Phytohormones in the Penicillium digitatum-citrus fruit interaction
Early- and long-term variations in indole-3-acetic acid (IAA), salicylic acid (SA), and jasmonic acid (JA)-related metabolites occurring during Penicillium. digitatum infection in the flavedo (outer peel part) and albedo (inner peel part) of Navelate orange (Citrus sinensis (L.) Osbeck), and in the flavedo of its mutant Pinalate, which is deficient in abscisic acid (ABA), were compared. The levels of these metabolites were also examined in the fungal spores either obtained from in vitro grown cultures or isolated from infected fruit. The hormones were determined by ultra-high performance chromatography (UHPLC) connected to a Qtrap triple quadrupole mass spectrometer. The results showed that IAA barely changed either in the albedo of Navelate or in the flavedo of the mutant, but sharply and transiently increased in the flavedo of the parental. This highlight the relevance of IAA in very early infection perception. Moreover, results showed that IAA levels were higher in zones clearly showing mycelium or spores than in the healthy or macerated zones. They also indicate that SA participates in the initial signalling of both the flavedo and the albedo upon fungal attack and demonstrated the relevance of the interaction between this hormone and ABA in the citrus fruit-P. digitatum interaction. The concentration of cis-(+)− 12-oxo-phytodienoic acid (OPDA) dropped during the initiation of maceration and at early infection stages. The fungus redirected jasmonate metabolism towards the synthesis of methyl jasmonate (MeJA) and jasmonoyl-isoleucine (JAIle) from OPDA in a later infection stage. Finally, the results demonstrated for the first time that P. digitatum is able of producing SA, IAA, OPDA, JA, MeJA and JAIle, and that the fungus’ ability to produce OPDA was reduced, and that of IAA and JA stimulated, because of the fruit influence.
Main Authors: | , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
Elsevier
2023-12-14
|
Subjects: | Abscisic acid, Albedo/flavedo, Fungal hormones, Indole-3-acetic acid, Jasmonates, Salicylic acid, |
Online Access: | http://hdl.handle.net/10261/341745 https://api.elsevier.com/content/abstract/scopus_id/85179992341 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-iata-es-10261-341745 |
---|---|
record_format |
koha |
institution |
IATA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-iata-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del IATA España |
language |
English |
topic |
Abscisic acid Albedo/flavedo Fungal hormones Indole-3-acetic acid Jasmonates Salicylic acid Abscisic acid Albedo/flavedo Fungal hormones Indole-3-acetic acid Jasmonates Salicylic acid |
spellingShingle |
Abscisic acid Albedo/flavedo Fungal hormones Indole-3-acetic acid Jasmonates Salicylic acid Abscisic acid Albedo/flavedo Fungal hormones Indole-3-acetic acid Jasmonates Salicylic acid Lafuente, María Teresa González-Candelas, Luis Phytohormones in the Penicillium digitatum-citrus fruit interaction |
description |
Early- and long-term variations in indole-3-acetic acid (IAA), salicylic acid (SA), and jasmonic acid (JA)-related metabolites occurring during Penicillium. digitatum infection in the flavedo (outer peel part) and albedo (inner peel part) of Navelate orange (Citrus sinensis (L.) Osbeck), and in the flavedo of its mutant Pinalate, which is deficient in abscisic acid (ABA), were compared. The levels of these metabolites were also examined in the fungal spores either obtained from in vitro grown cultures or isolated from infected fruit. The hormones were determined by ultra-high performance chromatography (UHPLC) connected to a Qtrap triple quadrupole mass spectrometer. The results showed that IAA barely changed either in the albedo of Navelate or in the flavedo of the mutant, but sharply and transiently increased in the flavedo of the parental. This highlight the relevance of IAA in very early infection perception. Moreover, results showed that IAA levels were higher in zones clearly showing mycelium or spores than in the healthy or macerated zones. They also indicate that SA participates in the initial signalling of both the flavedo and the albedo upon fungal attack and demonstrated the relevance of the interaction between this hormone and ABA in the citrus fruit-P. digitatum interaction. The concentration of cis-(+)− 12-oxo-phytodienoic acid (OPDA) dropped during the initiation of maceration and at early infection stages. The fungus redirected jasmonate metabolism towards the synthesis of methyl jasmonate (MeJA) and jasmonoyl-isoleucine (JAIle) from OPDA in a later infection stage. Finally, the results demonstrated for the first time that P. digitatum is able of producing SA, IAA, OPDA, JA, MeJA and JAIle, and that the fungus’ ability to produce OPDA was reduced, and that of IAA and JA stimulated, because of the fruit influence. |
