Viral interference with gene silencing in non-transgenic or transgenic plants
Various agronomically interesting traits have been obtained by inducing post-transcriptional gene silencing (PTGS) in transgenic cultivated crops. Some plant cultivars that were created by interspecific crosses also display PTGS-induced phenotypes. The finding that many plant viruses have the ability to counteract PTGS raises the possibility that viral infection may cause the loss of PTGS-induced traits in transgenic as well as non-transgenic plants. We addressed this question by studying virus-specific differences in the interference with silencing of the chs-A gene in non-transgenic RedStar-type petunia whose flowers display variegated flowers resulting from natural PTGS of the chs-A gene. Our results show that potato potyvirus Y (PVY), tobacco etch potyvirus (TEV) and cucumber mosaic cucumovirus (CMV), which are known to inhibit PTGS, induce specific phenotypic changes resulting from spatially-regulated interference between viral infection and PTGS of the chs-A gene. However, PTGS breakdown was not total, and the pattern observed depended on which virus was involved. This suggests that the degree of interference with PTGS will depend on the pattern of expression of the silenced gene and on that of the accumulation of the potentially interfering virus. We are currently studying the effects of viral infection by TEV or CMV on PTGS-induced resistance to PVY in transgenic tobacco lines expressing a PVY coat protein gene. Preliminary results suggest that the two viruses have different effects. These results show that viral infections could lead to the loss of agronomically interesting traits obtained by PTGS in either transgenic or non-transgenic plants, such as flower color, virus-resistance, or modified seed oil composition.
Main Authors: | , , , |
---|---|
Format: | conference_item biblioteca |
Language: | eng |
Published: |
s.n.
|
Subjects: | H20 - Maladies des plantes, |
Online Access: | http://agritrop.cirad.fr/510776/ |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-510776 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5107762022-04-21T16:24:28Z http://agritrop.cirad.fr/510776/ http://agritrop.cirad.fr/510776/ Viral interference with gene silencing in non-transgenic or transgenic plants. Teycheney Pierre-Yves, Gaubert Stéphane, Balazs Ervin, Tepfer Mark. 2001. In : Working group on genetic interactions, Assessment of the Impacts of Genetically Modified Plants, Portugal, Lisbon, 19-23 september 2001. ESF. s.l. : s.n., 4 p. Assessment of the Impacts of Genetically Modified Plants, Lisbonne, Portugal, 19 Septembre 2001/23 Septembre 2001. Researchers Viral interference with gene silencing in non-transgenic or transgenic plants Teycheney, Pierre-Yves Gaubert, Stéphane Balazs, Ervin Tepfer, Mark eng 2001 s.n. Working group on genetic interactions, Assessment of the Impacts of Genetically Modified Plants, Portugal, Lisbon, 19-23 september 2001 H20 - Maladies des plantes Various agronomically interesting traits have been obtained by inducing post-transcriptional gene silencing (PTGS) in transgenic cultivated crops. Some plant cultivars that were created by interspecific crosses also display PTGS-induced phenotypes. The finding that many plant viruses have the ability to counteract PTGS raises the possibility that viral infection may cause the loss of PTGS-induced traits in transgenic as well as non-transgenic plants. We addressed this question by studying virus-specific differences in the interference with silencing of the chs-A gene in non-transgenic RedStar-type petunia whose flowers display variegated flowers resulting from natural PTGS of the chs-A gene. Our results show that potato potyvirus Y (PVY), tobacco etch potyvirus (TEV) and cucumber mosaic cucumovirus (CMV), which are known to inhibit PTGS, induce specific phenotypic changes resulting from spatially-regulated interference between viral infection and PTGS of the chs-A gene. However, PTGS breakdown was not total, and the pattern observed depended on which virus was involved. This suggests that the degree of interference with PTGS will depend on the pattern of expression of the silenced gene and on that of the accumulation of the potentially interfering virus. We are currently studying the effects of viral infection by TEV or CMV on PTGS-induced resistance to PVY in transgenic tobacco lines expressing a PVY coat protein gene. Preliminary results suggest that the two viruses have different effects. These results show that viral infections could lead to the loss of agronomically interesting traits obtained by PTGS in either transgenic or non-transgenic plants, such as flower color, virus-resistance, or modified seed oil composition. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/closedAccess http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=175048 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
H20 - Maladies des plantes H20 - Maladies des plantes |
spellingShingle |
H20 - Maladies des plantes H20 - Maladies des plantes Teycheney, Pierre-Yves Gaubert, Stéphane Balazs, Ervin Tepfer, Mark Viral interference with gene silencing in non-transgenic or transgenic plants |
description |
Various agronomically interesting traits have been obtained by inducing post-transcriptional gene silencing (PTGS) in transgenic cultivated crops. Some plant cultivars that were created by interspecific crosses also display PTGS-induced phenotypes. The finding that many plant viruses have the ability to counteract PTGS raises the possibility that viral infection may cause the loss of PTGS-induced traits in transgenic as well as non-transgenic plants. We addressed this question by studying virus-specific differences in the interference with silencing of the chs-A gene in non-transgenic RedStar-type petunia whose flowers display variegated flowers resulting from natural PTGS of the chs-A gene. Our results show that potato potyvirus Y (PVY), tobacco etch potyvirus (TEV) and cucumber mosaic cucumovirus (CMV), which are known to inhibit PTGS, induce specific phenotypic changes resulting from spatially-regulated interference between viral infection and PTGS of the chs-A gene. However, PTGS breakdown was not total, and the pattern observed depended on which virus was involved. This suggests that the degree of interference with PTGS will depend on the pattern of expression of the silenced gene and on that of the accumulation of the potentially interfering virus. We are currently studying the effects of viral infection by TEV or CMV on PTGS-induced resistance to PVY in transgenic tobacco lines expressing a PVY coat protein gene. Preliminary results suggest that the two viruses have different effects. These results show that viral infections could lead to the loss of agronomically interesting traits obtained by PTGS in either transgenic or non-transgenic plants, such as flower color, virus-resistance, or modified seed oil composition. |
format |
conference_item |
topic_facet |
H20 - Maladies des plantes |
author |
Teycheney, Pierre-Yves Gaubert, Stéphane Balazs, Ervin Tepfer, Mark |
author_facet |
Teycheney, Pierre-Yves Gaubert, Stéphane Balazs, Ervin Tepfer, Mark |
author_sort |
Teycheney, Pierre-Yves |
title |
Viral interference with gene silencing in non-transgenic or transgenic plants |
title_short |
Viral interference with gene silencing in non-transgenic or transgenic plants |
title_full |
Viral interference with gene silencing in non-transgenic or transgenic plants |
title_fullStr |
Viral interference with gene silencing in non-transgenic or transgenic plants |
title_full_unstemmed |
Viral interference with gene silencing in non-transgenic or transgenic plants |
title_sort |
viral interference with gene silencing in non-transgenic or transgenic plants |
publisher |
s.n. |
url |
http://agritrop.cirad.fr/510776/ |
work_keys_str_mv |
AT teycheneypierreyves viralinterferencewithgenesilencinginnontransgenicortransgenicplants AT gaubertstephane viralinterferencewithgenesilencinginnontransgenicortransgenicplants AT balazservin viralinterferencewithgenesilencinginnontransgenicortransgenicplants AT tepfermark viralinterferencewithgenesilencinginnontransgenicortransgenicplants |
_version_ |
1758019615496077312 |