Classification of plant trypanosomatids (Phytomonas spp.) : parity between random-primer DNA typing and multilocus enzyme electrophoresis
The genetic polymorphism of 30 isolates of plant trypanosomatids colloquially referred to as plant trypanosomes was assayed by means of RAPD. The principle objectives of this study were to assess the discriminative power of RAPD analysis for studying plant trypanosomes and to determine whether the results obtained were comparable with those from a previous isoenzyme (MLEE) study. The principle groups of plant trypanosomes identified previously by isoenzyme analysis - intraphloemic trypanosomes, intralaticiferous trypanosomes and trypanosomes isolated from fruits - were also clearly separated by the RAPD technique. Moreover, the results showed a fair parity between MLEE and RAPD data (coefficient of correlation = 0.84) and the two techniques have comparable discriminative ability. Most of the separation revealed by the two techniques between the clusters was associated with major biological properties. However, the RAPD technique gave a more coherent separation than MLEE because the intraphloemic isolates, which were biologically similar in terms of their specific localization in the sieve tubes of the plant, were found to be in closer groups by the RAPD. For both techniques, the existence of the main clusters was correlated with the existence of synapomorphic characters, which could be used as powerful tools in taxonomy and epidemiology.
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Subjects: | H10 - Ravageurs des plantes, F30 - Génétique et amélioration des plantes, Phytomonas, PCR, adn, isoenzyme, technique analytique, taxonomie, http://aims.fao.org/aos/agrovoc/c_26618, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_2347, http://aims.fao.org/aos/agrovoc/c_15987, http://aims.fao.org/aos/agrovoc/c_1513, http://aims.fao.org/aos/agrovoc/c_7631, |
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dig-cirad-fr-3895742024-01-27T22:57:41Z http://agritrop.cirad.fr/389574/ http://agritrop.cirad.fr/389574/ Classification of plant trypanosomatids (Phytomonas spp.) : parity between random-primer DNA typing and multilocus enzyme electrophoresis. Muller Emmanuelle, Gargani Daniel, Banuls A.L., Tibayrenc Michel, Dollet Michel. 1997. Parasitology, 115 : 403-409.https://doi.org/10.1017/S0031182097001522 <https://doi.org/10.1017/S0031182097001522> Classification of plant trypanosomatids (Phytomonas spp.) : parity between random-primer DNA typing and multilocus enzyme electrophoresis Muller, Emmanuelle Gargani, Daniel Banuls, A.L. Tibayrenc, Michel Dollet, Michel eng 1997 Parasitology H10 - Ravageurs des plantes F30 - Génétique et amélioration des plantes Phytomonas PCR adn isoenzyme technique analytique taxonomie http://aims.fao.org/aos/agrovoc/c_26618 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_15987 http://aims.fao.org/aos/agrovoc/c_1513 http://aims.fao.org/aos/agrovoc/c_7631 The genetic polymorphism of 30 isolates of plant trypanosomatids colloquially referred to as plant trypanosomes was assayed by means of RAPD. The principle objectives of this study were to assess the discriminative power of RAPD analysis for studying plant trypanosomes and to determine whether the results obtained were comparable with those from a previous isoenzyme (MLEE) study. The principle groups of plant trypanosomes identified previously by isoenzyme analysis - intraphloemic trypanosomes, intralaticiferous trypanosomes and trypanosomes isolated from fruits - were also clearly separated by the RAPD technique. Moreover, the results showed a fair parity between MLEE and RAPD data (coefficient of correlation = 0.84) and the two techniques have comparable discriminative ability. Most of the separation revealed by the two techniques between the clusters was associated with major biological properties. However, the RAPD technique gave a more coherent separation than MLEE because the intraphloemic isolates, which were biologically similar in terms of their specific localization in the sieve tubes of the plant, were found to be in closer groups by the RAPD. For both techniques, the existence of the main clusters was correlated with the existence of synapomorphic characters, which could be used as powerful tools in taxonomy and epidemiology. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/389574/1/389574.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1017/S0031182097001522 10.1017/S0031182097001522 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=89177 info:eu-repo/semantics/altIdentifier/doi/10.1017/S0031182097001522 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1017/S0031182097001522 |
