Overexpression of EcGSH1 induces glutathione production and alters somatic embryogenesis and plant development in Hevea brasiliensis
Oxidative stress occurring during in-vitro culture is detrimental for clonal propagation by somatic embryogenesis in particular in Hevea brasiliensis. In previous work, overexpression of the gene encoding the cytosolic reactive oxygen species detoxification enzyme HbCuZnSOD led to reduce somatic embryo regeneration in Hevea. In this study, the role of antioxidant was tested by overexpression of the E.coli GSH1 gene involved in glutathione biosynthesis in rubber embryogenic callus lines. Transgenic lines were successfully established and some plants were regenerated. Overexpression of EcGSH1 gene led to glutathione over-accumulation, and affected dramatically the somatic embryogenesis process and plant development. Upon a water deficit treatment, these plants displayed the greatest drop in photosynthetic nitrogen use efficiency, higher proline content, and higher glutathione reductase activity. Changes induced in transgenic lines overexpressing HbCuZnSOD and EcGSH1 were discussed as well as possible applications on plant material propagation, overcoming loss of natural rubber production through Tapping Panel Dryness, and tropical soil remediation.
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Subjects: | F30 - Génétique et amélioration des plantes, F62 - Physiologie végétale - Croissance et développement, Hevea brasiliensis, Embryogénèse somatique, culture in vitro, micropropagation, clone, expression des gènes, glutathion, biosynthèse, caoutchouc, http://aims.fao.org/aos/agrovoc/c_3589, http://aims.fao.org/aos/agrovoc/c_36911, http://aims.fao.org/aos/agrovoc/c_24135, http://aims.fao.org/aos/agrovoc/c_24136, http://aims.fao.org/aos/agrovoc/c_1678, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_11184, http://aims.fao.org/aos/agrovoc/c_928, http://aims.fao.org/aos/agrovoc/c_6678, |
Online Access: | http://agritrop.cirad.fr/587064/ http://agritrop.cirad.fr/587064/1/1-s2.0-S0926669017308890-main.pdf |
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dig-cirad-fr-5870642024-01-29T00:49:57Z http://agritrop.cirad.fr/587064/ http://agritrop.cirad.fr/587064/ Overexpression of EcGSH1 induces glutathione production and alters somatic embryogenesis and plant development in Hevea brasiliensis. Martin Florence, Abati Vanessa, Burel Arnaud, Clément-Vidal Anne, Sanier Christine, Fabre Denis, Woraathasin Natthakorn, Rio Maryannick, Besret Pierre, Farinas Benoît, Montoro Pascal, Leclercq Julie. 2018. Industrial Crops and Products, 112 : 803-814.https://doi.org/10.1016/j.indcrop.2017.12.057 <https://doi.org/10.1016/j.indcrop.2017.12.057> Overexpression of EcGSH1 induces glutathione production and alters somatic embryogenesis and plant development in Hevea brasiliensis Martin, Florence Abati, Vanessa Burel, Arnaud Clément-Vidal, Anne Sanier, Christine Fabre, Denis Woraathasin, Natthakorn Rio, Maryannick Besret, Pierre Farinas, Benoît Montoro, Pascal Leclercq, Julie eng 2018 Industrial Crops and Products F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Hevea brasiliensis Embryogénèse somatique culture in vitro micropropagation clone expression des gènes glutathion biosynthèse caoutchouc http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_24136 http://aims.fao.org/aos/agrovoc/c_1678 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_11184 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_6678 Oxidative stress occurring during in-vitro culture is detrimental for clonal propagation by somatic embryogenesis in particular in Hevea brasiliensis. In previous work, overexpression of the gene encoding the cytosolic reactive oxygen species detoxification enzyme HbCuZnSOD led to reduce somatic embryo regeneration in Hevea. In this study, the role of antioxidant was tested by overexpression of the E.coli GSH1 gene involved in glutathione biosynthesis in rubber embryogenic callus lines. Transgenic lines were successfully established and some plants were regenerated. Overexpression of EcGSH1 gene led to glutathione over-accumulation, and affected dramatically the somatic embryogenesis process and plant development. Upon a water deficit treatment, these plants displayed the greatest drop in photosynthetic nitrogen use efficiency, higher proline content, and higher glutathione reductase activity. Changes induced in transgenic lines overexpressing HbCuZnSOD and EcGSH1 were discussed as well as possible applications on plant material propagation, overcoming loss of natural rubber production through Tapping Panel Dryness, and tropical soil remediation. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/587064/1/1-s2.0-S0926669017308890-main.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.indcrop.2017.12.057 10.1016/j.indcrop.2017.12.057 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.indcrop.2017.12.057 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.indcrop.2017.12.057 |
