Coconut microsatellite markers and their use in genebank organisation and genetic mapping
Characterisation and evaluation of coconut populations, based on morphological and agronomic traits is time-consuming, labour-intensive and subject to environmental effects. Some 1200 accessions are recognised by the International Coconut Genetic Resources Network (COGENT) and they plan to collect threatened and useful germplasm to augment the collections, with a view to establishing regional gene-banks. Therefore, sensitive and reliable techniques for assessing genetic diversity and screening agronomic traits are necessary to establish effective collecting and gene-banking strategies. A set of 37 highly polymorphic microsatellite markers were isolated from a small-insert microsatellite-enriched library of the cultivar Tagnanan Tall (Rivera et al., 1999. Genome 42:668-675). In a recent study, 31 individuals from 14 coconut populations across the entire geographic range were tested with the microsatellite markers and 339 alleles were detected (Teulat et al., 1999. Theoretical and Applied Genetics, in press). Generally, diversity was higher in populations from the South Pacific and South East Asia. Three microsatellite loci gave distinct genotypes for all but two populations. East African populations had higher heterozygosities than those from West Africa, and the populations from Tonga and Fiji had distinct alleles from those of the South Pacific. AFLP analysis with 12 primer combinations produced similar relationships and were also in agreement with previous RFLP results obtained with the same material (Lebrun et al., 1998. Euphytica 101:103-108). In collaboration with five European laboratories, we have embarked on a genome mapping project using AFLPs, microsatellites, RFLPs and ISTR markers. Preliminary results from this work will also be discussed. (Texte intégral)
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Subjects: | F30 - Génétique et amélioration des plantes, Cocos nucifera, marqueur génétique, microsatellite, carte génétique, variation génétique, distribution géographique, RFLP, http://aims.fao.org/aos/agrovoc/c_1716, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_36574, http://aims.fao.org/aos/agrovoc/c_24002, http://aims.fao.org/aos/agrovoc/c_15975, http://aims.fao.org/aos/agrovoc/c_5083, http://aims.fao.org/aos/agrovoc/c_34255, |
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dig-cirad-fr-5122952024-01-28T11:08:17Z http://agritrop.cirad.fr/512295/ http://agritrop.cirad.fr/512295/ Coconut microsatellite markers and their use in genebank organisation and genetic mapping. Barker Jacqueline H.A., Teulat Béatrice, Lebrun Patricia, Baudouin Luc, Karp Angela, Rognon François. 2000. . s.l. : s.n., Résumé Plant and Animal Genomes Conference. 8, San Diego, États-Unis, 9 Janvier 2000/12 Janvier 2000.http://www.intl-pag.org/8/abstracts/pag8365.html <http://www.intl-pag.org/8/abstracts/pag8365.html> Coconut microsatellite markers and their use in genebank organisation and genetic mapping Barker, Jacqueline H.A. Teulat, Béatrice Lebrun, Patricia Baudouin, Luc Karp, Angela Rognon, François eng 2000 s.n. F30 - Génétique et amélioration des plantes Cocos nucifera marqueur génétique microsatellite carte génétique variation génétique distribution géographique RFLP http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_5083 http://aims.fao.org/aos/agrovoc/c_34255 Characterisation and evaluation of coconut populations, based on morphological and agronomic traits is time-consuming, labour-intensive and subject to environmental effects. Some 1200 accessions are recognised by the International Coconut Genetic Resources Network (COGENT) and they plan to collect threatened and useful germplasm to augment the collections, with a view to establishing regional gene-banks. Therefore, sensitive and reliable techniques for assessing genetic diversity and screening agronomic traits are necessary to establish effective collecting and gene-banking strategies. A set of 37 highly polymorphic microsatellite markers were isolated from a small-insert microsatellite-enriched library of the cultivar Tagnanan Tall (Rivera et al., 1999. Genome 42:668-675). In a recent study, 31 individuals from 14 coconut populations across the entire geographic range were tested with the microsatellite markers and 339 alleles were detected (Teulat et al., 1999. Theoretical and Applied Genetics, in press). Generally, diversity was higher in populations from the South Pacific and South East Asia. Three microsatellite loci gave distinct genotypes for all but two populations. East African populations had higher heterozygosities than those from West Africa, and the populations from Tonga and Fiji had distinct alleles from those of the South Pacific. AFLP analysis with 12 primer combinations produced similar relationships and were also in agreement with previous RFLP results obtained with the same material (Lebrun et al., 1998. Euphytica 101:103-108). In collaboration with five European laboratories, we have embarked on a genome mapping project using AFLPs, microsatellites, RFLPs and ISTR markers. Preliminary results from this work will also be discussed. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/closedAccess http://www.intl-pag.org/8/abstracts/pag8365.html http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=176012 info:eu-repo/semantics/altIdentifier/purl/http://www.intl-pag.org/8/abstracts/pag8365.html |
