Developmental changes in carboxylase activities in vitro cultured coconut zygotic embryos: comparison with corresponding activities in seedlings
Phosphoenolpyruvate Carboxylase (PEPC; EC: 4.1.1.31) and Ribulose 1.5-bisphosphate Carboxylase/Oxygenase (RubisCO; EC: 4.1.1.39) enzyme specific activities were measured during the #in vitro# development of coconut (#Cocos nucifera# L.) zygotic mature embryos into plantlets and compared with those of palms produced by conventional seed germination. At the time of initiation of germination, high PEPC and low RubisCO activities were measured in both cultured and conventionally germinated embryos, thus indicating an anaplerotic C02 fixation. During both #in vitro# and #in planta# development, RubisCO progressively took over and became the main route for inorganic carbon fixation. The #in vitro#-grown coconut plantlets showed a faster decrease in their PEPC/RubisCO ratio than the seedlings, suggesting that an earlier transition from a heterotrophic to an autotrophic mode on carbon fixation takes place in the #in vitro#-denved material. Just before acclimatization, the RubisCO activity in #in vitro#-derived plantlets (2.83 pmol C02h-1mg-1 TSP) was lower than that in seedlings (6.98 pmol C02h-1 mg-1 TSP ) for the same age. Nevertheless, after acclimatization, RubisCO activities were comparable in both #in vitro# and #in planta# germinated materiel.
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Subjects: | F60 - Physiologie et biochimie végétale, Cocos nucifera, culture d'embryon, activité enzymatique, phosphoenolpyruvate carboxylase, rubisco, nutrition des plantes, photosynthèse, http://aims.fao.org/aos/agrovoc/c_1716, http://aims.fao.org/aos/agrovoc/c_2544, http://aims.fao.org/aos/agrovoc/c_2604, http://aims.fao.org/aos/agrovoc/c_29524, http://aims.fao.org/aos/agrovoc/c_33542, http://aims.fao.org/aos/agrovoc/c_16379, http://aims.fao.org/aos/agrovoc/c_5812, |
Online Access: | http://agritrop.cirad.fr/389619/ http://agritrop.cirad.fr/389619/1/389619.pdf |
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dig-cirad-fr-3896192024-01-27T22:59:02Z http://agritrop.cirad.fr/389619/ http://agritrop.cirad.fr/389619/ Developmental changes in carboxylase activities in vitro cultured coconut zygotic embryos: comparison with corresponding activities in seedlings. Triques K., Rival Alain, Beulé Thierry, Dussert Stéphane, Hocher Valérie, Verdeil Jean-Luc, Hamon Serge. 1997. Plant Cell, Tissue and Organ Culture, 49 : 227-231.https://doi.org/10.1023/A:1005856212846 <https://doi.org/10.1023/A:1005856212846> Developmental changes in carboxylase activities in vitro cultured coconut zygotic embryos: comparison with corresponding activities in seedlings Triques, K. Rival, Alain Beulé, Thierry Dussert, Stéphane Hocher, Valérie Verdeil, Jean-Luc Hamon, Serge eng 1997 Plant Cell, Tissue and Organ Culture F60 - Physiologie et biochimie végétale Cocos nucifera culture d'embryon activité enzymatique phosphoenolpyruvate carboxylase rubisco nutrition des plantes photosynthèse http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_2544 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_29524 http://aims.fao.org/aos/agrovoc/c_33542 http://aims.fao.org/aos/agrovoc/c_16379 http://aims.fao.org/aos/agrovoc/c_5812 Phosphoenolpyruvate Carboxylase (PEPC; EC: 4.1.1.31) and Ribulose 1.5-bisphosphate Carboxylase/Oxygenase (RubisCO; EC: 4.1.1.39) enzyme specific activities were measured during the #in vitro# development of coconut (#Cocos nucifera# L.) zygotic mature embryos into plantlets and compared with those of palms produced by conventional seed germination. At the time of initiation of germination, high PEPC and low RubisCO activities were measured in both cultured and conventionally germinated embryos, thus indicating an anaplerotic C02 fixation. During both #in vitro# and #in planta# development, RubisCO progressively took over and became the main route for inorganic carbon fixation. The #in vitro#-grown coconut plantlets showed a faster decrease in their PEPC/RubisCO ratio than the seedlings, suggesting that an earlier transition from a heterotrophic to an autotrophic mode on carbon fixation takes place in the #in vitro#-denved material. Just before acclimatization, the RubisCO activity in #in vitro#-derived plantlets (2.83 pmol C02h-1mg-1 TSP) was lower than that in seedlings (6.98 pmol C02h-1 mg-1 TSP ) for the same age. Nevertheless, after acclimatization, RubisCO activities were comparable in both #in vitro# and #in planta# germinated materiel. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/389619/1/389619.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1023/A:1005856212846 10.1023/A:1005856212846 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=89780 info:eu-repo/semantics/altIdentifier/doi/10.1023/A:1005856212846 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1023/A:1005856212846 |
