Cycle characteristics in a temporary immersion bioreactor affect regeneration, morphology, water and mineral status of coffee (Coffea arabica) somatic embryos
Mass regeneration of Coffea arabica L. somatic embryos using a temporary immersion bioreactor was improved by optimizing the immersion cycles, i.e. both the duration and the frequency of immersions. It was demonstrated that increasing the frequency of short immersions (1 min immersions every 24, 12 and 4 h) stimulated embryo production (480, 2,094 and 3,081 embryos/1-1 bioreactor, respectively) and improved quality (60, 79 and 85% of torpedo shaped embryos, respectively). On the other hand, an increase in the immersion duration (1, 5 and 15 min) inhibited embryo regeneration (from 2,094 to 428 embryos per 1-1 bioreactor) and negatively affected their morphological quality (from 79 to 49% torpedo-shaped embryos) and the conversion of embryos into plants (from 70 to 33%). A 15 min immersion duration applied every 4 h produced hyperhydric symptoms in 90% of the embryos. Hyperhydric embryos were characterized by higher fresh weight and water content, more negative values for water potential and higher K+ content when compared to normal torpedo-shaped embryos. Micrographs showed structural problems in the globular stage, such as the existence of an irregular epidermis and an absence of reserves. Whatever the immersion cycle used, the somatic embryos exhibited water and mineral characteristics very different from those of their zygotic counterparts. The use of 1 min immersions every 4 h led to the production of the largest quantities of torpedo-shaped embryos without hyperhydricity that succeeded in regenerating plants (75% conversion).
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Subjects: | F02 - Multiplication végétative des plantes, Coffea arabica, http://aims.fao.org/aos/agrovoc/c_1721, http://aims.fao.org/aos/agrovoc/c_1070, http://aims.fao.org/aos/agrovoc/c_2676, |
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dig-cirad-fr-5318262023-06-19T16:03:35Z http://agritrop.cirad.fr/531826/ http://agritrop.cirad.fr/531826/ Cycle characteristics in a temporary immersion bioreactor affect regeneration, morphology, water and mineral status of coffee (Coffea arabica) somatic embryos. Albarran J., Bertrand Benoît, Lartaud Marc, Etienne Hervé. 2005. Plant Cell, Tissue and Organ Culture, 81 : 27-36.https://doi.org/10.1007/s11240-004-2618-8 <https://doi.org/10.1007/s11240-004-2618-8> Researchers Cycle characteristics in a temporary immersion bioreactor affect regeneration, morphology, water and mineral status of coffee (Coffea arabica) somatic embryos Albarran, J. Bertrand, Benoît Lartaud, Marc Etienne, Hervé eng 2005 Plant Cell, Tissue and Organ Culture F02 - Multiplication végétative des plantes Coffea arabica http://aims.fao.org/aos/agrovoc/c_1721 Brésil Éthiopie http://aims.fao.org/aos/agrovoc/c_1070 http://aims.fao.org/aos/agrovoc/c_2676 Mass regeneration of Coffea arabica L. somatic embryos using a temporary immersion bioreactor was improved by optimizing the immersion cycles, i.e. both the duration and the frequency of immersions. It was demonstrated that increasing the frequency of short immersions (1 min immersions every 24, 12 and 4 h) stimulated embryo production (480, 2,094 and 3,081 embryos/1-1 bioreactor, respectively) and improved quality (60, 79 and 85% of torpedo shaped embryos, respectively). On the other hand, an increase in the immersion duration (1, 5 and 15 min) inhibited embryo regeneration (from 2,094 to 428 embryos per 1-1 bioreactor) and negatively affected their morphological quality (from 79 to 49% torpedo-shaped embryos) and the conversion of embryos into plants (from 70 to 33%). A 15 min immersion duration applied every 4 h produced hyperhydric symptoms in 90% of the embryos. Hyperhydric embryos were characterized by higher fresh weight and water content, more negative values for water potential and higher K+ content when compared to normal torpedo-shaped embryos. Micrographs showed structural problems in the globular stage, such as the existence of an irregular epidermis and an absence of reserves. Whatever the immersion cycle used, the somatic embryos exhibited water and mineral characteristics very different from those of their zygotic counterparts. The use of 1 min immersions every 4 h led to the production of the largest quantities of torpedo-shaped embryos without hyperhydricity that succeeded in regenerating plants (75% conversion). article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/531826/1/document_531826.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s11240-004-2618-8 10.1007/s11240-004-2618-8 info:eu-repo/semantics/altIdentifier/doi/10.1007/s11240-004-2618-8 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s11240-004-2618-8 |
