Flavodoxin overexpression confers tolerance to oxidative stress in beneficial soil bacteria and improves survival in the presence of the herbicides paraquat and atrazine
11 páginas y 4 figuras
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Society for Applied Microbiology
2013
|
| Subjects: | Atrazine, Ensifer meliloti, Flavodoxin, Oxidative stress, Paraquat, Pseudomonas fluorescens, |
| Online Access: | http://hdl.handle.net/10261/78648 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-ica-es-10261-78648 |
|---|---|
| record_format |
koha |
| spelling |
dig-ica-es-10261-786482022-06-10T09:21:04Z Flavodoxin overexpression confers tolerance to oxidative stress in beneficial soil bacteria and improves survival in the presence of the herbicides paraquat and atrazine Coba de la Peña, Teodoro Redondo, F. J. Fillat, María F. Lucas, M. Mercedes Pueyo, José Javier Atrazine Ensifer meliloti Flavodoxin Oxidative stress Paraquat Pseudomonas fluorescens 11 páginas y 4 figuras Aim: To determine whether expression of a cyanobacterial flavodoxin in soil bacteria of agronomic interest confers protection against the widely used herbicides paraquat and atrazine. Methods and Results: The model bacterium Escherichia coli, the symbiotic nitrogen-fixing bacterium Ensifer meliloti and the plant growth-promoting rhizobacterium Pseudomonas fluorescens Aur6 were transformed with expression vectors containing the flavodoxin gene of Anabaena variabilis. Expression of the cyanobacterial protein was confirmed by Western blot. Bacterial tolerance to oxidative stress was tested in solid medium supplemented with hydrogen peroxide, paraquat or atrazine. In all three bacterial strains, flavodoxin expression enhanced tolerance to the oxidative stress provoked by hydrogen peroxide and by the reactive oxygen species-inducing herbicides, witnessed by the enhanced survival of the transformed bacteria in the presence of these oxidizing agents. Conclusions: Flavodoxin overexpression in beneficial soil bacteria confers tolerance to oxidative stress and improves their survival in the presence of the herbicides paraquat and atrazine. Flavodoxin could be considered as a general antioxidant resource to face oxidative challenges in different micro-organisms. Significance and Impact of the study: The use of plant growth-promoting rhizobacteria or nitrogen-fixing bacteria with enhanced tolerance to oxidative stress in contaminated soils is of significant agronomic interest. The enhanced tolerance of flavodoxin-expressing bacteria to atrazine and paraquat points to potential applications in herbicide-treated soils. Peer reviewed 2013-06-26T10:49:49Z 2013-06-26T10:49:49Z 2013 artículo http://purl.org/coar/resource_type/c_6501 Journal of Applied Microbiology 115: 236--246 (2013) http://hdl.handle.net/10261/78648 10.1111/jam.12224 en #PLACEHOLDER_PARENT_METADATA_VALUE# S2009/AMB-1511/MICROAMBIENTE http://dx.doi.org/10.1111/jam.12224 none Society for Applied Microbiology |
| institution |
ICA ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-ica-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del ICA España |
| language |
English |
| topic |
Atrazine Ensifer meliloti Flavodoxin Oxidative stress Paraquat Pseudomonas fluorescens Atrazine Ensifer meliloti Flavodoxin Oxidative stress Paraquat Pseudomonas fluorescens |
| spellingShingle |
Atrazine Ensifer meliloti Flavodoxin Oxidative stress Paraquat Pseudomonas fluorescens Atrazine Ensifer meliloti Flavodoxin Oxidative stress Paraquat Pseudomonas fluorescens Coba de la Peña, Teodoro Redondo, F. J. Fillat, María F. Lucas, M. Mercedes Pueyo, José Javier Flavodoxin overexpression confers tolerance to oxidative stress in beneficial soil bacteria and improves survival in the presence of the herbicides paraquat and atrazine |
| description |
11 páginas y 4 figuras |
| format |
artículo |
| topic_facet |
Atrazine Ensifer meliloti Flavodoxin Oxidative stress Paraquat Pseudomonas fluorescens |
| author |
Coba de la Peña, Teodoro Redondo, F. J. Fillat, María F. Lucas, M. Mercedes Pueyo, José Javier |
| author_facet |
Coba de la Peña, Teodoro Redondo, F. J. Fillat, María F. Lucas, M. Mercedes Pueyo, José Javier |
| author_sort |
Coba de la Peña, Teodoro |
| title |
Flavodoxin overexpression confers tolerance to oxidative stress in beneficial soil bacteria and improves survival in the presence of the herbicides paraquat and atrazine |
| title_short |
Flavodoxin overexpression confers tolerance to oxidative stress in beneficial soil bacteria and improves survival in the presence of the herbicides paraquat and atrazine |
| title_full |
Flavodoxin overexpression confers tolerance to oxidative stress in beneficial soil bacteria and improves survival in the presence of the herbicides paraquat and atrazine |
| title_fullStr |
Flavodoxin overexpression confers tolerance to oxidative stress in beneficial soil bacteria and improves survival in the presence of the herbicides paraquat and atrazine |
| title_full_unstemmed |
Flavodoxin overexpression confers tolerance to oxidative stress in beneficial soil bacteria and improves survival in the presence of the herbicides paraquat and atrazine |
| title_sort |
flavodoxin overexpression confers tolerance to oxidative stress in beneficial soil bacteria and improves survival in the presence of the herbicides paraquat and atrazine |
| publisher |
Society for Applied Microbiology |
| publishDate |
2013 |
| url |
http://hdl.handle.net/10261/78648 |
| work_keys_str_mv |
AT cobadelapenateodoro flavodoxinoverexpressionconferstolerancetooxidativestressinbeneficialsoilbacteriaandimprovessurvivalinthepresenceoftheherbicidesparaquatandatrazine AT redondofj flavodoxinoverexpressionconferstolerancetooxidativestressinbeneficialsoilbacteriaandimprovessurvivalinthepresenceoftheherbicidesparaquatandatrazine AT fillatmariaf flavodoxinoverexpressionconferstolerancetooxidativestressinbeneficialsoilbacteriaandimprovessurvivalinthepresenceoftheherbicidesparaquatandatrazine AT lucasmmercedes flavodoxinoverexpressionconferstolerancetooxidativestressinbeneficialsoilbacteriaandimprovessurvivalinthepresenceoftheherbicidesparaquatandatrazine AT pueyojosejavier flavodoxinoverexpressionconferstolerancetooxidativestressinbeneficialsoilbacteriaandimprovessurvivalinthepresenceoftheherbicidesparaquatandatrazine |
| _version_ |
1777663617989083136 |