Species identification, molecular sexing and genotyping using non-invasive approaches in two wild bovids species: Bos gaurus and Bos javanicus
Since the second Indochina war, habitat destruction and overhunting has resulted in fragmentation of the remaining populations of Bos javanicus and B. gaurus. Nowadays, both species are in serious danger, especially the gaur. In Vietnam, where these species have become almost impossible to capture in the wild, noninvasive investigations are the only feasible approach to obtain data on populations. However, non-invasive derived DNA, especially in tropical areas, is usually characterized by low concentrations, poor quality and/or contamination from alien DNA. To assist in tropical conservation management, baseline information is provided here on assessing the reliability of species identification, molecular sexing and microsatellite genotyping using fecal DNA from B. gaurus and B. javanicus. For species identification using bovine fecal samples, cytochrome b fragment between positions 867 and 1140 was found to contain species diagnostic sites, which distinguishes the four species encountered in the region: B. gaurus, B. indicus, B. javanicus and B. taurus. For sex determination, primers were initially tested on DNA obtained from blood. Then, these primers were successfully used on DNA derived from fecal material. Finally, we also evaluate the feasibility of non-invasive microsatellite genotyping on fecal samples collected in Vietnamese nature reserves. The results presented here improve on current molecular methods based on fecal material obtained from tropical areas.
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Subjects: | U30 - Méthodes de recherche, F30 - Génétique et amélioration des plantes, L20 - Écologie animale, L60 - Taxonomie et géographie animales, gaur, Banteng, animal sauvage, http://aims.fao.org/aos/agrovoc/c_3205, http://aims.fao.org/aos/agrovoc/c_26758, http://aims.fao.org/aos/agrovoc/c_24103, http://aims.fao.org/aos/agrovoc/c_3081, http://aims.fao.org/aos/agrovoc/c_8227, |
Online Access: | http://agritrop.cirad.fr/548673/ http://agritrop.cirad.fr/548673/1/document_548673.pdf |
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dig-cirad-fr-5486732024-01-28T16:55:21Z http://agritrop.cirad.fr/548673/ http://agritrop.cirad.fr/548673/ Species identification, molecular sexing and genotyping using non-invasive approaches in two wild bovids species: Bos gaurus and Bos javanicus. Rivière-Dobigny Taiana, Pham Doan Lan, Le Quang Nam, Maillard Jean-Charles, Michaux Johan. 2009. Zoo Biology, 28 (2) : 127-136.https://doi.org/10.1002/zoo.20211 <https://doi.org/10.1002/zoo.20211> Species identification, molecular sexing and genotyping using non-invasive approaches in two wild bovids species: Bos gaurus and Bos javanicus Rivière-Dobigny, Taiana Pham Doan Lan, Le Quang Nam, Maillard, Jean-Charles Michaux, Johan eng 2009 Zoo Biology U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes L20 - Écologie animale L60 - Taxonomie et géographie animales gaur Banteng animal sauvage http://aims.fao.org/aos/agrovoc/c_3205 http://aims.fao.org/aos/agrovoc/c_26758 http://aims.fao.org/aos/agrovoc/c_24103 France Viet Nam http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_8227 Since the second Indochina war, habitat destruction and overhunting has resulted in fragmentation of the remaining populations of Bos javanicus and B. gaurus. Nowadays, both species are in serious danger, especially the gaur. In Vietnam, where these species have become almost impossible to capture in the wild, noninvasive investigations are the only feasible approach to obtain data on populations. However, non-invasive derived DNA, especially in tropical areas, is usually characterized by low concentrations, poor quality and/or contamination from alien DNA. To assist in tropical conservation management, baseline information is provided here on assessing the reliability of species identification, molecular sexing and microsatellite genotyping using fecal DNA from B. gaurus and B. javanicus. For species identification using bovine fecal samples, cytochrome b fragment between positions 867 and 1140 was found to contain species diagnostic sites, which distinguishes the four species encountered in the region: B. gaurus, B. indicus, B. javanicus and B. taurus. For sex determination, primers were initially tested on DNA obtained from blood. Then, these primers were successfully used on DNA derived from fecal material. Finally, we also evaluate the feasibility of non-invasive microsatellite genotyping on fecal samples collected in Vietnamese nature reserves. The results presented here improve on current molecular methods based on fecal material obtained from tropical areas. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/548673/1/document_548673.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1002/zoo.20211 10.1002/zoo.20211 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=30881 info:eu-repo/semantics/altIdentifier/doi/10.1002/zoo.20211 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1002/zoo.20211 |
