Dysregulation of lipid metabolism in PLHC-1 and ZFL cells exposed to tributyltin an all-trans retinoic acid
There is increasing awareness that exposure to endocrine disrupters interferes with lipid homeostasis in vertebrates, including fish. Many of these compounds exert their action by binding to nuclear receptors, such as peroxisome proliferator-activated receptors and retinoid X receptor. This work investigates the use of fish liver cells (PLHC-1 and ZFL cells) for the screening of metabolic and lipid disrupters in the aquatic environment by assessing changes in the cell’s lipidome after exposure to the model compounds, tributyltin chloride and all-trans retinoic acid. Lipid extracts, analyzed by FIA-ESI (+/-) Orbitrap, evidenced the intracellular accumulation of triglycerides and diglycerides in both cell models after exposure 100 and 200 nM tributyltin chloride for 24 h. Exposure to 1 µM all-trans retinoic acid led to a significant accumulation of triglycerides in PLHC-1 cells, while few triglycerides were accumulated in ZFL cells. Retinoic acid (cyp26b1, cyp3a65, lrata) and lipid metabolism (fasn, scd, elovl6) related genes were up-regulated by tributyltin chloride and all-trans retinoic acid, while only all-trans retinoic acid down-regulated the expression of dgat1a. The two cell models show sensitivity and responses to tributyltin chloride and all-trans retinoic acid comparable to those previously reported in mammalian cells. These results support the use of fish liver cells as alternative models for the detection of contaminants that act as lipid disrupters in the aquatic environment.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2020-12-24
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Subjects: | Fish cells, Liver, Lipid metabolism, Gene expression, Triglycerides, |
Online Access: | http://hdl.handle.net/10261/226107 http://dx.doi.org/10.13039/501100004837 |
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dig-idaea-es-10261-2261072022-12-24T05:30:27Z Dysregulation of lipid metabolism in PLHC-1 and ZFL cells exposed to tributyltin an all-trans retinoic acid Marqueño, Anna Flores, Cintia Casado, Marta Porte Visa, Cinta Ministerio de Ciencia e Innovación (España) Porte Visa, Cinta [0000-0002-7766-5639] Porte, Cinta [0000-0002-3940-6409] Fish cells Liver Lipid metabolism Gene expression Triglycerides There is increasing awareness that exposure to endocrine disrupters interferes with lipid homeostasis in vertebrates, including fish. Many of these compounds exert their action by binding to nuclear receptors, such as peroxisome proliferator-activated receptors and retinoid X receptor. This work investigates the use of fish liver cells (PLHC-1 and ZFL cells) for the screening of metabolic and lipid disrupters in the aquatic environment by assessing changes in the cell’s lipidome after exposure to the model compounds, tributyltin chloride and all-trans retinoic acid. Lipid extracts, analyzed by FIA-ESI (+/-) Orbitrap, evidenced the intracellular accumulation of triglycerides and diglycerides in both cell models after exposure 100 and 200 nM tributyltin chloride for 24 h. Exposure to 1 µM all-trans retinoic acid led to a significant accumulation of triglycerides in PLHC-1 cells, while few triglycerides were accumulated in ZFL cells. Retinoic acid (cyp26b1, cyp3a65, lrata) and lipid metabolism (fasn, scd, elovl6) related genes were up-regulated by tributyltin chloride and all-trans retinoic acid, while only all-trans retinoic acid down-regulated the expression of dgat1a. The two cell models show sensitivity and responses to tributyltin chloride and all-trans retinoic acid comparable to those previously reported in mammalian cells. These results support the use of fish liver cells as alternative models for the detection of contaminants that act as lipid disrupters in the aquatic environment. Work financed by Ministerio de Ciencia e Innovación, under the projects CGL2014-52144-P and PGC2018-097513-B-I00 (MCIU/AEI/FEDER, UE). Anna Marqueño acknowledges a pre-doctoral fellowship BES-2015-074842. Peer reviewed 2021-01-07T09:23:03Z 2021-01-07T09:23:03Z 2020-12-24 artículo http://purl.org/coar/resource_type/c_6501 Aquatic Toxicology 227: 105733 (2020) http://hdl.handle.net/10261/226107 10.1016/j.aquatox.2020.105733 http://dx.doi.org/10.13039/501100004837 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-097513-B-I00 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CGL2014-52144-P Postprint https://doi.org/10.1016/j.aquatox.2020.105733 Sí open Elsevier |
