DIPA-CRISPR is a simple and accessible method for insect gene editing

Current approaches for insect gene editing require microinjection of materials into early embryos. This severely limits the application of gene editing to a great number of insect species, especially to those whose reproduction systems preclude access to early embryos for injection. To overcome these limitations, we report a simple and accessible method for insect gene editing, termed “direct parental” CRISPR (DIPA-CRISPR). We show that injection of Cas9 ribonucleoproteins (RNPs) into the haemocoel of adult females efficiently introduces heritable mutations in developing oocytes. Importantly, commercially available standard Cas9 protein can be directly used for DIPA-CRISPR, which makes this approach highly practical and feasible. DIPA-CRISPR enables highly efficient gene editing in the cockroaches, on which conventional approaches cannot be applied, and in the model beetle Tribolium castaneum. Due to its simplicity and accessibility, DIPA-CRISPR will greatly extend the application of gene editing technology to a wide variety of insects.

Bibliographic Details

Main Authors:	Shirai, Yu, Piulachs, Maria-Dolors, Bellés, Xavier, Daimon, Takaaki
Other Authors:	Japan Society for the Promotion of Science
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2022-05-23
Subjects:	CRISPR-Cas9, DIPA-CRISPR, Gene editing, Genome editing, Cockroaches, Beetles,
Online Access:	http://hdl.handle.net/10261/272293 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100001691 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003993
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