A new suite of twenty-two polymorphic microsatellite loci in the parasitic wasp, Habrobracon hebetor (Hymenoptera: Braconidae): Promising molecular tools for studying the population genetics of several beneficial braconid species
Combining a biotin-enrichment protocol and 454GS-FLX titanium pyrosequencing technology, we characterised 22 polymorphic microsatellite loci from the parasitic wasp, Habrobracon hebetor (Say) (Hymenoptera: Braconidae), a cosmopolitan species commonly used in biological control against a wide range of both major lepidopterous pests of stored products and field crops in different parts of the world. Three multiplex PCR sets were optimised and characterised across 46 H. hebetor specimens from two samples collected from millet fields in Niger. Two to 11 alleles were found per locus and observed heterozygosity ranged from 0.289 to 0.826. Polymorphism was detected in both samples with a similar level of observed heterozygosity (0.482 vs. 0.502) and number of alleles (4.1 vs. 3.6). Deviation from Hardy-Weinberg equilibrium was detected at the same five loci in both samples and five or seven more loci in each sample but was not associated with heterozygote deficiencies. Even though evidence for linkage disequilibrium was found between a few alleles, these new loci segregated independently. The variability of the 22 loci will enable estimates of genetic diversity and structure patterns, as well as gene flow between H. hebetor populations at different spatial scales. Cross-species amplifications were successful among the six Bracon spp. tested and nine loci will be particularly appropriate for population genetic studies in B. brevicornis.
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Subjects: | H10 - Ravageurs des plantes, J11 - Manutention, transport, stockage et conservation des produits d'origine végétale, polymorphisme génétique, lutte antiparasite en entreposage, locus, microsatellite, variation génétique, http://aims.fao.org/aos/agrovoc/c_24031, http://aims.fao.org/aos/agrovoc/c_7432, http://aims.fao.org/aos/agrovoc/c_24869, http://aims.fao.org/aos/agrovoc/c_36574, http://aims.fao.org/aos/agrovoc/c_15975, |
Online Access: | http://agritrop.cirad.fr/582337/ http://agritrop.cirad.fr/582337/1/Benoit-European%20Journal%20of%20Entomology-2016-113%20pp265-269.pdf |
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dig-cirad-fr-5823372024-01-29T05:36:51Z http://agritrop.cirad.fr/582337/ http://agritrop.cirad.fr/582337/ A new suite of twenty-two polymorphic microsatellite loci in the parasitic wasp, Habrobracon hebetor (Hymenoptera: Braconidae): Promising molecular tools for studying the population genetics of several beneficial braconid species. Garba Madougou, Loiseau Anne, Benoit Laure, Gauthier Nathalie. 2016. European Journal of Entomology, 113 : 265-269.https://doi.org/10.14411/eje.2016.031 <https://doi.org/10.14411/eje.2016.031> A new suite of twenty-two polymorphic microsatellite loci in the parasitic wasp, Habrobracon hebetor (Hymenoptera: Braconidae): Promising molecular tools for studying the population genetics of several beneficial braconid species Garba, Madougou Loiseau, Anne Benoit, Laure Gauthier, Nathalie eng 2016 European Journal of Entomology H10 - Ravageurs des plantes J11 - Manutention, transport, stockage et conservation des produits d'origine végétale polymorphisme génétique lutte antiparasite en entreposage locus microsatellite variation génétique http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_7432 http://aims.fao.org/aos/agrovoc/c_24869 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_15975 Combining a biotin-enrichment protocol and 454GS-FLX titanium pyrosequencing technology, we characterised 22 polymorphic microsatellite loci from the parasitic wasp, Habrobracon hebetor (Say) (Hymenoptera: Braconidae), a cosmopolitan species commonly used in biological control against a wide range of both major lepidopterous pests of stored products and field crops in different parts of the world. Three multiplex PCR sets were optimised and characterised across 46 H. hebetor specimens from two samples collected from millet fields in Niger. Two to 11 alleles were found per locus and observed heterozygosity ranged from 0.289 to 0.826. Polymorphism was detected in both samples with a similar level of observed heterozygosity (0.482 vs. 0.502) and number of alleles (4.1 vs. 3.6). Deviation from Hardy-Weinberg equilibrium was detected at the same five loci in both samples and five or seven more loci in each sample but was not associated with heterozygote deficiencies. Even though evidence for linkage disequilibrium was found between a few alleles, these new loci segregated independently. The variability of the 22 loci will enable estimates of genetic diversity and structure patterns, as well as gene flow between H. hebetor populations at different spatial scales. Cross-species amplifications were successful among the six Bracon spp. tested and nine loci will be particularly appropriate for population genetic studies in B. brevicornis. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/582337/1/Benoit-European%20Journal%20of%20Entomology-2016-113%20pp265-269.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.14411/eje.2016.031 10.14411/eje.2016.031 info:eu-repo/semantics/altIdentifier/doi/10.14411/eje.2016.031 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.14411/eje.2016.031 |