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Fish cells Liver Lipid metabolism Gene expression Triglycerides Fish cells Liver Lipid metabolism Gene expression Triglycerides |
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Fish cells Liver Lipid metabolism Gene expression Triglycerides Fish cells Liver Lipid metabolism Gene expression Triglycerides Marqueño, Anna Flores, Cintia Casado, Marta Porte Visa, Cinta Dysregulation of lipid metabolism in PLHC-1 and ZFL cells exposed to tributyltin an all-trans retinoic acid |
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There is increasing awareness that exposure to endocrine disrupters interferes with lipid homeostasis in vertebrates, including fish. Many of these compounds exert their action by binding to nuclear receptors, such as peroxisome proliferator-activated receptors and retinoid X receptor. This work investigates the use of fish liver cells (PLHC-1 and ZFL cells) for the screening of metabolic and lipid disrupters in the aquatic environment by assessing changes in the cell’s lipidome after exposure to the model compounds, tributyltin chloride and all-trans retinoic acid. Lipid extracts, analyzed by FIA-ESI (+/-) Orbitrap, evidenced the intracellular accumulation of triglycerides and diglycerides in both cell models after exposure 100 and 200 nM tributyltin chloride for 24 h. Exposure to 1 µM all-trans retinoic acid led to a significant accumulation of triglycerides in PLHC-1 cells, while few triglycerides were accumulated in ZFL cells. Retinoic acid (cyp26b1, cyp3a65, lrata) and lipid metabolism (fasn, scd, elovl6) related genes were up-regulated by tributyltin chloride and all-trans retinoic acid, while only all-trans retinoic acid down-regulated the expression of dgat1a. The two cell models show sensitivity and responses to tributyltin chloride and all-trans retinoic acid comparable to those previously reported in mammalian cells. These results support the use of fish liver cells as alternative models for the detection of contaminants that act as lipid disrupters in the aquatic environment. |
author2 |
Ministerio de Ciencia e Innovación (España) |
author_facet |
Ministerio de Ciencia e Innovación (España) Marqueño, Anna Flores, Cintia Casado, Marta Porte Visa, Cinta |
format |
artículo |
topic_facet |
Fish cells Liver Lipid metabolism Gene expression Triglycerides |
author |
Marqueño, Anna Flores, Cintia Casado, Marta Porte Visa, Cinta |
author_sort |
Marqueño, Anna |
title |
Dysregulation of lipid metabolism in PLHC-1 and ZFL cells exposed to tributyltin an all-trans retinoic acid |
title_short |
Dysregulation of lipid metabolism in PLHC-1 and ZFL cells exposed to tributyltin an all-trans retinoic acid |
title_full |
Dysregulation of lipid metabolism in PLHC-1 and ZFL cells exposed to tributyltin an all-trans retinoic acid |
title_fullStr |
Dysregulation of lipid metabolism in PLHC-1 and ZFL cells exposed to tributyltin an all-trans retinoic acid |
title_full_unstemmed |
Dysregulation of lipid metabolism in PLHC-1 and ZFL cells exposed to tributyltin an all-trans retinoic acid |
title_sort |
dysregulation of lipid metabolism in plhc-1 and zfl cells exposed to tributyltin an all-trans retinoic acid |
publisher |
Elsevier |
publishDate |
2020-12-24 |
url |
http://hdl.handle.net/10261/226107 http://dx.doi.org/10.13039/501100004837 |
work_keys_str_mv |
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