Cytotoxicity of the mycotoxins deoxynivalenol and ochratoxin A on Caco-2 cell line in presence of resveratrol
Exposure to mycotoxins through dietary food intake involves a highly complex scenario where co-contamination of different mycotoxins has been frequently demonstrated. On the other hand, the effect of the interaction of mycotoxins with other generally considered beneficial food components, as the antioxidants, has been scarcely studied. The main goal of the present work was to assess the cytotoxic effects on Caco-2 cells of the mycotoxins deoxynivalenol (DON) and ochratoxin A (OTA), alone or combined, and to explore potential protective effects of resveratrol (RES), an antioxidant frequently found in wine. In parallel, reactive oxygen species (ROS) production has also been studied as a first approach to understand the underlying mechanism of cytotoxicity. Results indicate a higher toxic effect of the mycotoxins when they are co-exposed. This increase in cytotoxicity was not accompanied by an increase in ROS production. The co-exposure of OTA or DON with RES did not result in a decrease in cytotoxicity; on the contrary, it resulted in increased cytotoxicity not associated with an increase in ROS production. © 2015 Elsevier Ltd.
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Elsevier
2015
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Subjects: | Antioxidant, Mycotoxin co-exposition, In vitro cytotoxicity, Oxidative stress, ROS production, |
Online Access: | http://hdl.handle.net/20.500.12792/4958 http://hdl.handle.net/10261/290586 |
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dig-inia-es-10261-2905862023-02-17T12:27:15Z Cytotoxicity of the mycotoxins deoxynivalenol and ochratoxin A on Caco-2 cell line in presence of resveratrol Cano-Sancho, G. González-Arias, C. A. Ramos, A. J. Sanchis, V. Fernández-Cruz, M. L. Antioxidant Mycotoxin co-exposition In vitro cytotoxicity Oxidative stress ROS production Exposure to mycotoxins through dietary food intake involves a highly complex scenario where co-contamination of different mycotoxins has been frequently demonstrated. On the other hand, the effect of the interaction of mycotoxins with other generally considered beneficial food components, as the antioxidants, has been scarcely studied. The main goal of the present work was to assess the cytotoxic effects on Caco-2 cells of the mycotoxins deoxynivalenol (DON) and ochratoxin A (OTA), alone or combined, and to explore potential protective effects of resveratrol (RES), an antioxidant frequently found in wine. In parallel, reactive oxygen species (ROS) production has also been studied as a first approach to understand the underlying mechanism of cytotoxicity. Results indicate a higher toxic effect of the mycotoxins when they are co-exposed. This increase in cytotoxicity was not accompanied by an increase in ROS production. The co-exposure of OTA or DON with RES did not result in a decrease in cytotoxicity; on the contrary, it resulted in increased cytotoxicity not associated with an increase in ROS production. © 2015 Elsevier Ltd. 2023-02-17T12:27:15Z 2023-02-17T12:27:15Z 2015 artículo Toxicology in Vitro 29: 1639-1646 (2015) 0887-2333 http://hdl.handle.net/20.500.12792/4958 http://hdl.handle.net/10261/290586 10.1016/j.tiv.2015.06.020 en none Elsevier |
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Antioxidant Mycotoxin co-exposition In vitro cytotoxicity Oxidative stress ROS production Antioxidant Mycotoxin co-exposition In vitro cytotoxicity Oxidative stress ROS production |
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Antioxidant Mycotoxin co-exposition In vitro cytotoxicity Oxidative stress ROS production Antioxidant Mycotoxin co-exposition In vitro cytotoxicity Oxidative stress ROS production Cano-Sancho, G. González-Arias, C. A. Ramos, A. J. Sanchis, V. Fernández-Cruz, M. L. Cytotoxicity of the mycotoxins deoxynivalenol and ochratoxin A on Caco-2 cell line in presence of resveratrol |
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Exposure to mycotoxins through dietary food intake involves a highly complex scenario where co-contamination of different mycotoxins has been frequently demonstrated. On the other hand, the effect of the interaction of mycotoxins with other generally considered beneficial food components, as the antioxidants, has been scarcely studied. The main goal of the present work was to assess the cytotoxic effects on Caco-2 cells of the mycotoxins deoxynivalenol (DON) and ochratoxin A (OTA), alone or combined, and to explore potential protective effects of resveratrol (RES), an antioxidant frequently found in wine. In parallel, reactive oxygen species (ROS) production has also been studied as a first approach to understand the underlying mechanism of cytotoxicity. Results indicate a higher toxic effect of the mycotoxins when they are co-exposed. This increase in cytotoxicity was not accompanied by an increase in ROS production. The co-exposure of OTA or DON with RES did not result in a decrease in cytotoxicity; on the contrary, it resulted in increased cytotoxicity not associated with an increase in ROS production. © 2015 Elsevier Ltd. |
format |
artículo |
topic_facet |
Antioxidant Mycotoxin co-exposition In vitro cytotoxicity Oxidative stress ROS production |
author |
Cano-Sancho, G. González-Arias, C. A. Ramos, A. J. Sanchis, V. Fernández-Cruz, M. L. |
author_facet |
Cano-Sancho, G. González-Arias, C. A. Ramos, A. J. Sanchis, V. Fernández-Cruz, M. L. |
author_sort |
Cano-Sancho, G. |
title |
Cytotoxicity of the mycotoxins deoxynivalenol and ochratoxin A on Caco-2 cell line in presence of resveratrol |
title_short |
Cytotoxicity of the mycotoxins deoxynivalenol and ochratoxin A on Caco-2 cell line in presence of resveratrol |
title_full |
Cytotoxicity of the mycotoxins deoxynivalenol and ochratoxin A on Caco-2 cell line in presence of resveratrol |
title_fullStr |
Cytotoxicity of the mycotoxins deoxynivalenol and ochratoxin A on Caco-2 cell line in presence of resveratrol |
title_full_unstemmed |
Cytotoxicity of the mycotoxins deoxynivalenol and ochratoxin A on Caco-2 cell line in presence of resveratrol |
title_sort |
cytotoxicity of the mycotoxins deoxynivalenol and ochratoxin a on caco-2 cell line in presence of resveratrol |
publisher |
Elsevier |
publishDate |
2015 |
url |
http://hdl.handle.net/20.500.12792/4958 http://hdl.handle.net/10261/290586 |
work_keys_str_mv |
AT canosanchog cytotoxicityofthemycotoxinsdeoxynivalenolandochratoxinaoncaco2celllineinpresenceofresveratrol AT gonzalezariasca cytotoxicityofthemycotoxinsdeoxynivalenolandochratoxinaoncaco2celllineinpresenceofresveratrol AT ramosaj cytotoxicityofthemycotoxinsdeoxynivalenolandochratoxinaoncaco2celllineinpresenceofresveratrol AT sanchisv cytotoxicityofthemycotoxinsdeoxynivalenolandochratoxinaoncaco2celllineinpresenceofresveratrol AT fernandezcruzml cytotoxicityofthemycotoxinsdeoxynivalenolandochratoxinaoncaco2celllineinpresenceofresveratrol |
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1767603106769534976 |