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H10 - Ravageurs des plantes J11 - Manutention, transport, stockage et conservation des produits d'origine végétale polymorphisme génétique lutte antiparasite en entreposage locus microsatellite variation génétique http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_7432 http://aims.fao.org/aos/agrovoc/c_24869 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_15975 H10 - Ravageurs des plantes J11 - Manutention, transport, stockage et conservation des produits d'origine végétale polymorphisme génétique lutte antiparasite en entreposage locus microsatellite variation génétique http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_7432 http://aims.fao.org/aos/agrovoc/c_24869 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_15975 |
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H10 - Ravageurs des plantes J11 - Manutention, transport, stockage et conservation des produits d'origine végétale polymorphisme génétique lutte antiparasite en entreposage locus microsatellite variation génétique http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_7432 http://aims.fao.org/aos/agrovoc/c_24869 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_15975 H10 - Ravageurs des plantes J11 - Manutention, transport, stockage et conservation des produits d'origine végétale polymorphisme génétique lutte antiparasite en entreposage locus microsatellite variation génétique http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_7432 http://aims.fao.org/aos/agrovoc/c_24869 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_15975 Garba, Madougou Loiseau, Anne Benoit, Laure Gauthier, Nathalie A new suite of twenty-two polymorphic microsatellite loci in the parasitic wasp, Habrobracon hebetor (Hymenoptera: Braconidae): Promising molecular tools for studying the population genetics of several beneficial braconid species |
description |
Combining a biotin-enrichment protocol and 454GS-FLX titanium pyrosequencing technology, we characterised 22 polymorphic microsatellite loci from the parasitic wasp, Habrobracon hebetor (Say) (Hymenoptera: Braconidae), a cosmopolitan species commonly used in biological control against a wide range of both major lepidopterous pests of stored products and field crops in different parts of the world. Three multiplex PCR sets were optimised and characterised across 46 H. hebetor specimens from two samples collected from millet fields in Niger. Two to 11 alleles were found per locus and observed heterozygosity ranged from 0.289 to 0.826. Polymorphism was detected in both samples with a similar level of observed heterozygosity (0.482 vs. 0.502) and number of alleles (4.1 vs. 3.6). Deviation from Hardy-Weinberg equilibrium was detected at the same five loci in both samples and five or seven more loci in each sample but was not associated with heterozygote deficiencies. Even though evidence for linkage disequilibrium was found between a few alleles, these new loci segregated independently. The variability of the 22 loci will enable estimates of genetic diversity and structure patterns, as well as gene flow between H. hebetor populations at different spatial scales. Cross-species amplifications were successful among the six Bracon spp. tested and nine loci will be particularly appropriate for population genetic studies in B. brevicornis. |
format |
article |
topic_facet |
H10 - Ravageurs des plantes J11 - Manutention, transport, stockage et conservation des produits d'origine végétale polymorphisme génétique lutte antiparasite en entreposage locus microsatellite variation génétique http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_7432 http://aims.fao.org/aos/agrovoc/c_24869 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_15975 |
author |
Garba, Madougou Loiseau, Anne Benoit, Laure Gauthier, Nathalie |
author_facet |
Garba, Madougou Loiseau, Anne Benoit, Laure Gauthier, Nathalie |
author_sort |
Garba, Madougou |
title |
A new suite of twenty-two polymorphic microsatellite loci in the parasitic wasp, Habrobracon hebetor (Hymenoptera: Braconidae): Promising molecular tools for studying the population genetics of several beneficial braconid species |
title_short |
A new suite of twenty-two polymorphic microsatellite loci in the parasitic wasp, Habrobracon hebetor (Hymenoptera: Braconidae): Promising molecular tools for studying the population genetics of several beneficial braconid species |
title_full |
A new suite of twenty-two polymorphic microsatellite loci in the parasitic wasp, Habrobracon hebetor (Hymenoptera: Braconidae): Promising molecular tools for studying the population genetics of several beneficial braconid species |
title_fullStr |
A new suite of twenty-two polymorphic microsatellite loci in the parasitic wasp, Habrobracon hebetor (Hymenoptera: Braconidae): Promising molecular tools for studying the population genetics of several beneficial braconid species |
title_full_unstemmed |
A new suite of twenty-two polymorphic microsatellite loci in the parasitic wasp, Habrobracon hebetor (Hymenoptera: Braconidae): Promising molecular tools for studying the population genetics of several beneficial braconid species |
title_sort |
new suite of twenty-two polymorphic microsatellite loci in the parasitic wasp, habrobracon hebetor (hymenoptera: braconidae): promising molecular tools for studying the population genetics of several beneficial braconid species |
url |
http://agritrop.cirad.fr/582337/ http://agritrop.cirad.fr/582337/1/Benoit-European%20Journal%20of%20Entomology-2016-113%20pp265-269.pdf |
work_keys_str_mv |
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