Verticillium dahliae does not significantly change the structure and function of the olive belowground microbial communities but alters their co-occurrence interactions

Verticillium dahliae does not significantly change the structure and function of the olive belowground microbial communities but alters their co-occurrence interactions

Trabajo presentado en el Congress of Microbiology and Biotechnology (Microbiotec 19), celebrado en Coimbra del 5 al 7 de diciembre de 2019.

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Main Authors: Cardoni, Martina, Fernández-González, Antonio José, Gómez-Lama Cabanás, Carmen, Valverde-Corredor, Antonio, Villadas, Pablo J., Fernández-López, Manuel, Mercado-Blanco, Jesús
Format: comunicación de congreso biblioteca
Language:English
Published: 2019
Online Access:http://hdl.handle.net/10261/208436
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spelling dig-ias-es-10261-2084362020-04-22T01:09:42Z Verticillium dahliae does not significantly change the structure and function of the olive belowground microbial communities but alters their co-occurrence interactions Cardoni, Martina Fernández-González, Antonio José Gómez-Lama Cabanás, Carmen Valverde-Corredor, Antonio Villadas, Pablo J. Fernández-López, Manuel Mercado-Blanco, Jesús Trabajo presentado en el Congress of Microbiology and Biotechnology (Microbiotec 19), celebrado en Coimbra del 5 al 7 de diciembre de 2019. Verticillium wilt of olive (VWO), caused by the soilborne fungus Verticillium dahliae Kleb, is a relevant disease affecting olive cultivation in many areas where this tree is cultivated. The use of tolerant/resistant cultivars is considered an efficient control tool of the disease. A greenhouse experiment was set up to: (i) describe the root-associated microbial communities of two olive cultivars (cv.) qualified as tolerant (cv. Frantoio) and susceptible (cv. Picual) to V. dahliae, and (ii) assess whether the belowground-associated microbiome participate in the tolerance/susceptibility level of these cultivars to VWO. Nursery-produced olive plants were grown in pots containing non-sterile ad hoc prepared soil. After acclimation, a group of plants were inoculated with a defoliating (highly-virulent) representative of V. dahliae. A control group of non-inoculated plants was treated just with water. Surface disinfected roots and rhizosphere soil from both groups of plans were sampled at 0, 8, 15, and 30 (four plants per time-point and per cultivar) days after inoculation. DNA and RNA were extracted and sequenced (PCR products of the V3-V4 region of the 16S rRNA gene for bacteria and the ITS2 for fungi) by Illumina MiSeq. Bioinformatic, statistical and network analyses were then performed to analyze the composition, functionality and co-occurrence interactions of the endosphere and rhizosphere microbial communities. The belowground microbial communities of the two cultivars are similar, minor significant differences in diversity and composition of root-associated microbiota were found between olive cultivars regardless they were inoculated or not with the pathogen. Presence of V. dahliaecaused changes mainly in communities’ taxa relative abundance, mostly in the VWO-susceptible cultivar. However, notable differences were found in the communities co-occurrence interactions in response to the pathogen presence. Both cultivars showed changes in the topology of the networks, both at DNA and RNA level, and modifications of the positive/negative edges ratio. A correlation between microbial networks modifications and susceptibility/tolerance to the pathogen was found. Therefore, changes in the microbial communities interactions may explain, at least partially, the differential VWO susceptibility of the tested olive cultivars. Peer reviewed 2020-04-21T11:06:54Z 2020-04-21T11:06:54Z 2019 comunicación de congreso http://purl.org/coar/resource_type/c_5794 Congress of Microbiology and Biotechnology (2019) http://hdl.handle.net/10261/208436 en Sí open
institution IAS ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-ias-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IAS España
language English
description Trabajo presentado en el Congress of Microbiology and Biotechnology (Microbiotec 19), celebrado en Coimbra del 5 al 7 de diciembre de 2019.
format comunicación de congreso
author Cardoni, Martina
Fernández-González, Antonio José
Gómez-Lama Cabanás, Carmen
Valverde-Corredor, Antonio
Villadas, Pablo J.
Fernández-López, Manuel
Mercado-Blanco, Jesús
spellingShingle Cardoni, Martina
Fernández-González, Antonio José
Gómez-Lama Cabanás, Carmen
Valverde-Corredor, Antonio
Villadas, Pablo J.
Fernández-López, Manuel
Mercado-Blanco, Jesús
Verticillium dahliae does not significantly change the structure and function of the olive belowground microbial communities but alters their co-occurrence interactions
author_facet Cardoni, Martina
Fernández-González, Antonio José
Gómez-Lama Cabanás, Carmen
Valverde-Corredor, Antonio
Villadas, Pablo J.
Fernández-López, Manuel
Mercado-Blanco, Jesús
author_sort Cardoni, Martina
title Verticillium dahliae does not significantly change the structure and function of the olive belowground microbial communities but alters their co-occurrence interactions
title_short Verticillium dahliae does not significantly change the structure and function of the olive belowground microbial communities but alters their co-occurrence interactions
title_full Verticillium dahliae does not significantly change the structure and function of the olive belowground microbial communities but alters their co-occurrence interactions
title_fullStr Verticillium dahliae does not significantly change the structure and function of the olive belowground microbial communities but alters their co-occurrence interactions
title_full_unstemmed Verticillium dahliae does not significantly change the structure and function of the olive belowground microbial communities but alters their co-occurrence interactions
title_sort verticillium dahliae does not significantly change the structure and function of the olive belowground microbial communities but alters their co-occurrence interactions
publishDate 2019
url http://hdl.handle.net/10261/208436
work_keys_str_mv AT cardonimartina verticilliumdahliaedoesnotsignificantlychangethestructureandfunctionoftheolivebelowgroundmicrobialcommunitiesbutalterstheircooccurrenceinteractions
AT fernandezgonzalezantoniojose verticilliumdahliaedoesnotsignificantlychangethestructureandfunctionoftheolivebelowgroundmicrobialcommunitiesbutalterstheircooccurrenceinteractions
AT gomezlamacabanascarmen verticilliumdahliaedoesnotsignificantlychangethestructureandfunctionoftheolivebelowgroundmicrobialcommunitiesbutalterstheircooccurrenceinteractions
AT valverdecorredorantonio verticilliumdahliaedoesnotsignificantlychangethestructureandfunctionoftheolivebelowgroundmicrobialcommunitiesbutalterstheircooccurrenceinteractions
AT villadaspabloj verticilliumdahliaedoesnotsignificantlychangethestructureandfunctionoftheolivebelowgroundmicrobialcommunitiesbutalterstheircooccurrenceinteractions
AT fernandezlopezmanuel verticilliumdahliaedoesnotsignificantlychangethestructureandfunctionoftheolivebelowgroundmicrobialcommunitiesbutalterstheircooccurrenceinteractions
AT mercadoblancojesus verticilliumdahliaedoesnotsignificantlychangethestructureandfunctionoftheolivebelowgroundmicrobialcommunitiesbutalterstheircooccurrenceinteractions
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