Development of microsatellite markers in Rhinanthus angustifolius and cross-species amplification
Fifteen polymorphic microsatellite loci were developed from an enriched genomic library of the annual plant Rhinanthus angustifolius and characterized using 36 individuals. These markers provided high polymorphism ranging from two to 15 alleles per locus. Four loci showed significant departure from Hardy-Weinberg equilibrium, probably because of the occurrence of null alleles. No significant linkage disequilibrium was detected between pairs of loci. Tests of cross-species transferability were performed on four congeners with a success rate of 100% in Rhinanthus minor, 93% in R. mediterraneus and R. glacialis, and 80% in R. alectorolophus. These microsatellite loci will be useful tools to study mating system, gene flow and hybridization in the genus Rhinanthus.
Autores principales: | , , |
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Formato: | article biblioteca |
Idioma: | eng |
Materias: | F30 - Génétique et amélioration des plantes, Orobanchaceae, microsatellite, marqueur génétique, identification, polymorphisme génétique, collection de matériel génétique, http://aims.fao.org/aos/agrovoc/c_5426, http://aims.fao.org/aos/agrovoc/c_36574, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_24031, http://aims.fao.org/aos/agrovoc/c_36739, |
Acceso en línea: | http://agritrop.cirad.fr/543222/ http://agritrop.cirad.fr/543222/1/543222.pdf |
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dig-cirad-fr-5432222024-01-28T15:38:10Z http://agritrop.cirad.fr/543222/ http://agritrop.cirad.fr/543222/ Development of microsatellite markers in Rhinanthus angustifolius and cross-species amplification. Ducarme V., Risterucci Ange-Marie, Wesselingh R.A.. 2008. Molecular Ecology Resources, 8 (2) : 384-386.https://doi.org/10.1111/j.1471-8286.2007.01966.x <https://doi.org/10.1111/j.1471-8286.2007.01966.x> Development of microsatellite markers in Rhinanthus angustifolius and cross-species amplification Ducarme, V. Risterucci, Ange-Marie Wesselingh, R.A. eng 2008 Molecular Ecology Resources F30 - Génétique et amélioration des plantes Orobanchaceae microsatellite marqueur génétique identification polymorphisme génétique collection de matériel génétique http://aims.fao.org/aos/agrovoc/c_5426 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_36739 Fifteen polymorphic microsatellite loci were developed from an enriched genomic library of the annual plant Rhinanthus angustifolius and characterized using 36 individuals. These markers provided high polymorphism ranging from two to 15 alleles per locus. Four loci showed significant departure from Hardy-Weinberg equilibrium, probably because of the occurrence of null alleles. No significant linkage disequilibrium was detected between pairs of loci. Tests of cross-species transferability were performed on four congeners with a success rate of 100% in Rhinanthus minor, 93% in R. mediterraneus and R. glacialis, and 80% in R. alectorolophus. These microsatellite loci will be useful tools to study mating system, gene flow and hybridization in the genus Rhinanthus. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/543222/1/543222.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1111/j.1471-8286.2007.01966.x 10.1111/j.1471-8286.2007.01966.x http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=199300 info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-8286.2007.01966.x info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1111/j.1471-8286.2007.01966.x |
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F30 - Génétique et amélioration des plantes Orobanchaceae microsatellite marqueur génétique identification polymorphisme génétique collection de matériel génétique http://aims.fao.org/aos/agrovoc/c_5426 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_36739 F30 - Génétique et amélioration des plantes Orobanchaceae microsatellite marqueur génétique identification polymorphisme génétique collection de matériel génétique http://aims.fao.org/aos/agrovoc/c_5426 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_36739 |
spellingShingle |
F30 - Génétique et amélioration des plantes Orobanchaceae microsatellite marqueur génétique identification polymorphisme génétique collection de matériel génétique http://aims.fao.org/aos/agrovoc/c_5426 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_36739 F30 - Génétique et amélioration des plantes Orobanchaceae microsatellite marqueur génétique identification polymorphisme génétique collection de matériel génétique http://aims.fao.org/aos/agrovoc/c_5426 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_36739 Ducarme, V. Risterucci, Ange-Marie Wesselingh, R.A. Development of microsatellite markers in Rhinanthus angustifolius and cross-species amplification |
description |
Fifteen polymorphic microsatellite loci were developed from an enriched genomic library of the annual plant Rhinanthus angustifolius and characterized using 36 individuals. These markers provided high polymorphism ranging from two to 15 alleles per locus. Four loci showed significant departure from Hardy-Weinberg equilibrium, probably because of the occurrence of null alleles. No significant linkage disequilibrium was detected between pairs of loci. Tests of cross-species transferability were performed on four congeners with a success rate of 100% in Rhinanthus minor, 93% in R. mediterraneus and R. glacialis, and 80% in R. alectorolophus. These microsatellite loci will be useful tools to study mating system, gene flow and hybridization in the genus Rhinanthus. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes Orobanchaceae microsatellite marqueur génétique identification polymorphisme génétique collection de matériel génétique http://aims.fao.org/aos/agrovoc/c_5426 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_36739 |
author |
Ducarme, V. Risterucci, Ange-Marie Wesselingh, R.A. |
author_facet |
Ducarme, V. Risterucci, Ange-Marie Wesselingh, R.A. |
author_sort |
Ducarme, V. |
title |
Development of microsatellite markers in Rhinanthus angustifolius and cross-species amplification |
title_short |
Development of microsatellite markers in Rhinanthus angustifolius and cross-species amplification |
title_full |
Development of microsatellite markers in Rhinanthus angustifolius and cross-species amplification |
title_fullStr |
Development of microsatellite markers in Rhinanthus angustifolius and cross-species amplification |
title_full_unstemmed |
Development of microsatellite markers in Rhinanthus angustifolius and cross-species amplification |
title_sort |
development of microsatellite markers in rhinanthus angustifolius and cross-species amplification |
url |
http://agritrop.cirad.fr/543222/ http://agritrop.cirad.fr/543222/1/543222.pdf |
work_keys_str_mv |
AT ducarmev developmentofmicrosatellitemarkersinrhinanthusangustifoliusandcrossspeciesamplification AT risterucciangemarie developmentofmicrosatellitemarkersinrhinanthusangustifoliusandcrossspeciesamplification AT wesselinghra developmentofmicrosatellitemarkersinrhinanthusangustifoliusandcrossspeciesamplification |
_version_ |
1792496796975497216 |