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U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes L20 - Écologie animale L60 - Taxonomie et géographie animales gaur Banteng animal sauvage http://aims.fao.org/aos/agrovoc/c_3205 http://aims.fao.org/aos/agrovoc/c_26758 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_8227 U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes L20 - Écologie animale L60 - Taxonomie et géographie animales gaur Banteng animal sauvage http://aims.fao.org/aos/agrovoc/c_3205 http://aims.fao.org/aos/agrovoc/c_26758 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_8227 |
spellingShingle |
U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes L20 - Écologie animale L60 - Taxonomie et géographie animales gaur Banteng animal sauvage http://aims.fao.org/aos/agrovoc/c_3205 http://aims.fao.org/aos/agrovoc/c_26758 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_8227 U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes L20 - Écologie animale L60 - Taxonomie et géographie animales gaur Banteng animal sauvage http://aims.fao.org/aos/agrovoc/c_3205 http://aims.fao.org/aos/agrovoc/c_26758 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_8227 Rivière-Dobigny, Taiana Pham Doan Lan, Le Quang Nam, Maillard, Jean-Charles Michaux, Johan Species identification, molecular sexing and genotyping using non-invasive approaches in two wild bovids species: Bos gaurus and Bos javanicus |
description |
Since the second Indochina war, habitat destruction and overhunting has resulted in fragmentation of the remaining populations of Bos javanicus and B. gaurus. Nowadays, both species are in serious danger, especially the gaur. In Vietnam, where these species have become almost impossible to capture in the wild, noninvasive investigations are the only feasible approach to obtain data on populations. However, non-invasive derived DNA, especially in tropical areas, is usually characterized by low concentrations, poor quality and/or contamination from alien DNA. To assist in tropical conservation management, baseline information is provided here on assessing the reliability of species identification, molecular sexing and microsatellite genotyping using fecal DNA from B. gaurus and B. javanicus. For species identification using bovine fecal samples, cytochrome b fragment between positions 867 and 1140 was found to contain species diagnostic sites, which distinguishes the four species encountered in the region: B. gaurus, B. indicus, B. javanicus and B. taurus. For sex determination, primers were initially tested on DNA obtained from blood. Then, these primers were successfully used on DNA derived from fecal material. Finally, we also evaluate the feasibility of non-invasive microsatellite genotyping on fecal samples collected in Vietnamese nature reserves. The results presented here improve on current molecular methods based on fecal material obtained from tropical areas. |
format |
article |
topic_facet |
U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes L20 - Écologie animale L60 - Taxonomie et géographie animales gaur Banteng animal sauvage http://aims.fao.org/aos/agrovoc/c_3205 http://aims.fao.org/aos/agrovoc/c_26758 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_8227 |
author |
Rivière-Dobigny, Taiana Pham Doan Lan, Le Quang Nam, Maillard, Jean-Charles Michaux, Johan |
author_facet |
Rivière-Dobigny, Taiana Pham Doan Lan, Le Quang Nam, Maillard, Jean-Charles Michaux, Johan |
author_sort |
Rivière-Dobigny, Taiana |
title |
Species identification, molecular sexing and genotyping using non-invasive approaches in two wild bovids species: Bos gaurus and Bos javanicus |
title_short |
Species identification, molecular sexing and genotyping using non-invasive approaches in two wild bovids species: Bos gaurus and Bos javanicus |
title_full |
Species identification, molecular sexing and genotyping using non-invasive approaches in two wild bovids species: Bos gaurus and Bos javanicus |
title_fullStr |
Species identification, molecular sexing and genotyping using non-invasive approaches in two wild bovids species: Bos gaurus and Bos javanicus |
title_full_unstemmed |
Species identification, molecular sexing and genotyping using non-invasive approaches in two wild bovids species: Bos gaurus and Bos javanicus |
title_sort |
species identification, molecular sexing and genotyping using non-invasive approaches in two wild bovids species: bos gaurus and bos javanicus |
url |
http://agritrop.cirad.fr/548673/ http://agritrop.cirad.fr/548673/1/document_548673.pdf |
work_keys_str_mv |
AT rivieredobignytaiana speciesidentificationmolecularsexingandgenotypingusingnoninvasiveapproachesintwowildbovidsspeciesbosgaurusandbosjavanicus AT phamdoanlan speciesidentificationmolecularsexingandgenotypingusingnoninvasiveapproachesintwowildbovidsspeciesbosgaurusandbosjavanicus AT lequangnam speciesidentificationmolecularsexingandgenotypingusingnoninvasiveapproachesintwowildbovidsspeciesbosgaurusandbosjavanicus AT maillardjeancharles speciesidentificationmolecularsexingandgenotypingusingnoninvasiveapproachesintwowildbovidsspeciesbosgaurusandbosjavanicus AT michauxjohan speciesidentificationmolecularsexingandgenotypingusingnoninvasiveapproachesintwowildbovidsspeciesbosgaurusandbosjavanicus |
_version_ |
1792497160731754496 |