Distribution of genes involved in sialic acid utilization in strains of Haemophilus parasuis
Haemophilus parasuis is a porcine respiratory pathogen, well known as the aetiological agent of Glässer's disease. H. parasuis comprises strains of different virulence, but the virulence factors of this bacterium are not well defined. A neuraminidase activity has been previously detected in H. parasuis, but the role of sialylation in the virulence of this bacterium has not been studied. To explore the relationship between sialic acid (Neu5Ac) and virulence, we assessed the distribution of genes involved in sialic acid metabolism in 21 H. parasuis strains from different clinical origins (including nasal and systemic isolates). The neuraminidase gene nanH, together with CMPNeu5Ac synthetase and sialyltransferase genes neuA, siaB and lsgB, were included in the study. Neuraminidase activity was found to be common in H. parasuis isolates, and the nanH gene from 12 isolates was expressed in Escherichia coli and further characterized. Sequence analysis showed that the NanH predicted protein contained the motifs characteristic of the catalytic site of sialidases. While an association between the presence of nanH and the different origins of the strains was not detected, the lsgB gene was predominantly present in the systemic isolates, and was not amplified from any of the nasal isolates tested. Analysis of the lipooligosaccharide (LOS) from reference strains Nagasaki (virulent, lsgB +) and SW114 (non-virulent, lsgB -) showed the presence of sialic acid in the LOS from the Nagasaki strain, supporting the role of sialylation in the virulence of this bacterial pathogen. Further studies are needed to clarify the role of sialic acid in the pathogenicity of H. parasuis. © 2012 SGM.
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dig-idab-es-10261-973342017-06-08T09:00:47Z Distribution of genes involved in sialic acid utilization in strains of Haemophilus parasuis Martínez, Verónica Soler-Llorens, Pedro Moleres, Javier Garmendia, Juncal Aragón, Virginia Haemophilus parasuis is a porcine respiratory pathogen, well known as the aetiological agent of Glässer's disease. H. parasuis comprises strains of different virulence, but the virulence factors of this bacterium are not well defined. A neuraminidase activity has been previously detected in H. parasuis, but the role of sialylation in the virulence of this bacterium has not been studied. To explore the relationship between sialic acid (Neu5Ac) and virulence, we assessed the distribution of genes involved in sialic acid metabolism in 21 H. parasuis strains from different clinical origins (including nasal and systemic isolates). The neuraminidase gene nanH, together with CMPNeu5Ac synthetase and sialyltransferase genes neuA, siaB and lsgB, were included in the study. Neuraminidase activity was found to be common in H. parasuis isolates, and the nanH gene from 12 isolates was expressed in Escherichia coli and further characterized. Sequence analysis showed that the NanH predicted protein contained the motifs characteristic of the catalytic site of sialidases. While an association between the presence of nanH and the different origins of the strains was not detected, the lsgB gene was predominantly present in the systemic isolates, and was not amplified from any of the nasal isolates tested. Analysis of the lipooligosaccharide (LOS) from reference strains Nagasaki (virulent, lsgB +) and SW114 (non-virulent, lsgB -) showed the presence of sialic acid in the LOS from the Nagasaki strain, supporting the role of sialylation in the virulence of this bacterial pathogen. Further studies are needed to clarify the role of sialic acid in the pathogenicity of H. parasuis. © 2012 SGM. This study was funded by the Ministerio de Ciencia e Innovación of Spain (grant AGL2010-15232) awarded to V. A., and the Gobierno de Navarra, Spain (grant IIQ14064) awarded to J. G. Peer Reviewed 2014-05-28T07:02:51Z 2014-05-28T07:02:51Z 2012-05-16 2014-05-28T07:02:51Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1099/mic.0.056994-0 issn: 1350-0872 e-issn: 1465-2080 Microbiology 158(8): 2117-2124 (2012) http://hdl.handle.net/10261/97334 10.1099/mic.0.056994-0 none Society for General Microbiology |
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Haemophilus parasuis is a porcine respiratory pathogen, well known as the aetiological agent of Glässer's disease. H. parasuis comprises strains of different virulence, but the virulence factors of this bacterium are not well defined. A neuraminidase activity has been previously detected in H. parasuis, but the role of sialylation in the virulence of this bacterium has not been studied. To explore the relationship between sialic acid (Neu5Ac) and virulence, we assessed the distribution of genes involved in sialic acid metabolism in 21 H. parasuis strains from different clinical origins (including nasal and systemic isolates). The neuraminidase gene nanH, together with CMPNeu5Ac synthetase and sialyltransferase genes neuA, siaB and lsgB, were included in the study. Neuraminidase activity was found to be common in H. parasuis isolates, and the nanH gene from 12 isolates was expressed in Escherichia coli and further characterized. Sequence analysis showed that the NanH predicted protein contained the motifs characteristic of the catalytic site of sialidases. While an association between the presence of nanH and the different origins of the strains was not detected, the lsgB gene was predominantly present in the systemic isolates, and was not amplified from any of the nasal isolates tested. Analysis of the lipooligosaccharide (LOS) from reference strains Nagasaki (virulent, lsgB +) and SW114 (non-virulent, lsgB -) showed the presence of sialic acid in the LOS from the Nagasaki strain, supporting the role of sialylation in the virulence of this bacterial pathogen. Further studies are needed to clarify the role of sialic acid in the pathogenicity of H. parasuis. © 2012 SGM. |
format |
artículo |
author |
Martínez, Verónica Soler-Llorens, Pedro Moleres, Javier Garmendia, Juncal Aragón, Virginia |
spellingShingle |
Martínez, Verónica Soler-Llorens, Pedro Moleres, Javier Garmendia, Juncal Aragón, Virginia Distribution of genes involved in sialic acid utilization in strains of Haemophilus parasuis |
author_facet |
Martínez, Verónica Soler-Llorens, Pedro Moleres, Javier Garmendia, Juncal Aragón, Virginia |
author_sort |
Martínez, Verónica |
title |
Distribution of genes involved in sialic acid utilization in strains of Haemophilus parasuis |
title_short |
Distribution of genes involved in sialic acid utilization in strains of Haemophilus parasuis |
title_full |
Distribution of genes involved in sialic acid utilization in strains of Haemophilus parasuis |
title_fullStr |
Distribution of genes involved in sialic acid utilization in strains of Haemophilus parasuis |
title_full_unstemmed |
Distribution of genes involved in sialic acid utilization in strains of Haemophilus parasuis |
title_sort |
distribution of genes involved in sialic acid utilization in strains of haemophilus parasuis |
publisher |
Society for General Microbiology |
publishDate |
2012-05-16 |
url |
http://hdl.handle.net/10261/97334 |
work_keys_str_mv |
AT martinezveronica distributionofgenesinvolvedinsialicacidutilizationinstrainsofhaemophilusparasuis AT solerllorenspedro distributionofgenesinvolvedinsialicacidutilizationinstrainsofhaemophilusparasuis AT moleresjavier distributionofgenesinvolvedinsialicacidutilizationinstrainsofhaemophilusparasuis AT garmendiajuncal distributionofgenesinvolvedinsialicacidutilizationinstrainsofhaemophilusparasuis AT aragonvirginia distributionofgenesinvolvedinsialicacidutilizationinstrainsofhaemophilusparasuis |
_version_ |
1777663438700412928 |