Identification of Spodoptera exigua nucleopolyhedrovirus genes involved in pathogenicity and virulence
Genome sequence analysis of seven different Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) isolates that differed in insecticidal phenotype permitted the identification of genes likely to be involved in pathogenicity of occlusion bodies (OBs) and speed of kill (virulence) of this virus: se4 (. hoar), se5 (unknown function), se28 (unknown function), se76 (. cg30), se87 (. p26) and se129 (. p26). To study the role of these genes experimentally on the insecticidal phenotype, a bacmid-based recombination system was constructed to delete selected genes from a SeMNPV isolate, VT-SeAL1, designated as SeBacAL1. All of the knockout viruses were viable and the repair viruses behaved like the wild-type control, vSeBacAL1. Deletion of se4, se5, se76 and se129 resulted in decreased OB pathogenicity compared to vSeBacAL1 OBs. In contrast, deletion of se87 did not significantly affect OB pathogenicity, whereas deletion of se28 resulted in significantly increased OB pathogenicity. Deletion of se4, se28, se76, se87 and se129 did not affect speed of kill compared to the bacmid vSeBacAL1, whereas speed of kill was significantly extended following deletion of se5 and in the wild-type isolate (SeAL1), compared to that of the bacmid. Therefore, biological assays confirmed that several genes had effects on virus insecticidal phenotype. Se5 is an attractive candidate gene for further studies, as it affects both biological parameters of this important biocontrol virus.
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Elsevier
2015-03
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Subjects: | Spodoptera exigua, SeMNPV, SeMNPV bacmid, Pathogenicity, Virulence, |
Online Access: | http://hdl.handle.net/10261/126445 http://dx.doi.org/10.13039/501100006280 |
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dig-idab-es-10261-1264452016-12-12T13:09:18Z Identification of Spodoptera exigua nucleopolyhedrovirus genes involved in pathogenicity and virulence Serrano, Amaya Pijlman, Gorben P. Vlak, Just M. Muñoz, Delia Williams, Trevor G. Caballero, Primitivo Ministerio de Educación y Cultura (España) Ministerio de Ciencia y Tecnología (España) Spodoptera exigua SeMNPV SeMNPV bacmid Pathogenicity Virulence Genome sequence analysis of seven different Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) isolates that differed in insecticidal phenotype permitted the identification of genes likely to be involved in pathogenicity of occlusion bodies (OBs) and speed of kill (virulence) of this virus: se4 (. hoar), se5 (unknown function), se28 (unknown function), se76 (. cg30), se87 (. p26) and se129 (. p26). To study the role of these genes experimentally on the insecticidal phenotype, a bacmid-based recombination system was constructed to delete selected genes from a SeMNPV isolate, VT-SeAL1, designated as SeBacAL1. All of the knockout viruses were viable and the repair viruses behaved like the wild-type control, vSeBacAL1. Deletion of se4, se5, se76 and se129 resulted in decreased OB pathogenicity compared to vSeBacAL1 OBs. In contrast, deletion of se87 did not significantly affect OB pathogenicity, whereas deletion of se28 resulted in significantly increased OB pathogenicity. Deletion of se4, se28, se76, se87 and se129 did not affect speed of kill compared to the bacmid vSeBacAL1, whereas speed of kill was significantly extended following deletion of se5 and in the wild-type isolate (SeAL1), compared to that of the bacmid. Therefore, biological assays confirmed that several genes had effects on virus insecticidal phenotype. Se5 is an attractive candidate gene for further studies, as it affects both biological parameters of this important biocontrol virus. This study received financial support from the Spanish Ministry for Science and Technology (AGL2008-05456-C03-01). A.S. received a predoctoral fellowship (BES-2009-012043) from the Spanish Ministry of Education and Culture. Peer Reviewed 2015-12-03T10:01:14Z 2015-12-03T10:01:14Z 2015-03 2015-12-03T10:01:14Z artículo http://purl.org/coar/resource_type/c_6501 issn: 1096-0805 Journal of Invertebrate Pathology 126: 43-50 (2015) http://hdl.handle.net/10261/126445 10.1016/j.jip.2015.01.008 http://dx.doi.org/10.13039/501100006280 Postprint http://dx.doi.org/10.1016/j.jip.2015.01.008 Sí none Elsevier |