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H10 - Ravageurs des plantes F30 - Génétique et amélioration des plantes Phytomonas PCR adn isoenzyme technique analytique taxonomie http://aims.fao.org/aos/agrovoc/c_26618 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_15987 http://aims.fao.org/aos/agrovoc/c_1513 http://aims.fao.org/aos/agrovoc/c_7631 H10 - Ravageurs des plantes F30 - Génétique et amélioration des plantes Phytomonas PCR adn isoenzyme technique analytique taxonomie http://aims.fao.org/aos/agrovoc/c_26618 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_15987 http://aims.fao.org/aos/agrovoc/c_1513 http://aims.fao.org/aos/agrovoc/c_7631 |
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H10 - Ravageurs des plantes F30 - Génétique et amélioration des plantes Phytomonas PCR adn isoenzyme technique analytique taxonomie http://aims.fao.org/aos/agrovoc/c_26618 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_15987 http://aims.fao.org/aos/agrovoc/c_1513 http://aims.fao.org/aos/agrovoc/c_7631 H10 - Ravageurs des plantes F30 - Génétique et amélioration des plantes Phytomonas PCR adn isoenzyme technique analytique taxonomie http://aims.fao.org/aos/agrovoc/c_26618 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_15987 http://aims.fao.org/aos/agrovoc/c_1513 http://aims.fao.org/aos/agrovoc/c_7631 Muller, Emmanuelle Gargani, Daniel Banuls, A.L. Tibayrenc, Michel Dollet, Michel Classification of plant trypanosomatids (Phytomonas spp.) : parity between random-primer DNA typing and multilocus enzyme electrophoresis |
description |
The genetic polymorphism of 30 isolates of plant trypanosomatids colloquially referred to as plant trypanosomes was assayed by means of RAPD. The principle objectives of this study were to assess the discriminative power of RAPD analysis for studying plant trypanosomes and to determine whether the results obtained were comparable with those from a previous isoenzyme (MLEE) study. The principle groups of plant trypanosomes identified previously by isoenzyme analysis - intraphloemic trypanosomes, intralaticiferous trypanosomes and trypanosomes isolated from fruits - were also clearly separated by the RAPD technique. Moreover, the results showed a fair parity between MLEE and RAPD data (coefficient of correlation = 0.84) and the two techniques have comparable discriminative ability. Most of the separation revealed by the two techniques between the clusters was associated with major biological properties. However, the RAPD technique gave a more coherent separation than MLEE because the intraphloemic isolates, which were biologically similar in terms of their specific localization in the sieve tubes of the plant, were found to be in closer groups by the RAPD. For both techniques, the existence of the main clusters was correlated with the existence of synapomorphic characters, which could be used as powerful tools in taxonomy and epidemiology. |
format |
article |
topic_facet |
H10 - Ravageurs des plantes F30 - Génétique et amélioration des plantes Phytomonas PCR adn isoenzyme technique analytique taxonomie http://aims.fao.org/aos/agrovoc/c_26618 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_15987 http://aims.fao.org/aos/agrovoc/c_1513 http://aims.fao.org/aos/agrovoc/c_7631 |
author |
Muller, Emmanuelle Gargani, Daniel Banuls, A.L. Tibayrenc, Michel Dollet, Michel |
author_facet |
Muller, Emmanuelle Gargani, Daniel Banuls, A.L. Tibayrenc, Michel Dollet, Michel |
author_sort |
Muller, Emmanuelle |
title |
Classification of plant trypanosomatids (Phytomonas spp.) : parity between random-primer DNA typing and multilocus enzyme electrophoresis |
title_short |
Classification of plant trypanosomatids (Phytomonas spp.) : parity between random-primer DNA typing and multilocus enzyme electrophoresis |
title_full |
Classification of plant trypanosomatids (Phytomonas spp.) : parity between random-primer DNA typing and multilocus enzyme electrophoresis |
title_fullStr |
Classification of plant trypanosomatids (Phytomonas spp.) : parity between random-primer DNA typing and multilocus enzyme electrophoresis |
title_full_unstemmed |
Classification of plant trypanosomatids (Phytomonas spp.) : parity between random-primer DNA typing and multilocus enzyme electrophoresis |
title_sort |
classification of plant trypanosomatids (phytomonas spp.) : parity between random-primer dna typing and multilocus enzyme electrophoresis |
url |
http://agritrop.cirad.fr/389574/ http://agritrop.cirad.fr/389574/1/389574.pdf |
work_keys_str_mv |
AT mulleremmanuelle classificationofplanttrypanosomatidsphytomonassppparitybetweenrandomprimerdnatypingandmultilocusenzymeelectrophoresis AT garganidaniel classificationofplanttrypanosomatidsphytomonassppparitybetweenrandomprimerdnatypingandmultilocusenzymeelectrophoresis AT banulsal classificationofplanttrypanosomatidsphytomonassppparitybetweenrandomprimerdnatypingandmultilocusenzymeelectrophoresis AT tibayrencmichel classificationofplanttrypanosomatidsphytomonassppparitybetweenrandomprimerdnatypingandmultilocusenzymeelectrophoresis AT dolletmichel classificationofplanttrypanosomatidsphytomonassppparitybetweenrandomprimerdnatypingandmultilocusenzymeelectrophoresis |
_version_ |
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