Comparison of bacterial communities of tilapia fish from Cameroon and Vietnam using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis)
Fishes in general and tilapia in particular are traded all over the world. However, it is difficult to find out their exact geographical location. One of the techniques used in the traceability of fish and its byproducts consist in analysing in a global way the whole viable and non viable bacterial communities. For this purpose, the molecular technique employing the bacterial 16S DNA banding profiles generated by PCR-DGGE (polymerase chain reaction-Denaturing gradient gel electrophoresis) was used to evaluate the differences between the bacterial profiles of fishes from Vietnam (An Giang, south province) and those of Cameroon (Yagoua, Maga, Lagdo). The different PCR-DGGE 16S rDNA banding profiles obtained were analysed and results showed that there were specific bands for each geographical location though some bands common to Cameroon and Vietnam were observed. This method could be used as a rapid analytical traceability tool for fish products and could be considered as a provider of a unique biological bar code.
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Format: | article biblioteca |
Language: | eng |
Subjects: | Q03 - Contamination et toxicologie alimentaires, M01 - Pêche et aquaculture - Considérations générales, Tilapia, flore microbienne, provenance, PCR, électrophorèse, http://aims.fao.org/aos/agrovoc/c_32720, http://aims.fao.org/aos/agrovoc/c_16367, http://aims.fao.org/aos/agrovoc/c_16022, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_2523, http://aims.fao.org/aos/agrovoc/c_1229, http://aims.fao.org/aos/agrovoc/c_8227, |
Online Access: | http://agritrop.cirad.fr/552840/ http://agritrop.cirad.fr/552840/1/document_552840.pdf |
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dig-cirad-fr-5528402024-01-28T17:55:33Z http://agritrop.cirad.fr/552840/ http://agritrop.cirad.fr/552840/ Comparison of bacterial communities of tilapia fish from Cameroon and Vietnam using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis). Maïworé Justine, Tatsadjieu Ngoune Léopold, Montet Didier, Loiseau Gérard, Mbofung C.M.F.. 2009. African Journal of Biotechnology, 8 (24) : 7156-7163. Comparison of bacterial communities of tilapia fish from Cameroon and Vietnam using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) Maïworé, Justine Tatsadjieu, Ngoune Léopold Montet, Didier Loiseau, Gérard Mbofung, C.M.F. eng 2009 African Journal of Biotechnology Q03 - Contamination et toxicologie alimentaires M01 - Pêche et aquaculture - Considérations générales Tilapia flore microbienne provenance PCR électrophorèse http://aims.fao.org/aos/agrovoc/c_32720 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_16022 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2523 Cameroun Viet Nam http://aims.fao.org/aos/agrovoc/c_1229 http://aims.fao.org/aos/agrovoc/c_8227 Fishes in general and tilapia in particular are traded all over the world. However, it is difficult to find out their exact geographical location. One of the techniques used in the traceability of fish and its byproducts consist in analysing in a global way the whole viable and non viable bacterial communities. For this purpose, the molecular technique employing the bacterial 16S DNA banding profiles generated by PCR-DGGE (polymerase chain reaction-Denaturing gradient gel electrophoresis) was used to evaluate the differences between the bacterial profiles of fishes from Vietnam (An Giang, south province) and those of Cameroon (Yagoua, Maga, Lagdo). The different PCR-DGGE 16S rDNA banding profiles obtained were analysed and results showed that there were specific bands for each geographical location though some bands common to Cameroon and Vietnam were observed. This method could be used as a rapid analytical traceability tool for fish products and could be considered as a provider of a unique biological bar code. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/552840/1/document_552840.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html |