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Spodoptera exigua SeMNPV SeMNPV bacmid Pathogenicity Virulence Spodoptera exigua SeMNPV SeMNPV bacmid Pathogenicity Virulence |
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Spodoptera exigua SeMNPV SeMNPV bacmid Pathogenicity Virulence Spodoptera exigua SeMNPV SeMNPV bacmid Pathogenicity Virulence Serrano, Amaya Pijlman, Gorben P. Vlak, Just M. Muñoz, Delia Williams, Trevor G. Caballero, Primitivo Identification of Spodoptera exigua nucleopolyhedrovirus genes involved in pathogenicity and virulence |
description |
Genome sequence analysis of seven different Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) isolates that differed in insecticidal phenotype permitted the identification of genes likely to be involved in pathogenicity of occlusion bodies (OBs) and speed of kill (virulence) of this virus: se4 (. hoar), se5 (unknown function), se28 (unknown function), se76 (. cg30), se87 (. p26) and se129 (. p26). To study the role of these genes experimentally on the insecticidal phenotype, a bacmid-based recombination system was constructed to delete selected genes from a SeMNPV isolate, VT-SeAL1, designated as SeBacAL1. All of the knockout viruses were viable and the repair viruses behaved like the wild-type control, vSeBacAL1. Deletion of se4, se5, se76 and se129 resulted in decreased OB pathogenicity compared to vSeBacAL1 OBs. In contrast, deletion of se87 did not significantly affect OB pathogenicity, whereas deletion of se28 resulted in significantly increased OB pathogenicity. Deletion of se4, se28, se76, se87 and se129 did not affect speed of kill compared to the bacmid vSeBacAL1, whereas speed of kill was significantly extended following deletion of se5 and in the wild-type isolate (SeAL1), compared to that of the bacmid. Therefore, biological assays confirmed that several genes had effects on virus insecticidal phenotype. Se5 is an attractive candidate gene for further studies, as it affects both biological parameters of this important biocontrol virus. |
author2 |
Ministerio de Educación y Cultura (España) |
author_facet |
Ministerio de Educación y Cultura (España) Serrano, Amaya Pijlman, Gorben P. Vlak, Just M. Muñoz, Delia Williams, Trevor G. Caballero, Primitivo |
format |
artículo |
topic_facet |
Spodoptera exigua SeMNPV SeMNPV bacmid Pathogenicity Virulence |
author |
Serrano, Amaya Pijlman, Gorben P. Vlak, Just M. Muñoz, Delia Williams, Trevor G. Caballero, Primitivo |
author_sort |
Serrano, Amaya |
title |
Identification of Spodoptera exigua nucleopolyhedrovirus genes involved in pathogenicity and virulence |
title_short |
Identification of Spodoptera exigua nucleopolyhedrovirus genes involved in pathogenicity and virulence |
title_full |
Identification of Spodoptera exigua nucleopolyhedrovirus genes involved in pathogenicity and virulence |
title_fullStr |
Identification of Spodoptera exigua nucleopolyhedrovirus genes involved in pathogenicity and virulence |
title_full_unstemmed |
Identification of Spodoptera exigua nucleopolyhedrovirus genes involved in pathogenicity and virulence |
title_sort |
identification of spodoptera exigua nucleopolyhedrovirus genes involved in pathogenicity and virulence |
publisher |
Elsevier |
publishDate |
2015-03 |
url |
http://hdl.handle.net/10261/126445 http://dx.doi.org/10.13039/501100006280 |
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