Endophytes from African Rice (Oryza glaberrima L.) Efficiently Colonize Asian Rice (Oryza sativa L.) Stimulating the Activity of Its Antioxidant Enzymes and Increasing the Content of Nitrogen, Carbon, and Chlorophyll
Bacterial endophytes support the adaptation of host plants to harsh environments. In this study, culturable bacterial endophytes were isolated from the African rice Oryza glaberrima L., which is well-adapted to grow with poor external inputs in the tropical region of Mali. Among these, six N-fixer strains were used to inoculate O. glaberrima RAM133 and the Asian rice O. sativa L. cv. Baldo, selected for growth in temperate climates. The colonization efficiency and the N-fixing activity were evaluated and compared for the two rice varieties. Oryza sativa-inoculated plants showed a fairly good colonization efficiency and nitrogenase activity. The inoculation of Oryza sativa with the strains Klebsiella pasteurii BDA134-6 and Phytobacter diazotrophicus BDA59-3 led to the highest nitrogenase activity. In addition, the inoculation of ‘Baldo’ plants with the strain P. diazotrophicus BDA59-3 led to a significant increase in nitrogen, carbon and chlorophyll content. Finally, ‘Baldo’ plants inoculated with Kl. pasteurii BDA134-6 showed the induction of antioxidant enzymes activity and the maintenance of nitrogen-fixation under salt stress as compared to the unstressed controls. As these endophytes efficiently colonize high-yielding crop varieties grown in cold temperate climates, they become good candidates to promote their growth under unfavorable conditions.
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Multidisciplinary Digital Publishing Institute
2021-08-11
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Subjects: | Nitrogen fixation, Indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, Rice endophytic bacteria, Salt stress, Anti-oxidative enzymes, |
Online Access: | http://hdl.handle.net/10261/267888 http://dx.doi.org/10.13039/501100003407 http://dx.doi.org/10.13039/501100005401 http://dx.doi.org/10.13039/501100004434 |
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dig-ias-es-10261-2678882022-04-28T01:46:43Z Endophytes from African Rice (Oryza glaberrima L.) Efficiently Colonize Asian Rice (Oryza sativa L.) Stimulating the Activity of Its Antioxidant Enzymes and Increasing the Content of Nitrogen, Carbon, and Chlorophyll Bianco, Carmen Andreozzi, Anna Romano, Silvia Fagorzi, Camilla Cangioli, Lisa Perini, Matteo Mengoni, Alessio Defez, Roberto Ministero delle Politiche Agricole Alimentari e Forestali Università degli Studi di Firenze Ministero dell'Istruzione, dell'Università e della Ricerca Nitrogen fixation Indole-3-acetic acid (IAA) 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase Rice endophytic bacteria Salt stress Anti-oxidative enzymes Bacterial endophytes support the adaptation of host plants to harsh environments. In this study, culturable bacterial endophytes were isolated from the African rice Oryza glaberrima L., which is well-adapted to grow with poor external inputs in the tropical region of Mali. Among these, six N-fixer strains were used to inoculate O. glaberrima RAM133 and the Asian rice O. sativa L. cv. Baldo, selected for growth in temperate climates. The colonization efficiency and the N-fixing activity were evaluated and compared for the two rice varieties. Oryza sativa-inoculated plants showed a fairly good colonization efficiency and nitrogenase activity. The inoculation of Oryza sativa with the strains Klebsiella pasteurii BDA134-6 and Phytobacter diazotrophicus BDA59-3 led to the highest nitrogenase activity. In addition, the inoculation of ‘Baldo’ plants with the strain P. diazotrophicus BDA59-3 led to a significant increase in nitrogen, carbon and chlorophyll content. Finally, ‘Baldo’ plants inoculated with Kl. pasteurii BDA134-6 showed the induction of antioxidant enzymes activity and the maintenance of nitrogen-fixation under salt stress as compared to the unstressed controls. As these endophytes efficiently colonize high-yielding crop varieties grown in cold temperate climates, they become good candidates to promote their growth under unfavorable conditions. This work was supported by the grant MICRO4Legumes (“Il microbioma vegetale simbionte come strumento per il miglioramento delle leguminose foraggere”), D.M.n.89267 (Italian Ministry of Agriculture). Genome sequencing of strains was partially supported by the grant as “Dipartimento di Eccellenza 2018-2022” awarded to the Dipartimento di Biologia, Università di Firenze, by the Italian Ministry of Education, University and Research (MIUR). 2022-04-27T07:20:03Z 2022-04-27T07:20:03Z 2021-08-11 2022-04-27T07:20:04Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.3390/microorganisms9081714 e-issn: 2076-2607 Microorganisms 9(8): 1714 (2021) http://hdl.handle.net/10261/267888 10.3390/microorganisms9081714 http://dx.doi.org/10.13039/501100003407 http://dx.doi.org/10.13039/501100005401 http://dx.doi.org/10.13039/501100004434 Publisher's version http://dx.doi.org/10.3390/microorganisms9081714 Sí open Multidisciplinary Digital Publishing Institute |
