Ectomycorrhizal symbiosis enhanced the efficiency of inoculation with two Bradyrhizobium staims and Acacia holosericea growth
Two strains of Bradyrhizobium sp., Aust 13C and Aust 11C, were dually or singly inoculated with an ectomycorrhizal fungus, Pisolithus albus to assess the interactions between ectomycorrhizal symbiosis and the nodulation process in glasshouse conditions. Sequencing of strains Aust 13C and Aust 11C confirmed their previous placement in the genus Bradyrhizobium. After 4 months' culture, the ectomycorrhizal symbiosis promoted plant growth and the nodulation process of both Bradyrhizobium strains, singly or dually inoculated. PCRIRFLP analysis of the nodules randomly collected in each treatment with Aust 13C and/or Aust 11C: (1) showed that all the nodules exhibited the same patterns as those of the Bradyrhizobium strains, and (2) did not detect contaminant rhizobia. When both Bradyrhizobium isolates were inoculated together, but without P. a/bus IR100, Aust 11C was recorded in 13% of the treated nodules compared to 87% for Aust 13C, where-as Aust 11C and Aust 13C were represented in 20 and 80% of the treated nodules, respectively, in the ectomycorrhizal treatment. Therefore Aust 13C had a high competitive ability and a great persistence in soil. The presence of the fungus did not significantly influence the frequencies of each Bradyrhizobium sp. root nodules. Although the mechanisms remain unknown, these results showed that the ectomycorrhizal and biological nitrogen-fixing symbioses were very dependent on each other. From a practical point of view, the role of ectomycorrhizal symbiosis is of great importance to N2 fixation and, consequently, these kinds of symbiosis must be associated in any controlled inoculation.
Main Authors: | , , , , , |
---|---|
Format: | article biblioteca |
Language: | eng |
Subjects: | P34 - Biologie du sol, F62 - Physiologie végétale - Croissance et développement, Bradyrhizobium, Acacia holosericea, Pisolithus, symbiose, nodosité racinaire, Rhizobium, Mycorhizé, inoculation, bactérie fixatrice de l'azote, http://aims.fao.org/aos/agrovoc/c_27138, http://aims.fao.org/aos/agrovoc/c_26329, http://aims.fao.org/aos/agrovoc/c_23961, http://aims.fao.org/aos/agrovoc/c_7563, http://aims.fao.org/aos/agrovoc/c_27601, http://aims.fao.org/aos/agrovoc/c_6563, http://aims.fao.org/aos/agrovoc/c_5023, http://aims.fao.org/aos/agrovoc/c_3879, http://aims.fao.org/aos/agrovoc/c_27939, |
Online Access: | http://agritrop.cirad.fr/528515/ http://agritrop.cirad.fr/528515/1/document_528515.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-528515 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5285152024-01-28T13:55:06Z http://agritrop.cirad.fr/528515/ http://agritrop.cirad.fr/528515/ Ectomycorrhizal symbiosis enhanced the efficiency of inoculation with two Bradyrhizobium staims and Acacia holosericea growth. André S., Galiana Antoine, Le Roux Christine, Prin Yves, Neyra Marc, Duponnois Robin. 2005. Mycorrhiza, 15 : 357-364.https://doi.org/10.1007/s00572-004-0340-3 <https://doi.org/10.1007/s00572-004-0340-3> Ectomycorrhizal symbiosis enhanced the efficiency of inoculation with two Bradyrhizobium staims and Acacia holosericea growth André, S. Galiana, Antoine Le Roux, Christine Prin, Yves Neyra, Marc Duponnois, Robin eng 2005 Mycorrhiza P34 - Biologie du sol F62 - Physiologie végétale - Croissance et développement Bradyrhizobium Acacia holosericea Pisolithus symbiose nodosité racinaire Rhizobium Mycorhizé inoculation bactérie fixatrice de l'azote http://aims.fao.org/aos/agrovoc/c_27138 http://aims.fao.org/aos/agrovoc/c_26329 http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_27601 http://aims.fao.org/aos/agrovoc/c_6563 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_3879 http://aims.fao.org/aos/agrovoc/c_27939 Two strains of Bradyrhizobium sp., Aust 13C and Aust 11C, were dually or singly inoculated with an ectomycorrhizal fungus, Pisolithus albus to assess the interactions between ectomycorrhizal symbiosis and the nodulation process in glasshouse conditions. Sequencing