Experimental evolution of an emerging plant virus in host genotypes that differ in their susceptibility to infection
This study evaluates the extent to which genetic differences among host individuals from the same species condition the evolution of a plant RNA virus. We performed a threefold replicated evolution experiment in which Tobacco etch potyvirus isolate At17b (TEV-At17b), adapted to Arabidopsis thaliana ecotype Ler-0, was serially passaged in five genetically heterogeneous ecotypes of A. thaliana. After 15 passages we found that evolved viruses improved their fitness, showed higher infectivity and stronger virulence in their local host ecotypes. The genome of evolved lineages was sequenced and putative adaptive mutations identified. Host-driven convergent mutations have been identified. Evidences supported selection for increased translational efficiency. Next, we sought for the specificity of virus adaptation by infecting all five ecotypes with all 15 evolved virus populations. We found that some ecotypes were more permissive to infection than others, and that some evolved virus isolates were more specialist/generalist than others. The bipartite network linking ecotypes with evolved viruses was significantly nested but not modular, suggesting that hard-to-infect ecotypes were infected by generalist viruses whereas easy-to-infect ecotypes were infected by all viruses, as predicted by a gene-for-gene model of infection.
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Wiley-Blackwell
2014-08-01
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Subjects: | Emerging viruses, Experimental evolution, Generalists, Virus evolution, |
Online Access: | http://hdl.handle.net/10261/108818 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 |
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dig-ibe-es-10261-1088182018-10-03T10:17:11Z Experimental evolution of an emerging plant virus in host genotypes that differ in their susceptibility to infection Hillung, Julia Cuevas, José M. Valverde, Sergi Elena, Santiago F. Ministerio de Economía y Competitividad (España) European Commission Consejo Superior de Investigaciones Científicas (España) Emerging viruses Experimental evolution Generalists Virus evolution This study evaluates the extent to which genetic differences among host individuals from the same species condition the evolution of a plant RNA virus. We performed a threefold replicated evolution experiment in which Tobacco etch potyvirus isolate At17b (TEV-At17b), adapted to Arabidopsis thaliana ecotype Ler-0, was serially passaged in five genetically heterogeneous ecotypes of A. thaliana. After 15 passages we found that evolved viruses improved their fitness, showed higher infectivity and stronger virulence in their local host ecotypes. The genome of evolved lineages was sequenced and putative adaptive mutations identified. Host-driven convergent mutations have been identified. Evidences supported selection for increased translational efficiency. Next, we sought for the specificity of virus adaptation by infecting all five ecotypes with all 15 evolved virus populations. We found that some ecotypes were more permissive to infection than others, and that some evolved virus isolates were more specialist/generalist than others. The bipartite network linking ecotypes with evolved viruses was significantly nested but not modular, suggesting that hard-to-infect ecotypes were infected by generalist viruses whereas easy-to-infect ecotypes were infected by all viruses, as predicted by a gene-for-gene model of infection. We acknowledge grant BFU2012–30805 from the Spanish Ministerio de Economía y Competitividad to SFE. JMC was supported by a JAE-doc contract from CSIC. JH was supported by a pre-doctoral fellowship from Ministerio de Economía y Competitividad Peer reviewed 2014-12-17T09:24:01Z 2014-12-17T09:24:01Z 2014-08-01 artículo http://purl.org/coar/resource_type/c_6501 Evolution 68: 2467-2480 (2014) 0014-3820 http://hdl.handle.net/10261/108818 10.1111/evo.12458 1558-5646 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 en Postprint http://dx.doi.org/ 10.1111/evo.12458 Sí open Wiley-Blackwell |
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Emerging viruses Experimental evolution Generalists Virus evolution Emerging viruses Experimental evolution Generalists Virus evolution Hillung, Julia Cuevas, José M. Valverde, Sergi Elena, Santiago F. Experimental evolution of an emerging plant virus in host genotypes that differ in their susceptibility to infection |
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This study evaluates the extent to which genetic differences among host individuals from the same species condition the evolution of a plant RNA virus. We performed a threefold replicated evolution experiment in which Tobacco etch potyvirus isolate At17b (TEV-At17b), adapted to Arabidopsis thaliana ecotype Ler-0, was serially passaged in five genetically heterogeneous ecotypes of A. thaliana. After 15 passages we found that evolved viruses improved their fitness, showed higher infectivity and stronger virulence in their local host ecotypes. The genome of evolved lineages was sequenced and putative adaptive mutations identified. Host-driven convergent mutations have been identified. Evidences supported selection for increased translational efficiency. Next, we sought for the specificity of virus adaptation by infecting all five ecotypes with all 15 evolved virus populations. We found that some ecotypes were more permissive to infection than others, and that some evolved virus isolates were more specialist/generalist than others. The bipartite network linking ecotypes with evolved viruses was significantly nested but not modular, suggesting that hard-to-infect ecotypes were infected by generalist viruses whereas easy-to-infect ecotypes were infected by all viruses, as predicted by a gene-for-gene model of infection. |
author2 |
Ministerio de Economía y Competitividad (España) |
author_facet |
Ministerio de Economía y Competitividad (España) Hillung, Julia Cuevas, José M. Valverde, Sergi Elena, Santiago F. |
format |
artículo |
topic_facet |
Emerging viruses Experimental evolution Generalists Virus evolution |
author |
Hillung, Julia Cuevas, José M. Valverde, Sergi Elena, Santiago F. |
author_sort |
Hillung, Julia |
title |
Experimental evolution of an emerging plant virus in host genotypes that differ in their susceptibility to infection |
title_short |
Experimental evolution of an emerging plant virus in host genotypes that differ in their susceptibility to infection |
title_full |
Experimental evolution of an emerging plant virus in host genotypes that differ in their susceptibility to infection |
title_fullStr |
Experimental evolution of an emerging plant virus in host genotypes that differ in their susceptibility to infection |
title_full_unstemmed |
Experimental evolution of an emerging plant virus in host genotypes that differ in their susceptibility to infection |
title_sort |
experimental evolution of an emerging plant virus in host genotypes that differ in their susceptibility to infection |
publisher |
Wiley-Blackwell |
publishDate |
2014-08-01 |
url |
http://hdl.handle.net/10261/108818 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 |
work_keys_str_mv |
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