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F60 - Physiologie et biochimie végétale Cocos nucifera culture d'embryon activité enzymatique phosphoenolpyruvate carboxylase rubisco nutrition des plantes photosynthèse http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_2544 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_29524 http://aims.fao.org/aos/agrovoc/c_33542 http://aims.fao.org/aos/agrovoc/c_16379 http://aims.fao.org/aos/agrovoc/c_5812 F60 - Physiologie et biochimie végétale Cocos nucifera culture d'embryon activité enzymatique phosphoenolpyruvate carboxylase rubisco nutrition des plantes photosynthèse http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_2544 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_29524 http://aims.fao.org/aos/agrovoc/c_33542 http://aims.fao.org/aos/agrovoc/c_16379 http://aims.fao.org/aos/agrovoc/c_5812 |
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F60 - Physiologie et biochimie végétale Cocos nucifera culture d'embryon activité enzymatique phosphoenolpyruvate carboxylase rubisco nutrition des plantes photosynthèse http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_2544 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_29524 http://aims.fao.org/aos/agrovoc/c_33542 http://aims.fao.org/aos/agrovoc/c_16379 http://aims.fao.org/aos/agrovoc/c_5812 F60 - Physiologie et biochimie végétale Cocos nucifera culture d'embryon activité enzymatique phosphoenolpyruvate carboxylase rubisco nutrition des plantes photosynthèse http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_2544 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_29524 http://aims.fao.org/aos/agrovoc/c_33542 http://aims.fao.org/aos/agrovoc/c_16379 http://aims.fao.org/aos/agrovoc/c_5812 Triques, K. Rival, Alain Beulé, Thierry Dussert, Stéphane Hocher, Valérie Verdeil, Jean-Luc Hamon, Serge Developmental changes in carboxylase activities in vitro cultured coconut zygotic embryos: comparison with corresponding activities in seedlings |
description |
Phosphoenolpyruvate Carboxylase (PEPC; EC: 4.1.1.31) and Ribulose 1.5-bisphosphate Carboxylase/Oxygenase (RubisCO; EC: 4.1.1.39) enzyme specific activities were measured during the #in vitro# development of coconut (#Cocos nucifera# L.) zygotic mature embryos into plantlets and compared with those of palms produced by conventional seed germination. At the time of initiation of germination, high PEPC and low RubisCO activities were measured in both cultured and conventionally germinated embryos, thus indicating an anaplerotic C02 fixation. During both #in vitro# and #in planta# development, RubisCO progressively took over and became the main route for inorganic carbon fixation. The #in vitro#-grown coconut plantlets showed a faster decrease in their PEPC/RubisCO ratio than the seedlings, suggesting that an earlier transition from a heterotrophic to an autotrophic mode on carbon fixation takes place in the #in vitro#-denved material. Just before acclimatization, the RubisCO activity in #in vitro#-derived plantlets (2.83 pmol C02h-1mg-1 TSP) was lower than that in seedlings (6.98 pmol C02h-1 mg-1 TSP ) for the same age. Nevertheless, after acclimatization, RubisCO activities were comparable in both #in vitro# and #in planta# germinated materiel. |
format |
article |
topic_facet |
F60 - Physiologie et biochimie végétale Cocos nucifera culture d'embryon activité enzymatique phosphoenolpyruvate carboxylase rubisco nutrition des plantes photosynthèse http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_2544 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_29524 http://aims.fao.org/aos/agrovoc/c_33542 http://aims.fao.org/aos/agrovoc/c_16379 http://aims.fao.org/aos/agrovoc/c_5812 |
author |
Triques, K. Rival, Alain Beulé, Thierry Dussert, Stéphane Hocher, Valérie Verdeil, Jean-Luc Hamon, Serge |
author_facet |
Triques, K. Rival, Alain Beulé, Thierry Dussert, Stéphane Hocher, Valérie Verdeil, Jean-Luc Hamon, Serge |
author_sort |
Triques, K. |
title |
Developmental changes in carboxylase activities in vitro cultured coconut zygotic embryos: comparison with corresponding activities in seedlings |
title_short |
Developmental changes in carboxylase activities in vitro cultured coconut zygotic embryos: comparison with corresponding activities in seedlings |
title_full |
Developmental changes in carboxylase activities in vitro cultured coconut zygotic embryos: comparison with corresponding activities in seedlings |
title_fullStr |
Developmental changes in carboxylase activities in vitro cultured coconut zygotic embryos: comparison with corresponding activities in seedlings |
title_full_unstemmed |
Developmental changes in carboxylase activities in vitro cultured coconut zygotic embryos: comparison with corresponding activities in seedlings |
title_sort |
developmental changes in carboxylase activities in vitro cultured coconut zygotic embryos: comparison with corresponding activities in seedlings |
url |
http://agritrop.cirad.fr/389619/ http://agritrop.cirad.fr/389619/1/389619.pdf |
work_keys_str_mv |
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_version_ |
1792491197847044096 |