of strains Aust 13C and Aust 11C confirmed their previous placement in the genus Bradyrhizobium. After 4 months' culture, the ectomycorrhizal symbiosis promoted plant growth and the nodulation process of both Bradyrhizobium strains, singly or dually inoculated. PCRIRFLP analysis of the nodules randomly collected in each treatment with Aust 13C and/or Aust 11C: (1) showed that all the nodules exhibited the same patterns as those of the Bradyrhizobium strains, and (2) did not detect contaminant rhizobia. When both Bradyrhizobium isolates were inoculated together, but without P. a/bus IR100, Aust 11C was recorded in 13% of the treated nodules compared to 87% for Aust 13C, where-as Aust 11C and Aust 13C were represented in 20 and 80% of the treated nodules, respectively, in the ectomycorrhizal treatment. Therefore Aust 13C had a high competitive ability and a great persistence in soil. The presence of the fungus did not significantly influence the frequencies of each Bradyrhizobium sp. root nodules. Although the mechanisms remain unknown, these results showed that the ectomycorrhizal and biological nitrogen-fixing symbioses were very dependent on each other. From a practical point of view, the role of ectomycorrhizal symbiosis is of great importance to N2 fixation and, consequently, these kinds of symbiosis must be associated in any controlled inoculation. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/528515/1/document_528515.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s00572-004-0340-3 10.1007/s00572-004-0340-3 info:eu-repo/semantics/altIdentifier/doi/10.1007/s00572-004-0340-3 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s00572-004-0340-3 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
P34 - Biologie du sol F62 - Physiologie végétale - Croissance et développement Bradyrhizobium Acacia holosericea Pisolithus symbiose nodosité racinaire Rhizobium Mycorhizé inoculation bactérie fixatrice de l'azote http://aims.fao.org/aos/agrovoc/c_27138 http://aims.fao.org/aos/agrovoc/c_26329 http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_27601 http://aims.fao.org/aos/agrovoc/c_6563 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_3879 http://aims.fao.org/aos/agrovoc/c_27939 P34 - Biologie du sol F62 - Physiologie végétale - Croissance et développement Bradyrhizobium Acacia holosericea Pisolithus symbiose nodosité racinaire Rhizobium Mycorhizé inoculation bactérie fixatrice de l'azote http://aims.fao.org/aos/agrovoc/c_27138 http://aims.fao.org/aos/agrovoc/c_26329 http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_27601 http://aims.fao.org/aos/agrovoc/c_6563 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_3879 http://aims.fao.org/aos/agrovoc/c_27939 |
spellingShingle |
P34 - Biologie du sol F62 - Physiologie végétale - Croissance et développement Bradyrhizobium Acacia holosericea Pisolithus symbiose nodosité racinaire Rhizobium Mycorhizé inoculation bactérie fixatrice de l'azote http://aims.fao.org/aos/agrovoc/c_27138 http://aims.fao.org/aos/agrovoc/c_26329 http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_27601 http://aims.fao.org/aos/agrovoc/c_6563 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_3879 http://aims.fao.org/aos/agrovoc/c_27939 P34 - Biologie du sol F62 - Physiologie végétale - Croissance et développement Bradyrhizobium Acacia holosericea Pisolithus symbiose nodosité racinaire Rhizobium Mycorhizé inoculation bactérie fixatrice de l'azote http://aims.fao.org/aos/agrovoc/c_27138 http://aims.fao.org/aos/agrovoc/c_26329 http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_27601 http://aims.fao.org/aos/agrovoc/c_6563 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_3879 http://aims.fao.org/aos/agrovoc/c_27939 André, S. Galiana, Antoine Le Roux, Christine Prin, Yves Neyra, Marc Duponnois, Robin Ectomycorrhizal symbiosis enhanced the efficiency of inoculation with two Bradyrhizobium staims and Acacia holosericea growth |
description |
