhynchus mykiss) leukocytes |
title_short |
Effect of vitamin C on innate immune responses of rainbow trout (Oncorhynchus mykiss) leukocytes |
title_full |
Effect of vitamin C on innate immune responses of rainbow trout (Oncorhynchus mykiss) leukocytes |
title_fullStr |
Effect of vitamin C on innate immune responses of rainbow trout (Oncorhynchus mykiss) leukocytes |
title_full_unstemmed |
Effect of vitamin C on innate immune responses of rainbow trout (Oncorhynchus mykiss) leukocytes |
title_sort |
effect of vitamin c on innate immune responses of rainbow trout (oncorhynchus mykiss) leukocytes |
publisher |
Elsevier |
publishDate |
2017 |
url |
http://hdl.handle.net/20.500.12792/2353 http://hdl.handle.net/10261/292181 |
work_keys_str_mv |
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_version_ |
1767603309695205376 |