Use of SSR markers to determine the anther-derived homozygous lines in coconut
Anther culture was used to obtain dihaploid (DH) coconut plants and their ploidy level was determined by flow cytometric analysis. Simple sequence repeat (SSR) marker analysis was conducted to identify the homozygous diploid individuals. Ploidy analysis showed that 50% of the tested plantlets were haploid and 50% were diploid. Polymorphic fragments of the mother palm and their segregation patterns in anther-derived plantlets were used to determine the origin of the diploid plantlets. Using a diagnostic SSR marker (CNZ43), all the diploid plantlets tested were identified as being derived from microspores (i.e. were homozygous) and were thus candidates for use in coconut breeding programs.
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Subjects: | F30 - Génétique et amélioration des plantes, F02 - Multiplication végétative des plantes, Cocos nucifera, culture d'anthère, dihaploïdie, marqueur génétique, identification, adn, http://aims.fao.org/aos/agrovoc/c_1716, http://aims.fao.org/aos/agrovoc/c_8837, http://aims.fao.org/aos/agrovoc/c_34237, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_2347, |
Online Access: | http://agritrop.cirad.fr/546870/ http://agritrop.cirad.fr/546870/1/document_546870.pdf |
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dig-cirad-fr-5468702024-01-28T16:30:10Z http://agritrop.cirad.fr/546870/ http://agritrop.cirad.fr/546870/ Use of SSR markers to determine the anther-derived homozygous lines in coconut. Perera Prasanthi I.P., Perera Lalith, Hocher Valérie, Verdeil Jean-Luc, Yakandawala Deepthi M.D., Weerakoon L. Kaushalya. 2008. Plant Cell Reports, 27 (11) : 1697-1703.https://doi.org/10.1007/s00299-008-0592-z <https://doi.org/10.1007/s00299-008-0592-z> Use of SSR markers to determine the anther-derived homozygous lines in coconut Perera, Prasanthi I.P. Perera, Lalith Hocher, Valérie Verdeil, Jean-Luc Yakandawala, Deepthi M.D. Weerakoon, L. Kaushalya eng 2008 Plant Cell Reports F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes Cocos nucifera culture d'anthère dihaploïdie marqueur génétique identification adn http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_8837 http://aims.fao.org/aos/agrovoc/c_34237 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_2347 Anther culture was used to obtain dihaploid (DH) coconut plants and their ploidy level was determined by flow cytometric analysis. Simple sequence repeat (SSR) marker analysis was conducted to identify the homozygous diploid individuals. Ploidy analysis showed that 50% of the tested plantlets were haploid and 50% were diploid. Polymorphic fragments of the mother palm and their segregation patterns in anther-derived plantlets were used to determine the origin of the diploid plantlets. Using a diagnostic SSR marker (CNZ43), all the diploid plantlets tested were identified as being derived from microspores (i.e. were homozygous) and were thus candidates for use in coconut breeding programs. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/546870/1/document_546870.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s00299-008-0592-z 10.1007/s00299-008-0592-z info:eu-repo/semantics/altIdentifier/doi/10.1007/s00299-008-0592-z info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s00299-008-0592-z |
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F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes Cocos nucifera culture d'anthère dihaploïdie marqueur génétique identification adn http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_8837 http://aims.fao.org/aos/agrovoc/c_34237 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_2347 F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes Cocos nucifera culture d'anthère dihaploïdie marqueur génétique identification adn http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_8837 http://aims.fao.org/aos/agrovoc/c_34237 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_2347 |
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F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes Cocos nucifera culture d'anthère dihaploïdie marqueur génétique identification adn http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_8837 http://aims.fao.org/aos/agrovoc/c_34237 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_2347 F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes Cocos nucifera culture d'anthère dihaploïdie marqueur génétique identification adn http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_8837 http://aims.fao.org/aos/agrovoc/c_34237 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_2347 Perera, Prasanthi I.P. Perera, Lalith Hocher, Valérie Verdeil, Jean-Luc Yakandawala, Deepthi M.D. Weerakoon, L. Kaushalya Use of SSR markers to determine the anther-derived homozygous lines in coconut |
description |
Anther culture was used to obtain dihaploid (DH) coconut plants and their ploidy level was determined by flow cytometric analysis. Simple sequence repeat (SSR) marker analysis was conducted to identify the homozygous diploid individuals. Ploidy analysis showed that 50% of the tested plantlets were haploid and 50% were diploid. Polymorphic fragments of the mother palm and their segregation patterns in anther-derived plantlets were used to determine the origin of the diploid plantlets. Using a diagnostic SSR marker (CNZ43), all the diploid plantlets tested were identified as being derived from microspores (i.e. were homozygous) and were thus candidates for use in coconut breeding programs. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes Cocos nucifera culture d'anthère dihaploïdie marqueur génétique identification adn http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_8837 http://aims.fao.org/aos/agrovoc/c_34237 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_2347 |
author |
Perera, Prasanthi I.P. Perera, Lalith Hocher, Valérie Verdeil, Jean-Luc Yakandawala, Deepthi M.D. Weerakoon, L. Kaushalya |
author_facet |
Perera, Prasanthi I.P. Perera, Lalith Hocher, Valérie Verdeil, Jean-Luc Yakandawala, Deepthi M.D. Weerakoon, L. Kaushalya |
author_sort |
Perera, Prasanthi I.P. |
title |
Use of SSR markers to determine the anther-derived homozygous lines in coconut |
title_short |
Use of SSR markers to determine the anther-derived homozygous lines in coconut |
title_full |
Use of SSR markers to determine the anther-derived homozygous lines in coconut |
title_fullStr |
Use of SSR markers to determine the anther-derived homozygous lines in coconut |
title_full_unstemmed |
Use of SSR markers to determine the anther-derived homozygous lines in coconut |
title_sort |
use of ssr markers to determine the anther-derived homozygous lines in coconut |
url |
http://agritrop.cirad.fr/546870/ http://agritrop.cirad.fr/546870/1/document_546870.pdf |
work_keys_str_mv |
AT pereraprasanthiip useofssrmarkerstodeterminetheantherderivedhomozygouslinesincoconut AT pereralalith useofssrmarkerstodeterminetheantherderivedhomozygouslinesincoconut AT hochervalerie useofssrmarkerstodeterminetheantherderivedhomozygouslinesincoconut AT verdeiljeanluc useofssrmarkerstodeterminetheantherderivedhomozygouslinesincoconut AT yakandawaladeepthimd useofssrmarkerstodeterminetheantherderivedhomozygouslinesincoconut AT weerakoonlkaushalya useofssrmarkerstodeterminetheantherderivedhomozygouslinesincoconut |
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1792497041745641472 |