Two strains of Bradyrhizobium sp., Aust 13C and Aust 11C, were dually or singly inoculated with an ectomycorrhizal fungus, Pisolithus albus to assess the interactions between ectomycorrhizal symbiosis and the nodulation process in glasshouse conditions. Sequencing of strains Aust 13C and Aust 11C confirmed their previous placement in the genus Bradyrhizobium. After 4 months' culture, the ectomycorrhizal symbiosis promoted plant growth and the nodulation process of both Bradyrhizobium strains, singly or dually inoculated. PCRIRFLP analysis of the nodules randomly collected in each treatment with Aust 13C and/or Aust 11C: (1) showed that all the nodules exhibited the same patterns as those of the Bradyrhizobium strains, and (2) did not detect contaminant rhizobia. When both Bradyrhizobium isolates were inoculated together, but without P. a/bus IR100, Aust 11C was recorded in 13% of the treated nodules compared to 87% for Aust 13C, where-as Aust 11C and Aust 13C were represented in 20 and 80% of the treated nodules, respectively, in the ectomycorrhizal treatment. Therefore Aust 13C had a high competitive ability and a great persistence in soil. The presence of the fungus did not significantly influence the frequencies of each Bradyrhizobium sp. root nodules. Although the mechanisms remain unknown, these results showed that the ectomycorrhizal and biological nitrogen-fixing symbioses were very dependent on each other. From a practical point of view, the role of ectomycorrhizal symbiosis is of great importance to N2 fixation and, consequently, these kinds of symbiosis must be associated in any controlled inoculation. |
format |
article |
topic_facet |
P34 - Biologie du sol F62 - Physiologie végétale - Croissance et développement Bradyrhizobium Acacia holosericea Pisolithus symbiose nodosité racinaire Rhizobium Mycorhizé inoculation bactérie fixatrice de l'azote http://aims.fao.org/aos/agrovoc/c_27138 http://aims.fao.org/aos/agrovoc/c_26329 http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_27601 http://aims.fao.org/aos/agrovoc/c_6563 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_3879 http://aims.fao.org/aos/agrovoc/c_27939 |
author |
André, S. Galiana, Antoine Le Roux, Christine Prin, Yves Neyra, Marc Duponnois, Robin |
author_facet |
André, S. Galiana, Antoine Le Roux, Christine Prin, Yves Neyra, Marc Duponnois, Robin |
author_sort |
André, S. |
title |
Ectomycorrhizal symbiosis enhanced the efficiency of inoculation with two Bradyrhizobium staims and Acacia holosericea growth |
title_short |
Ectomycorrhizal symbiosis enhanced the efficiency of inoculation with two Bradyrhizobium staims and Acacia holosericea growth |
title_full |
Ectomycorrhizal symbiosis enhanced the efficiency of inoculation with two Bradyrhizobium staims and Acacia holosericea growth |
title_fullStr |
Ectomycorrhizal symbiosis enhanced the efficiency of inoculation with two Bradyrhizobium staims and Acacia holosericea growth |
title_full_unstemmed |
Ectomycorrhizal symbiosis enhanced the efficiency of inoculation with two Bradyrhizobium staims and Acacia holosericea growth |
title_sort |
ectomycorrhizal symbiosis enhanced the efficiency of inoculation with two bradyrhizobium staims and acacia holosericea growth |
url |
http://agritrop.cirad.fr/528515/ http://agritrop.cirad.fr/528515/1/document_528515.pdf |
work_keys_str_mv |
AT andres ectomycorrhizalsymbiosisenhancedtheefficiencyofinoculationwithtwobradyrhizobiumstaimsandacaciaholosericeagrowth AT galianaantoine ectomycorrhizalsymbiosisenhancedtheefficiencyofinoculationwithtwobradyrhizobiumstaimsandacaciaholosericeagrowth AT lerouxchristine ectomycorrhizalsymbiosisenhancedtheefficiencyofinoculationwithtwobradyrhizobiumstaimsandacaciaholosericeagrowth AT prinyves ectomycorrhizalsymbiosisenhancedtheefficiencyofinoculationwithtwobradyrhizobiumstaimsandacaciaholosericeagrowth AT neyramarc ectomycorrhizalsymbiosisenhancedtheefficiencyofinoculationwithtwobradyrhizobiumstaimsandacaciaholosericeagrowth AT duponnoisrobin ectomycorrhizalsymbiosisenhancedtheefficiencyofinoculationwithtwobradyrhizobiumstaimsandacaciaholosericeagrowth |
_version_ |
1792496275976880128 |