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Q03 - Contamination et toxicologie alimentaires M01 - Pêche et aquaculture - Considérations générales Tilapia flore microbienne provenance PCR électrophorèse http://aims.fao.org/aos/agrovoc/c_32720 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_16022 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2523 http://aims.fao.org/aos/agrovoc/c_1229 http://aims.fao.org/aos/agrovoc/c_8227 Q03 - Contamination et toxicologie alimentaires M01 - Pêche et aquaculture - Considérations générales Tilapia flore microbienne provenance PCR électrophorèse http://aims.fao.org/aos/agrovoc/c_32720 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_16022 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2523 http://aims.fao.org/aos/agrovoc/c_1229 http://aims.fao.org/aos/agrovoc/c_8227 |
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Q03 - Contamination et toxicologie alimentaires M01 - Pêche et aquaculture - Considérations générales Tilapia flore microbienne provenance PCR électrophorèse http://aims.fao.org/aos/agrovoc/c_32720 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_16022 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2523 http://aims.fao.org/aos/agrovoc/c_1229 http://aims.fao.org/aos/agrovoc/c_8227 Q03 - Contamination et toxicologie alimentaires M01 - Pêche et aquaculture - Considérations générales Tilapia flore microbienne provenance PCR électrophorèse http://aims.fao.org/aos/agrovoc/c_32720 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_16022 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2523 http://aims.fao.org/aos/agrovoc/c_1229 http://aims.fao.org/aos/agrovoc/c_8227 Maïworé, Justine Tatsadjieu, Ngoune Léopold Montet, Didier Loiseau, Gérard Mbofung, C.M.F. Comparison of bacterial communities of tilapia fish from Cameroon and Vietnam using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) |
description |
Fishes in general and tilapia in particular are traded all over the world. However, it is difficult to find out their exact geographical location. One of the techniques used in the traceability of fish and its byproducts consist in analysing in a global way the whole viable and non viable bacterial communities. For this purpose, the molecular technique employing the bacterial 16S DNA banding profiles generated by PCR-DGGE (polymerase chain reaction-Denaturing gradient gel electrophoresis) was used to evaluate the differences between the bacterial profiles of fishes from Vietnam (An Giang, south province) and those of Cameroon (Yagoua, Maga, Lagdo). The different PCR-DGGE 16S rDNA banding profiles obtained were analysed and results showed that there were specific bands for each geographical location though some bands common to Cameroon and Vietnam were observed. This method could be used as a rapid analytical traceability tool for fish products and could be considered as a provider of a unique biological bar code. |
format |
article |
topic_facet |
Q03 - Contamination et toxicologie alimentaires M01 - Pêche et aquaculture - Considérations générales Tilapia flore microbienne provenance PCR électrophorèse http://aims.fao.org/aos/agrovoc/c_32720 http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_16022 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2523 http://aims.fao.org/aos/agrovoc/c_1229 http://aims.fao.org/aos/agrovoc/c_8227 |
author |
Maïworé, Justine Tatsadjieu, Ngoune Léopold Montet, Didier Loiseau, Gérard Mbofung, C.M.F. |
author_facet |
Maïworé, Justine Tatsadjieu, Ngoune Léopold Montet, Didier Loiseau, Gérard Mbofung, C.M.F. |
author_sort |
Maïworé, Justine |
title |
Comparison of bacterial communities of tilapia fish from Cameroon and Vietnam using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) |
title_short |
Comparison of bacterial communities of tilapia fish from Cameroon and Vietnam using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) |
title_full |
Comparison of bacterial communities of tilapia fish from Cameroon and Vietnam using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) |
title_fullStr |
Comparison of bacterial communities of tilapia fish from Cameroon and Vietnam using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) |
title_full_unstemmed |
Comparison of bacterial communities of tilapia fish from Cameroon and Vietnam using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) |
title_sort |
comparison of bacterial communities of tilapia fish from cameroon and vietnam using pcr-dgge (polymerase chain reaction-denaturing gradient gel electrophoresis) |
url |
http://agritrop.cirad.fr/552840/ http://agritrop.cirad.fr/552840/1/document_552840.pdf |
work_keys_str_mv |
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_version_ |
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