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F02 - Multiplication végétative des plantes Coffea arabica http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_1070 http://aims.fao.org/aos/agrovoc/c_2676 F02 - Multiplication végétative des plantes Coffea arabica http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_1070 http://aims.fao.org/aos/agrovoc/c_2676 |
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F02 - Multiplication végétative des plantes Coffea arabica http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_1070 http://aims.fao.org/aos/agrovoc/c_2676 F02 - Multiplication végétative des plantes Coffea arabica http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_1070 http://aims.fao.org/aos/agrovoc/c_2676 Albarran, J. Bertrand, Benoît Lartaud, Marc Etienne, Hervé Cycle characteristics in a temporary immersion bioreactor affect regeneration, morphology, water and mineral status of coffee (Coffea arabica) somatic embryos |
description |
Mass regeneration of Coffea arabica L. somatic embryos using a temporary immersion bioreactor was improved by optimizing the immersion cycles, i.e. both the duration and the frequency of immersions. It was demonstrated that increasing the frequency of short immersions (1 min immersions every 24, 12 and 4 h) stimulated embryo production (480, 2,094 and 3,081 embryos/1-1 bioreactor, respectively) and improved quality (60, 79 and 85% of torpedo shaped embryos, respectively). On the other hand, an increase in the immersion duration (1, 5 and 15 min) inhibited embryo regeneration (from 2,094 to 428 embryos per 1-1 bioreactor) and negatively affected their morphological quality (from 79 to 49% torpedo-shaped embryos) and the conversion of embryos into plants (from 70 to 33%). A 15 min immersion duration applied every 4 h produced hyperhydric symptoms in 90% of the embryos. Hyperhydric embryos were characterized by higher fresh weight and water content, more negative values for water potential and higher K+ content when compared to normal torpedo-shaped embryos. Micrographs showed structural problems in the globular stage, such as the existence of an irregular epidermis and an absence of reserves. Whatever the immersion cycle used, the somatic embryos exhibited water and mineral characteristics very different from those of their zygotic counterparts. The use of 1 min immersions every 4 h led to the production of the largest quantities of torpedo-shaped embryos without hyperhydricity that succeeded in regenerating plants (75% conversion). |
format |
article |
topic_facet |
F02 - Multiplication végétative des plantes Coffea arabica http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_1070 http://aims.fao.org/aos/agrovoc/c_2676 |
author |
Albarran, J. Bertrand, Benoît Lartaud, Marc Etienne, Hervé |
author_facet |
Albarran, J. Bertrand, Benoît Lartaud, Marc Etienne, Hervé |
author_sort |
Albarran, J. |
title |
Cycle characteristics in a temporary immersion bioreactor affect regeneration, morphology, water and mineral status of coffee (Coffea arabica) somatic embryos |
title_short |
Cycle characteristics in a temporary immersion bioreactor affect regeneration, morphology, water and mineral status of coffee (Coffea arabica) somatic embryos |
title_full |
Cycle characteristics in a temporary immersion bioreactor affect regeneration, morphology, water and mineral status of coffee (Coffea arabica) somatic embryos |
title_fullStr |
Cycle characteristics in a temporary immersion bioreactor affect regeneration, morphology, water and mineral status of coffee (Coffea arabica) somatic embryos |
title_full_unstemmed |
Cycle characteristics in a temporary immersion bioreactor affect regeneration, morphology, water and mineral status of coffee (Coffea arabica) somatic embryos |
title_sort |
cycle characteristics in a temporary immersion bioreactor affect regeneration, morphology, water and mineral status of coffee (coffea arabica) somatic embryos |
url |
http://agritrop.cirad.fr/531826/ http://agritrop.cirad.fr/531826/1/document_531826.pdf |
work_keys_str_mv |
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1769605072092659712 |