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F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Hevea brasiliensis Embryogénèse somatique culture in vitro micropropagation clone expression des gènes glutathion biosynthèse caoutchouc http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_24136 http://aims.fao.org/aos/agrovoc/c_1678 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_11184 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_6678 F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Hevea brasiliensis Embryogénèse somatique culture in vitro micropropagation clone expression des gènes glutathion biosynthèse caoutchouc http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_24136 http://aims.fao.org/aos/agrovoc/c_1678 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_11184 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_6678 |
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F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Hevea brasiliensis Embryogénèse somatique culture in vitro micropropagation clone expression des gènes glutathion biosynthèse caoutchouc http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_24136 http://aims.fao.org/aos/agrovoc/c_1678 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_11184 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_6678 F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Hevea brasiliensis Embryogénèse somatique culture in vitro micropropagation clone expression des gènes glutathion biosynthèse caoutchouc http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_24136 http://aims.fao.org/aos/agrovoc/c_1678 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_11184 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_6678 Martin, Florence Abati, Vanessa Burel, Arnaud Clément-Vidal, Anne Sanier, Christine Fabre, Denis Woraathasin, Natthakorn Rio, Maryannick Besret, Pierre Farinas, Benoît Montoro, Pascal Leclercq, Julie Overexpression of EcGSH1 induces glutathione production and alters somatic embryogenesis and plant development in Hevea brasiliensis |
description |
Oxidative stress occurring during in-vitro culture is detrimental for clonal propagation by somatic embryogenesis in particular in Hevea brasiliensis. In previous work, overexpression of the gene encoding the cytosolic reactive oxygen species detoxification enzyme HbCuZnSOD led to reduce somatic embryo regeneration in Hevea. In this study, the role of antioxidant was tested by overexpression of the E.coli GSH1 gene involved in glutathione biosynthesis in rubber embryogenic callus lines. Transgenic lines were successfully established and some plants were regenerated. Overexpression of EcGSH1 gene led to glutathione over-accumulation, and affected dramatically the somatic embryogenesis process and plant development. Upon a water deficit treatment, these plants displayed the greatest drop in photosynthetic nitrogen use efficiency, higher proline content, and higher glutathione reductase activity. Changes induced in transgenic lines overexpressing HbCuZnSOD and EcGSH1 were discussed as well as possible applications on plant material propagation, overcoming loss of natural rubber production through Tapping Panel Dryness, and tropical soil remediation. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Hevea brasiliensis Embryogénèse somatique culture in vitro micropropagation clone expression des gènes glutathion biosynthèse caoutchouc http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_24136 http://aims.fao.org/aos/agrovoc/c_1678 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_11184 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_6678 |
author |
Martin, Florence Abati, Vanessa Burel, Arnaud Clément-Vidal, Anne Sanier, Christine Fabre, Denis Woraathasin, Natthakorn Rio, Maryannick Besret, Pierre Farinas, Benoît Montoro, Pascal Leclercq, Julie |
author_facet |
Martin, Florence Abati, Vanessa Burel, Arnaud Clément-Vidal, Anne Sanier, Christine Fabre, Denis Woraathasin, Natthakorn Rio, Maryannick Besret, Pierre Farinas, Benoît Montoro, Pascal Leclercq, Julie |
author_sort |
Martin, Florence |
title |
Overexpression of EcGSH1 induces glutathione production and alters somatic embryogenesis and plant development in Hevea brasiliensis |
title_short |
Overexpression of EcGSH1 induces glutathione production and alters somatic embryogenesis and plant development in Hevea brasiliensis |
title_full |
Overexpression of EcGSH1 induces glutathione production and alters somatic embryogenesis and plant development in Hevea brasiliensis |
title_fullStr |
Overexpression of EcGSH1 induces glutathione production and alters somatic embryogenesis and plant development in Hevea brasiliensis |
title_full_unstemmed |
Overexpression of EcGSH1 induces glutathione production and alters somatic embryogenesis and plant development in Hevea brasiliensis |
title_sort |
overexpression of ecgsh1 induces glutathione production and alters somatic embryogenesis and plant development in hevea brasiliensis |
url |
http://agritrop.cirad.fr/587064/ http://agritrop.cirad.fr/587064/1/1-s2.0-S0926669017308890-main.pdf |
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