Modulation of AggR levels reveals features of virulence regulation in enteroaggregative E. coli
Enteroaggregative Escherichia coli (EAEC) strains are one of the diarrheagenic pathotypes. EAEC strains harbor a virulence plasmid (pAA2) that encodes, among other virulence determinants, the aggR gene. The expression of the AggR protein leads to the expression of several virulence determinants in both plasmids and chromosomes. In this work, we describe a novel mechanism that influences AggR expression. Because of the absence of a Rho-independent terminator in the 3'UTR, aggR transcripts extend far beyond the aggR ORF. These transcripts are prone to PNPase-mediated degradation. Structural alterations in the 3'UTR result in increased aggR transcript stability, leading to increased AggR levels. We therefore investigated the effect of increased AggR levels on EAEC virulence. Upon finding the previously described AggR-dependent virulence factors, we detected novel AggR-regulated genes that may play relevant roles in EAEC virulence. Mutants exhibiting high AggR levels because of structural alterations in the aggR 3'UTR show increased mobility and increased pAA2 conjugation frequency. Furthermore, among the genes exhibiting increased fold change values, we could identify those of metabolic pathways that promote increased degradation of arginine, fatty acids and gamma-aminobutyric acid (GABA), respectively. In this paper, we discuss how the AggR-dependent increase in specific metabolic pathways activity may contribute to EAEC virulence.
Auteurs principaux: | , , , , , , , , |
---|---|
Autres auteurs: | |
Format: | artículo biblioteca |
Langue: | English |
Publié: |
Springer Nature
2021-11-16
|
Sujets: | Microbial genetics, EAEC virulence, AggR expression, |
Accès en ligne: | http://hdl.handle.net/10261/256154 http://dx.doi.org/10.13039/501100002809 http://dx.doi.org/10.13039/501100003329 https://api.elsevier.com/content/abstract/scopus_id/85119104837 |
Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|
id |
dig-iata-es-10261-256154 |
---|---|
record_format |
koha |
spelling |
dig-iata-es-10261-2561542023-02-08T21:35:48Z Modulation of AggR levels reveals features of virulence regulation in enteroaggregative E. coli Prieto, Alejandro Bernabeu, Manuel Sánchez Herrero, José Francisco Pérez Bosque, Anna Miró, Lluïsa Bäuerl, Christine Collado, María Carmen Hüttener, Mário Juárez, Antonio Generalitat de Catalunya Ministerio de Economía y Competitividad (España) Ministerio de Ciencia, Innovación y Universidades (España) 0000-0002-9175-5809 0000-0001-6771-4807 0000-0003-2175-9684 0000-0002-6204-4864 0000-0002-9155-6084 0000-0002-5027-8778 Microbial genetics EAEC virulence AggR expression Enteroaggregative Escherichia coli (EAEC) strains are one of the diarrheagenic pathotypes. EAEC strains harbor a virulence plasmid (pAA2) that encodes, among other virulence determinants, the aggR gene. The expression of the AggR protein leads to the expression of several virulence determinants in both plasmids and chromosomes. In this work, we describe a novel mechanism that influences AggR expression. Because of the absence of a Rho-independent terminator in the 3'UTR, aggR transcripts extend far beyond the aggR ORF. These transcripts are prone to PNPase-mediated degradation. Structural alterations in the 3'UTR result in increased aggR transcript stability, leading to increased AggR levels. We therefore investigated the effect of increased AggR levels on EAEC virulence. Upon finding the previously described AggR-dependent virulence factors, we detected novel AggR-regulated genes that may play relevant roles in EAEC virulence. Mutants exhibiting high AggR levels because of structural alterations in the aggR 3'UTR show increased mobility and increased pAA2 conjugation frequency. Furthermore, among the genes exhibiting increased fold change values, we could identify those of metabolic pathways that promote increased degradation of arginine, fatty acids and gamma-aminobutyric acid (GABA), respectively. In this paper, we discuss how the AggR-dependent increase in specific metabolic pathways activity may contribute to EAEC virulence. This work was supported by grants BIO2016-76412-C2-1-R and PID2019-107479RB-I00 (AEI/FEDER, UE) and the Ministerio de Economía, Industria y Competitividad, and CERCA Program/Generalitat de Catalunya to A.J. Peer reviewed 2021-12-16T07:14:44Z 2021-12-16T07:14:44Z 2021-11-16 artículo http://purl.org/coar/resource_type/c_6501 Communications Biology 4(1): 1295 (2021) http://hdl.handle.net/10261/256154 10.1038/s42003-021-02820-9 2399-3642 http://dx.doi.org/10.13039/501100002809 http://dx.doi.org/10.13039/501100003329 34785760 2-s2.0-85119104837 https://api.elsevier.com/content/abstract/scopus_id/85119104837 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-76412-C2-1-R info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107479RB-I00 Communications biology Publisher's version https://doi.org/10.1038/s42003-021-02820-9 Sí open Springer Nature |