author2 |
Agencia Estatal de Investigación (España) |
author_facet |
Agencia Estatal de Investigación (España) Lafuente, María Teresa González-Candelas, Luis |
format |
artículo |
topic_facet |
Abscisic acid Albedo/flavedo Fungal hormones Indole-3-acetic acid Jasmonates Salicylic acid |
author |
Lafuente, María Teresa González-Candelas, Luis |
author_sort |
Lafuente, María Teresa |
title |
Phytohormones in the Penicillium digitatum-citrus fruit interaction |
title_short |
Phytohormones in the Penicillium digitatum-citrus fruit interaction |
title_full |
Phytohormones in the Penicillium digitatum-citrus fruit interaction |
title_fullStr |
Phytohormones in the Penicillium digitatum-citrus fruit interaction |
title_full_unstemmed |
Phytohormones in the Penicillium digitatum-citrus fruit interaction |
title_sort |
phytohormones in the penicillium digitatum-citrus fruit interaction |
publisher |
Elsevier |
publishDate |
2023-12-14 |
url |
http://hdl.handle.net/10261/341745 https://api.elsevier.com/content/abstract/scopus_id/85179992341 |
work_keys_str_mv |
AT lafuentemariateresa phytohormonesinthepenicilliumdigitatumcitrusfruitinteraction AT gonzalezcandelasluis phytohormonesinthepenicilliumdigitatumcitrusfruitinteraction |
_version_ |
1792484865320419328 |
spelling |
dig-iata-es-10261-3417452024-01-05T21:51:27Z Phytohormones in the Penicillium digitatum-citrus fruit interaction Lafuente, María Teresa González-Candelas, Luis Agencia Estatal de Investigación (España) Consejo Superior de Investigaciones Científicas (España) European Commission #NODATA# #NODATA# Abscisic acid Albedo/flavedo Fungal hormones Indole-3-acetic acid Jasmonates Salicylic acid Early- and long-term variations in indole-3-acetic acid (IAA), salicylic acid (SA), and jasmonic acid (JA)-related metabolites occurring during Penicillium. digitatum infection in the flavedo (outer peel part) and albedo (inner peel part) of Navelate orange (Citrus sinensis (L.) Osbeck), and in the flavedo of its mutant Pinalate, which is deficient in abscisic acid (ABA), were compared. The levels of these metabolites were also examined in the fungal spores either obtained from in vitro grown cultures or isolated from infected fruit. The hormones were determined by ultra-high performance chromatography (UHPLC) connected to a Qtrap triple quadrupole mass spectrometer. The results showed that IAA barely changed either in the albedo of Navelate or in the flavedo of the mutant, but sharply and transiently increased in the flavedo of the parental. This highlight the relevance of IAA in very early infection perception. Moreover, results showed that IAA levels were higher in zones clearly showing mycelium or spores than in the healthy or macerated zones. They also indicate that SA participates in the initial signalling of both the flavedo and the albedo upon fungal attack and demonstrated the relevance of the interaction between this hormone and ABA in the citrus fruit-P. digitatum interaction. The concentration of cis-(+)− 12-oxo-phytodienoic acid (OPDA) dropped during the initiation of maceration and at early infection stages. The fungus redirected jasmonate metabolism towards the synthesis of methyl jasmonate (MeJA) and jasmonoyl-isoleucine (JAIle) from OPDA in a later infection stage. Finally, the results demonstrated for the first time that P. digitatum is able of producing SA, IAA, OPDA, JA, MeJA and JAIle, and that the fungus’ ability to produce OPDA was reduced, and that of IAA and JA stimulated, because of the fruit influence. This work was funded by Projects AGL2017-88120-R and PID2021-126005OB-I00 (MCIU/AEI/FEDER, UE) and 2020AEPP135 (MCIU/CSIC). We acknowledge the award of the Spanish government MCIN/AEI to the IATA-CSIC as Center of Excellence Accreditation Severo Ochoa (CEX2021-001189-S/ MCIN/AEI / 10.13039/501100011033). We also thank the SCSIE-UV Chromatography Facility for the technical support and for allowing us the use of instruments. Moreover, we acknowledge the help of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX 2021-001189-S) Peer reviewed 2024-01-05T12:24:17Z 2024-01-05T12:24:17Z 2023-12-14 artículo Postharvest Biology and Technology 209: 112704 (2024) 0925-5214 CEX 2021-001189-S http://hdl.handle.net/10261/341745 10.1016/j.postharvbio.2023.112704 2-s2.0-85179992341 https://api.elsevier.com/content/abstract/scopus_id/85179992341 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88120-R/ES/MECANISMOS DE PATOGENICIDAD Y DE RESISTENCIA A LA INFECCION EN LA INTERACCION FRUTO CITRICO-PENICILLIUM DIGITATUM/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-126005OB-I00 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX2021-001189-S info:eu-repo/grantAgreement/MCIU/CSIC/2020AEPP135 Postharvest Biology and Technology Publisher's version https://doi.org/10.1016/j.postharvbio.2023.112704 Sí open Elsevier |