Toward developing recombinant gonadotropin-based hormone therapies for increasing fertility in the flatfish Senegalese sole

Chauvigné, François; Ollé, Judith; González, Wendy; Duncan, Neil; Giménez, Ignacio; Cerdà, Joan; Consejo Nacional de Ciencia y Tecnología (México)

Toward developing recombinant gonadotropin-based hormone therapies for increasing fertility in the flatfish Senegalese sole

27 pages, 10 figures, supporting information https://doi.org/10.1371/journal.pone.0174387.s001 https://doi.org/10.1371/journal.pone.0174387.s002

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Main Authors:	Chauvigné, François, Ollé, Judith, González, Wendy, Duncan, Neil, Giménez, Ignacio, Cerdà, Joan
Other Authors:	Consejo Nacional de Ciencia y Tecnología (México)
Format:	artículo biblioteca
Published:	Public Library of Science 2017-03
Online Access:	http://hdl.handle.net/10261/151518 http://dx.doi.org/10.13039/501100003141 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/501100002809 http://dx.doi.org/10.13039/501100003329
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spelling	dig-icm-es-10261-1515182021-12-28T16:19:50Z Toward developing recombinant gonadotropin-based hormone therapies for increasing fertility in the flatfish Senegalese sole Chauvigné, François Ollé, Judith González, Wendy Duncan, Neil Giménez, Ignacio Cerdà, Joan Consejo Nacional de Ciencia y Tecnología (México) European Commission CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Generalitat de Catalunya Ministerio de Economía y Competitividad (España) 27 pages, 10 figures, supporting information https://doi.org/10.1371/journal.pone.0174387.s001 https://doi.org/10.1371/journal.pone.0174387.s002 Captive flatfishes, such as the Senegalese sole, typically produce very low volumes of sperm. This situation is particularly prevalent in the first generation (F1) of reared sole males, which limits the development of artificial fertilization methods and the implementation of selective breeding programs. In this study, we investigated whether combined treatments with homologous recombinant follicle-stimulating (rFsh) and luteinizing (rLh) hormones, produced in a mammalian host system, could stimulate spermatogenesis and enhance sperm production in Senegalese sole F1 males. In an initial autumn/winter experiment, weekly intramuscular injections with increasing doses of rFsh over 9 weeks resulted in the stimulation of gonad weight, androgen release, germ cell proliferation and entry into meiosis, and the expression of different spermatogenesis-related genes, whereas a subsequent single rLh injection potentiated spermatozoa differentiation. In a second late winter/spring trial corresponding to the sole’s natural prespawning and spawning periods, we tested the effect of repeated rLh injections on the amount and quality of sperm produced by males previously treated with rFsh for 4, 6, 8 or 10 weeks. These latter results showed that the combination of rFsh and rLh treatments could increase sperm production up to 7 times, and slightly improve the motility of the spermatozoa, although a high variability in the response was found. However, sustained administration of rFsh during spawning markedly diminished Leydig cell survival and the steroidogenic potential of the testis. These data suggest that in vivo application of rFsh and rLh is effective at stimulating spermatogenesis and sperm production in Senegalese sole F1 males, setting the basis for the future establishment of recombinant gonadotropin-based hormone therapies to ameliorate reproductive dysfunctions of this species This work was supported by the CERCA Programme (Government of Catalonia), and partially funded by the Spanish Ministry of Economy and Competitivity (MINECO) (Grant no. AGL2013-41196-R to JC), and the Spanish National Institute for Agronomic Research (INIA)-European Fund for Economic and Regional Development (FEDER) (Grant no. RTA2014-00048 to ND). Participation of FC and WG was funded, respectively, by a >Ramon y Cajal> contract (MINECO) and a predoctoral grant from the National Board of Science and Technology (CONACYT, Mexico) Peer Reviewed 2017-06-16T07:21:43Z 2017-06-16T07:21:43Z 2017-03 2017-06-16T07:21:43Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1371/journal.pone.0174387 e-issn: 1932-6203 PLoS ONE 12(3): e0174387 (2017) http://hdl.handle.net/10261/151518 10.1371/journal.pone.0174387 http://dx.doi.org/10.13039/501100003141 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/501100002809 http://dx.doi.org/10.13039/501100003329 28329024 Publisher's version https://doi.org/10.1371/journal.pone.0174387 Sí open Public Library of Science
institution	ICM ES
collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-icm-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del ICM España
description	27 pages, 10 figures, supporting information https://doi.org/10.1371/journal.pone.0174387.s001 https://doi.org/10.1371/journal.pone.0174387.s002
author2	Consejo Nacional de Ciencia y Tecnología (México)
author_facet	Consejo Nacional de Ciencia y Tecnología (México) Chauvigné, François Ollé, Judith González, Wendy Duncan, Neil Giménez, Ignacio Cerdà, Joan
format	artículo
author	Chauvigné, François Ollé, Judith González, Wendy Duncan, Neil Giménez, Ignacio Cerdà, Joan
spellingShingle	Chauvigné, François Ollé, Judith González, Wendy Duncan, Neil Giménez, Ignacio Cerdà, Joan Toward developing recombinant gonadotropin-based hormone therapies for increasing fertility in the flatfish Senegalese sole
author_sort	Chauvigné, François
title	Toward developing recombinant gonadotropin-based hormone therapies for increasing fertility in the flatfish Senegalese sole
title_short	Toward developing recombinant gonadotropin-based hormone therapies for increasing fertility in the flatfish Senegalese sole
title_full	Toward developing recombinant gonadotropin-based hormone therapies for increasing fertility in the flatfish Senegalese sole
title_fullStr	Toward developing recombinant gonadotropin-based hormone therapies for increasing fertility in the flatfish Senegalese sole
title_full_unstemmed	Toward developing recombinant gonadotropin-based hormone therapies for increasing fertility in the flatfish Senegalese sole
title_sort	toward developing recombinant gonadotropin-based hormone therapies for increasing fertility in the flatfish senegalese sole
publisher	Public Library of Science
publishDate	2017-03
url	http://hdl.handle.net/10261/151518 http://dx.doi.org/10.13039/501100003141 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/501100002809 http://dx.doi.org/10.13039/501100003329
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Toward developing recombinant gonadotropin-based hormone therapies for increasing fertility in the flatfish Senegalese sole

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