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F30 - Génétique et amélioration des plantes Cocos nucifera marqueur génétique microsatellite carte génétique variation génétique distribution géographique RFLP http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_5083 http://aims.fao.org/aos/agrovoc/c_34255 F30 - Génétique et amélioration des plantes Cocos nucifera marqueur génétique microsatellite carte génétique variation génétique distribution géographique RFLP http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_5083 http://aims.fao.org/aos/agrovoc/c_34255 |
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F30 - Génétique et amélioration des plantes Cocos nucifera marqueur génétique microsatellite carte génétique variation génétique distribution géographique RFLP http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_5083 http://aims.fao.org/aos/agrovoc/c_34255 F30 - Génétique et amélioration des plantes Cocos nucifera marqueur génétique microsatellite carte génétique variation génétique distribution géographique RFLP http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_5083 http://aims.fao.org/aos/agrovoc/c_34255 Barker, Jacqueline H.A. Teulat, Béatrice Lebrun, Patricia Baudouin, Luc Karp, Angela Rognon, François Coconut microsatellite markers and their use in genebank organisation and genetic mapping |
description |
Characterisation and evaluation of coconut populations, based on morphological and agronomic traits is time-consuming, labour-intensive and subject to environmental effects. Some 1200 accessions are recognised by the International Coconut Genetic Resources Network (COGENT) and they plan to collect threatened and useful germplasm to augment the collections, with a view to establishing regional gene-banks. Therefore, sensitive and reliable techniques for assessing genetic diversity and screening agronomic traits are necessary to establish effective collecting and gene-banking strategies. A set of 37 highly polymorphic microsatellite markers were isolated from a small-insert microsatellite-enriched library of the cultivar Tagnanan Tall (Rivera et al., 1999. Genome 42:668-675). In a recent study, 31 individuals from 14 coconut populations across the entire geographic range were tested with the microsatellite markers and 339 alleles were detected (Teulat et al., 1999. Theoretical and Applied Genetics, in press). Generally, diversity was higher in populations from the South Pacific and South East Asia. Three microsatellite loci gave distinct genotypes for all but two populations. East African populations had higher heterozygosities than those from West Africa, and the populations from Tonga and Fiji had distinct alleles from those of the South Pacific. AFLP analysis with 12 primer combinations produced similar relationships and were also in agreement with previous RFLP results obtained with the same material (Lebrun et al., 1998. Euphytica 101:103-108). In collaboration with five European laboratories, we have embarked on a genome mapping project using AFLPs, microsatellites, RFLPs and ISTR markers. Preliminary results from this work will also be discussed. (Texte intégral) |
format |
conference_item |
topic_facet |
F30 - Génétique et amélioration des plantes Cocos nucifera marqueur génétique microsatellite carte génétique variation génétique distribution géographique RFLP http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_5083 http://aims.fao.org/aos/agrovoc/c_34255 |
author |
Barker, Jacqueline H.A. Teulat, Béatrice Lebrun, Patricia Baudouin, Luc Karp, Angela Rognon, François |
author_facet |
Barker, Jacqueline H.A. Teulat, Béatrice Lebrun, Patricia Baudouin, Luc Karp, Angela Rognon, François |
author_sort |
Barker, Jacqueline H.A. |
title |
Coconut microsatellite markers and their use in genebank organisation and genetic mapping |
title_short |
Coconut microsatellite markers and their use in genebank organisation and genetic mapping |
title_full |
Coconut microsatellite markers and their use in genebank organisation and genetic mapping |
title_fullStr |
Coconut microsatellite markers and their use in genebank organisation and genetic mapping |
title_full_unstemmed |
Coconut microsatellite markers and their use in genebank organisation and genetic mapping |
title_sort |
coconut microsatellite markers and their use in genebank organisation and genetic mapping |
publisher |
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url |
http://agritrop.cirad.fr/512295/ |
work_keys_str_mv |
AT barkerjacquelineha coconutmicrosatellitemarkersandtheiruseingenebankorganisationandgeneticmapping AT teulatbeatrice coconutmicrosatellitemarkersandtheiruseingenebankorganisationandgeneticmapping AT lebrunpatricia coconutmicrosatellitemarkersandtheiruseingenebankorganisationandgeneticmapping AT baudouinluc coconutmicrosatellitemarkersandtheiruseingenebankorganisationandgeneticmapping AT karpangela coconutmicrosatellitemarkersandtheiruseingenebankorganisationandgeneticmapping AT rognonfrancois coconutmicrosatellitemarkersandtheiruseingenebankorganisationandgeneticmapping |
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