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Nitrogen fixation Indole-3-acetic acid (IAA) 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase Rice endophytic bacteria Salt stress Anti-oxidative enzymes Nitrogen fixation Indole-3-acetic acid (IAA) 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase Rice endophytic bacteria Salt stress Anti-oxidative enzymes |
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Nitrogen fixation Indole-3-acetic acid (IAA) 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase Rice endophytic bacteria Salt stress Anti-oxidative enzymes Nitrogen fixation Indole-3-acetic acid (IAA) 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase Rice endophytic bacteria Salt stress Anti-oxidative enzymes Bianco, Carmen Andreozzi, Anna Romano, Silvia Fagorzi, Camilla Cangioli, Lisa Perini, Matteo Mengoni, Alessio Defez, Roberto Endophytes from African Rice (Oryza glaberrima L.) Efficiently Colonize Asian Rice (Oryza sativa L.) Stimulating the Activity of Its Antioxidant Enzymes and Increasing the Content of Nitrogen, Carbon, and Chlorophyll |
description |
Bacterial endophytes support the adaptation of host plants to harsh environments. In this study, culturable bacterial endophytes were isolated from the African rice Oryza glaberrima L., which is well-adapted to grow with poor external inputs in the tropical region of Mali. Among these, six N-fixer strains were used to inoculate O. glaberrima RAM133 and the Asian rice O. sativa L. cv. Baldo, selected for growth in temperate climates. The colonization efficiency and the N-fixing activity were evaluated and compared for the two rice varieties. Oryza sativa-inoculated plants showed a fairly good colonization efficiency and nitrogenase activity. The inoculation of Oryza sativa with the strains Klebsiella pasteurii BDA134-6 and Phytobacter diazotrophicus BDA59-3 led to the highest nitrogenase activity. In addition, the inoculation of ‘Baldo’ plants with the strain P. diazotrophicus BDA59-3 led to a significant increase in nitrogen, carbon and chlorophyll content. Finally, ‘Baldo’ plants inoculated with Kl. pasteurii BDA134-6 showed the induction of antioxidant enzymes activity and the maintenance of nitrogen-fixation under salt stress as compared to the unstressed controls. As these endophytes efficiently colonize high-yielding crop varieties grown in cold temperate climates, they become good candidates to promote their growth under unfavorable conditions. |
author2 |
Ministero delle Politiche Agricole Alimentari e Forestali |
author_facet |
Ministero delle Politiche Agricole Alimentari e Forestali Bianco, Carmen Andreozzi, Anna Romano, Silvia Fagorzi, Camilla Cangioli, Lisa Perini, Matteo Mengoni, Alessio Defez, Roberto |
format |
artículo |
topic_facet |
Nitrogen fixation Indole-3-acetic acid (IAA) 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase Rice endophytic bacteria Salt stress Anti-oxidative enzymes |
author |
Bianco, Carmen Andreozzi, Anna Romano, Silvia Fagorzi, Camilla Cangioli, Lisa Perini, Matteo Mengoni, Alessio Defez, Roberto |
author_sort |
Bianco, Carmen |
title |
Endophytes from African Rice (Oryza glaberrima L.) Efficiently Colonize Asian Rice (Oryza sativa L.) Stimulating the Activity of Its Antioxidant Enzymes and Increasing the Content of Nitrogen, Carbon, and Chlorophyll |
title_short |
Endophytes from African Rice (Oryza glaberrima L.) Efficiently Colonize Asian Rice (Oryza sativa L.) Stimulating the Activity of Its Antioxidant Enzymes and Increasing the Content of Nitrogen, Carbon, and Chlorophyll |
title_full |
Endophytes from African Rice (Oryza glaberrima L.) Efficiently Colonize Asian Rice (Oryza sativa L.) Stimulating the Activity of Its Antioxidant Enzymes and Increasing the Content of Nitrogen, Carbon, and Chlorophyll |
title_fullStr |
Endophytes from African Rice (Oryza glaberrima L.) Efficiently Colonize Asian Rice (Oryza sativa L.) Stimulating the Activity of Its Antioxidant Enzymes and Increasing the Content of Nitrogen, Carbon, and Chlorophyll |
title_full_unstemmed |
Endophytes from African Rice (Oryza glaberrima L.) Efficiently Colonize Asian Rice (Oryza sativa L.) Stimulating the Activity of Its Antioxidant Enzymes and Increasing the Content of Nitrogen, Carbon, and Chlorophyll |
title_sort |
endophytes from african rice (oryza glaberrima l.) efficiently colonize asian rice (oryza sativa l.) stimulating the activity of its antioxidant enzymes and increasing the content of nitrogen, carbon, and chlorophyll |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021-08-11 |
url |
http://hdl.handle.net/10261/267888 http://dx.doi.org/10.13039/501100003407 http://dx.doi.org/10.13039/501100005401 http://dx.doi.org/10.13039/501100004434 |
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