institution |
IATA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-iata-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del IATA España |
language |
English |
topic |
Microbial genetics EAEC virulence AggR expression Microbial genetics EAEC virulence AggR expression |
spellingShingle |
Microbial genetics EAEC virulence AggR expression Microbial genetics EAEC virulence AggR expression Prieto, Alejandro Bernabeu, Manuel Sánchez Herrero, José Francisco Pérez Bosque, Anna Miró, Lluïsa Bäuerl, Christine Collado, María Carmen Hüttener, Mário Juárez, Antonio Modulation of AggR levels reveals features of virulence regulation in enteroaggregative E. coli |
description |
Enteroaggregative Escherichia coli (EAEC) strains are one of the diarrheagenic pathotypes. EAEC strains harbor a virulence plasmid (pAA2) that encodes, among other virulence determinants, the aggR gene. The expression of the AggR protein leads to the expression of several virulence determinants in both plasmids and chromosomes. In this work, we describe a novel mechanism that influences AggR expression. Because of the absence of a Rho-independent terminator in the 3'UTR, aggR transcripts extend far beyond the aggR ORF. These transcripts are prone to PNPase-mediated degradation. Structural alterations in the 3'UTR result in increased aggR transcript stability, leading to increased AggR levels. We therefore investigated the effect of increased AggR levels on EAEC virulence. Upon finding the previously described AggR-dependent virulence factors, we detected novel AggR-regulated genes that may play relevant roles in EAEC virulence. Mutants exhibiting high AggR levels because of structural alterations in the aggR 3'UTR show increased mobility and increased pAA2 conjugation frequency. Furthermore, among the genes exhibiting increased fold change values, we could identify those of metabolic pathways that promote increased degradation of arginine, fatty acids and gamma-aminobutyric acid (GABA), respectively. In this paper, we discuss how the AggR-dependent increase in specific metabolic pathways activity may contribute to EAEC virulence. |
author2 |
Generalitat de Catalunya |
author_facet |
Generalitat de Catalunya Prieto, Alejandro Bernabeu, Manuel Sánchez Herrero, José Francisco Pérez Bosque, Anna Miró, Lluïsa Bäuerl, Christine Collado, María Carmen Hüttener, Mário Juárez, Antonio |
format |
artículo |
topic_facet |
Microbial genetics EAEC virulence AggR expression |
author |
Prieto, Alejandro Bernabeu, Manuel Sánchez Herrero, José Francisco Pérez Bosque, Anna Miró, Lluïsa Bäuerl, Christine Collado, María Carmen Hüttener, Mário Juárez, Antonio |
author_sort |
Prieto, Alejandro |
title |
Modulation of AggR levels reveals features of virulence regulation in enteroaggregative E. coli |
title_short |
Modulation of AggR levels reveals features of virulence regulation in enteroaggregative E. coli |
title_full |
Modulation of AggR levels reveals features of virulence regulation in enteroaggregative E. coli |
title_fullStr |
Modulation of AggR levels reveals features of virulence regulation in enteroaggregative E. coli |
title_full_unstemmed |
Modulation of AggR levels reveals features of virulence regulation in enteroaggregative E. coli |
title_sort |
modulation of aggr levels reveals features of virulence regulation in enteroaggregative e. coli |
publisher |
Springer Nature |
publishDate |
2021-11-16 |
url |
http://hdl.handle.net/10261/256154 http://dx.doi.org/10.13039/501100002809 http://dx.doi.org/10.13039/501100003329 https://api.elsevier.com/content/abstract/scopus_id/85119104837 |
work_keys_str_mv |
AT prietoalejandro modulationofaggrlevelsrevealsfeaturesofvirulenceregulationinenteroaggregativeecoli AT bernabeumanuel modulationofaggrlevelsrevealsfeaturesofvirulenceregulationinenteroaggregativeecoli AT sanchezherrerojosefrancisco modulationofaggrlevelsrevealsfeaturesofvirulenceregulationinenteroaggregativeecoli AT perezbosqueanna modulationofaggrlevelsrevealsfeaturesofvirulenceregulationinenteroaggregativeecoli AT mirolluisa modulationofaggrlevelsrevealsfeaturesofvirulenceregulationinenteroaggregativeecoli AT bauerlchristine modulationofaggrlevelsrevealsfeaturesofvirulenceregulationinenteroaggregativeecoli AT colladomariacarmen modulationofaggrlevelsrevealsfeaturesofvirulenceregulationinenteroaggregativeecoli AT huttenermario modulationofaggrlevelsrevealsfeaturesofvirulenceregulationinenteroaggregativeecoli AT juarezantonio modulationofaggrlevelsrevealsfeaturesofvirulenceregulationinenteroaggregativeecoli |
_version_ |
1777670208034439168 |